LOCUS       sequence-488098-        2404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 T7 RNA polymerase-dependent promoter, including 
            ribosome binding site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_FULLPRM_PT7
FEATURES             Location/Qualifiers
     source          1..2404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(109..127)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(110..127)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(143..159)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(143..159)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(274..862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(354..373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        977..1079
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1080..1739
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1146..1165)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1852..2307
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1939..1958)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2149..2170
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2326..2343
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2327..2343
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2350..2368
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcggga gtaatacgac tcactatagt ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataaa tgcgagacca ctcatcgccc aatcactagt gaattcgttc tatagtgtca
      121 cctaaatacc atagagccgt tggtcatagc tgtttcctgt ccaggcatca tgacacggtt
      181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      241 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      961 ctagatcctt ttgtgatgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa
     1021 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
     1081 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     1141 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     1201 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     1261 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     1321 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     1381 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     1441 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     1501 atatgttttt cgtgtcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     1561 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     1621 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     1681 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
     1741 ttttttaagg cagttattgg tgcccttaaa cgcctggttg ctacgcctga ataagtgata
     1801 ataagcggat gaatggcaga aattcgatga taagctgtca aacatgatgt tacgcgccct
     1861 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     1921 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     1981 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     2041 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccagcgccct
     2101 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     2161 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     2221 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     2281 ttaacaaaat attaacgctt acaattttaa cattgtagaa acgcatgtaa aacgacggcc
     2341 agtgaattgt aatacgactc actatagggt gaattccgct taggacctta gatattgatc
     2401 tcaa
//
