LOCUS       sequence-488103-        2659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Arabinose-Inducible promoter, not including ribosome 
            binding site. Lacks activator protein, AraC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_PARTPRM_Para
FEATURES             Location/Qualifiers
     source          1..2659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        38..322
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     245..264
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     promoter        complement(364..382)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(365..382)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(398..414)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(398..414)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(529..1117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(609..628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1232..1334
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1335..1994
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1401..1420)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2107..2562
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2194..2213)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2404..2425
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2581..2598
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2582..2598
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2605..2624
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2605..2623
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcggga gacttttcat actcccacca ttcagagaag aaaccaattg tccatattgc
       61 atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccca accggtaacc
      121 ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac
      181 aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt
      241 tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg
      301 caactctcta ctgtttctcc atacaaacga gaccactcat cgcccaatca ctagtgaatt
      361 cgttctatag tgtcacctaa ataccataga gccgttggtc atagctgttt cctgtccagg
      421 catcatgaca cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      481 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      541 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      601 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      661 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      721 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      781 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      841 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      901 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      961 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1021 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1081 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1141 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1201 aaaaaggatc ttcacctaga tccttttgtg atgatcggca cgtaagaggt tccaactttc
     1261 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
     1321 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
     1381 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
     1441 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     1501 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg
     1561 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     1621 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     1681 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     1741 aagggtttat tgagaatatg tttttcgtgt cagccaatcc ctgggtgagt ttcaccagtt
     1801 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     1861 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     1921 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     1981 agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggttgctacg
     2041 cctgaataag tgataataag cggatgaatg gcagaaattc gatgataagc tgtcaaacat
     2101 gatgttacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     2161 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     2221 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     2281 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     2341 gtgggccagc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     2401 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     2461 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     2521 aatttaacgc gaattttaac aaaatattaa cgcttacaat tttaacattg tagaaacgca
     2581 tgtaaaacga cggccagtga attgtaatac gactcactat agggtgaatt ccgcttagga
     2641 ccttagatat tgatctcaa
//
