LOCUS       sequence-488097-        2448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Temperature-Inducible promoter, including ribosome 
            binding site. Lacks repressor, cI857..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_FULLPRM_PR
FEATURES             Location/Qualifiers
     source          1..2448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             15..23
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(153..171)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(154..171)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(187..203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(187..203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(318..906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(398..417)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1021..1123
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1124..1783
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1190..1209)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1896..2351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1983..2002)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2193..2214
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2370..2387
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2371..2387
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2394..2413
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2394..2412
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcgcat tacaacctcc ttagtacatg caaccattat caccgccaga ggtaaaatag
       61 tcaacacgca cggtgttaga tatttatccc ttgcggtgat agatttaacg tactcccgag
      121 accactcatc gcccaatcac tagtgaattc gttctatagt gtcacctaaa taccatagag
      181 ccgttggtca tagctgtttc ctgtccaggc atcatgacac ggttatccac agaatcaggg
      241 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      301 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      361 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      421 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      481 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      541 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      601 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      661 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      721 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
      781 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      841 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      901 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
      961 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttgtga
     1021 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
     1081 attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1141 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1201 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1261 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1321 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1381 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1441 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1501 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtgtc
     1561 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1621 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1681 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     1741 tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta
     1801 ttggtgccct taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg
     1861 cagaaattcg atgataagct gtcaaacatg atgttacgcg ccctgtagcg gcgcattaag
     1921 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     1981 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     2041 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     2101 aaaacttgat tagggtgatg gttcacgtag tgggccagcg ccctgataga cggtttttcg
     2161 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     2221 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     2281 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     2341 gcttacaatt ttaacattgt agaaacgcat gtaaaacgac ggccagtgaa ttgtaatacg
     2401 actcactata gggtgaattc cgcttaggac cttagatatt gatctcaa
//
