LOCUS       sequence-48810-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Glover CP, Bienemann AS, Heywood DJ, Cosgrave AS, Uney JB
  TITLE     Adenoviral-mediated, high-level, cell-specific transgene expression:
            a SYN1-WPRE cassette mediates increased transgene expression with no
            loss of neuron specificity.
  JOURNAL   Mol Ther. 2002 May;5(5 Pt 1):509-16.
  PUBMED    11991741
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther.";
            date: "2002-05"; volume: "5(5 Pt 1)"; pages: "509-16"
COMMENT     Sequence Label: pXCX.Syn.EGFP.WPRE
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             101..112
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     510..530
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(667..688)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aggcggccca aagggagatc cgactcgtct gagggcgaag gcgaagacgc ggaagaggcc
       61 gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg attgagggcc gaagggacgt
      121 agcagaagga cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc catggaaagg
      181 acgtcagctt ccccgacaac accacggaat tgtcagtgcc caacagccga gcccctgtcc
      241 agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc aatagggagg gggaaagcga
      301 aagtcccgga aaggagctga caggtggtgg caatgcccca accagtgggg gttgcgtcag
      361 caaacacagt gcacaccacg ccacgttgcc tgacaacggg ccacaactcc tcataaagag
      421 acagcaacca ggatttatac aaggaggaga aaatgaaagc catacgggaa gcaatagcat
      481 gatacaaagg cattaaagca gcgtatccac atagcgtaaa aggagcaaca tagttaagaa
      541 taccagtcaa tctttcacaa attttgtaat ccagaggttg attgtcgacg gatcctctag
      601 atgcatgctc gagcggccgc tttacttgta cagctcgtcc atgccgagag tgatcccggc
      661 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
      721 ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
      781 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
      841 cttgaagttc accttgatgc cgttcttctg cttgt
//
