LOCUS       sequence-488102-        2461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 IPTG/Lactose-Inducible promoter, not including 
            ribosome binding site. Lacks repressor, LacI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_PARTPRM_Plac
FEATURES             Location/Qualifiers
     source          1..2461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    32..53
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(68..73,74..91,92..98)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    106..122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(166..184)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(167..184)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(200..216)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(200..216)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(331..919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(411..430)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1034..1136
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1137..1796
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1203..1222)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1909..2364
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1996..2015)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2206..2227
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2383..2400
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2384..2400
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2407..2426
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2407..2425
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcggga gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
       61 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
      121 aatttcaaac gagaccactc atcgcccaat cactagtgaa ttcgttctat agtgtcacct
      181 aaataccata gagccgttgg tcatagctgt ttcctgtcca ggcatcatga cacggttatc
      241 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      301 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      361 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      421 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      481 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
      541 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
      601 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
      661 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      721 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
      781 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
      841 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
      901 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
      961 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1021 gatccttttg tgatgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat
     1081 cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg
     1141 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     1201 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     1261 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     1321 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
     1381 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     1441 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     1501 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     1561 tgtttttcgt gtcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     1621 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     1681 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     1741 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt
     1801 tttaaggcag ttattggtgc ccttaaacgc ctggttgcta cgcctgaata agtgataata
     1861 agcggatgaa tggcagaaat tcgatgataa gctgtcaaac atgatgttac gcgccctgta
     1921 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     1981 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     2041 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     2101 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca gcgccctgat
     2161 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     2221 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     2281 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     2341 acaaaatatt aacgcttaca attttaacat tgtagaaacg catgtaaaac gacggccagt
     2401 gaattgtaat acgactcact atagggtgaa ttccgcttag gaccttagat attgatctca
     2461 a
//
