LOCUS       sequence-488100-        2395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Strong, constitutive promoter, not including ribosome
            binding site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_PARTPRM_Pem7
FEATURES             Location/Qualifiers
     source          1..2395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..59
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(100..118)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(101..118)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(134..150)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(134..150)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(265..853)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(345..364)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        968..1070
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1071..1730
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1137..1156)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1843..2298
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1930..1949)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2140..2161
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2317..2334
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2318..2334
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2341..2360
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2341..2359
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcggga ggttgacaat taatcatcgg catagtatat cggcatagta taatacgacc
       61 aaacgagacc actcatcgcc caatcactag tgaattcgtt ctatagtgtc acctaaatac
      121 catagagccg ttggtcatag ctgtttcctg tccaggcatc atgacacggt tatccacaga
      181 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      241 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      301 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      361 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      421 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      481 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      541 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      601 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      661 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
      721 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
      781 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
      841 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
      901 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
      961 tttgtgatga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac
     1021 cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa
     1081 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
     1141 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
     1201 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
     1261 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga
     1321 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
     1381 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
     1441 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
     1501 tcgtgtcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
     1561 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
     1621 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
     1681 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag
     1741 gcagttattg gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga
     1801 tgaatggcag aaattcgatg ataagctgtc aaacatgatg ttacgcgccc tgtagcggcg
     1861 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     1921 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     1981 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     2041 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccagcgccc tgatagacgg
     2101 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     2161 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     2221 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     2281 tattaacgct tacaatttta acattgtaga aacgcatgta aaacgacggc cagtgaattg
     2341 taatacgact cactataggg tgaattccgc ttaggacctt agatattgat ctcaa
//
