LOCUS       sequence-488093-        2685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Arabinose-Inducible promoter, including ribosome 
            binding site. Lacks activator protein, AraC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_FULLPRM_Para
FEATURES             Location/Qualifiers
     source          1..2685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        38..322
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     245..264
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     promoter        complement(390..408)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(391..408)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(424..440)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(424..440)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(555..1143)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(635..654)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1258..1360
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1361..2020
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1427..1446)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2133..2588
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2220..2239)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2430..2451
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2607..2624
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2608..2624
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2631..2650
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2631..2649
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcggga gacttttcat actcccacca ttcagagaag aaaccaattg tccatattgc
       61 atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccca accggtaacc
      121 ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac
      181 aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt
      241 tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg
      301 caactctcta ctgtttctcc atacccgttt ttttggatgg agtgaaacga atgcgagacc
      361 actcatcgcc caatcactag tgaattcgtt ctatagtgtc acctaaatac catagagccg
      421 ttggtcatag ctgtttcctg tccaggcatc atgacacggt tatccacaga atcaggggat
      481 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      541 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      601 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      661 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      721 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      781 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      841 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      901 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      961 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1021 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1081 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1141 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1201 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttgtgatga
     1261 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
     1321 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     1381 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     1441 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     1501 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     1561 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     1621 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     1681 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     1741 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtgtcagc
     1801 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     1861 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     1921 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     1981 attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg
     2041 gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga tgaatggcag
     2101 aaattcgatg ataagctgtc aaacatgatg ttacgcgccc tgtagcggcg cattaagcgc
     2161 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     2221 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     2281 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     2341 acttgattag ggtgatggtt cacgtagtgg gccagcgccc tgatagacgg tttttcgccc
     2401 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     2461 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     2521 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     2581 tacaatttta acattgtaga aacgcatgta aaacgacggc cagtgaattg taatacgact
     2641 cactataggg tgaattccgc ttaggacctt agatattgat ctcaa
//
