LOCUS       sequence-488113-        9989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mScarlet-i and SBP tagged human SPARC for protein 
            visualisation and use in the RUSH trafficking system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Thompson G, Hoyle A, Lewis PA, Prada-Sanchez ME, Swift J, Heesom K, 
            Lowe M, Stephens D, Stevenson NL
  TITLE     Multiple golgins are required to support extracellular matrix 
            secretion, modification, and assembly.
  JOURNAL   J Cell Biol. 2025 Oct 6;224(10):e202411167. doi: 
            10.1083/jcb.202411167. Epub 2025 Aug 18.
  PUBMED    40824304
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2025-10-6"; volume: "224(10):e202411167. doi"; pages:
            " 10.1083/jcb.202411167. Epub 2025 Aug 18"
COMMENT     Sequence Label: pLVXNeo_SPARC-mSc-SBP
FEATURES             Location/Qualifiers
     source          1..9989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3681..3794
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     misc_feature    join(4563..5137,5138..5140,5141..5149)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4730..4747)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4957..4976
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5150..5944
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5204..5223)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5814..5833
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    5958..6546
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6011..6031)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6429..6440)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6753..7386
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7514..7530)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7514..7530)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7527..7549)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7538..7554
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7562..7568,7569..7586,7587..7592))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7607..7628
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7745..7762)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7916..8504)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7996..8015)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8675..9466,9467..9535))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9298..9317
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9536..9640)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9688..9822
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9725..9744)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9779..9798
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatca gcaccatgag ggcctggata ttctttctcc
     2761 tttgcctggc cgggagggcg ttggcagccc ctcagcaaga agcactacct gatgagacag
     2821 aggttgttga agaaactgtc gcagaggtga ctgaggtatc tgtcggagct aatcctgtcc
     2881 aggttgaagt aggagaattt gatgatggtg cagaggaaac ggaagaagag gtggtggcgg
     2941 aaaatccctg ccaaaatcat cactgcaaac acggaaaagt gtgtgagtta gatgagaaca
     3001 atactcccat gtgcgtgtgt caggacccaa ctagctgccc agcccctatt ggcgagtttg
     3061 agaaagtctg tagcaatgac aacaagacct tcgattctag ttgtcatttt tttgccacaa
     3121 agtgcacttt ggagggcacg aagaagggtc acaagctcca tctggactac atcgggcctt
     3181 gtaaatacat tcccccttgt ctggattctg agctgaccga attccccctg cgcatgcggg
     3241 attggctcaa gaacgtcttg gtcactctgt atgagaggga tgaagacaac aaccttctga
     3301 ctgagaagca gaagttacgg gttaagaaga tccatgagaa tgagaaacgc ctggaggcag
     3361 gagaccaccc agtggagcta ttagcccggg actttgagaa aaattataac atgtatattt
     3421 ttcctgtaca ctggcagttc gggcagcttg accagcatcc aattgacggg tacctctcgc
     3481 acaccgagct ggctccatta cgtgctccgc tcatccccat ggaacattgc acaactagat
     3541 tttttgaaac gtgtgacctg gacaatgaca agtacatcgc tctcgatgag tgggctggct
     3601 gctttggaat caaacaaaag gatatcgaca aggatcttgt cataggaagt gctggctccg
     3661 ctgctggttc tggcgaattc atggatgaaa agaccaccgg atggagaggt gggcatgttg
     3721 ttgaaggtct tgctggcgaa ttggagcagc tgcgagccag actcgagcat cacccacaag
     3781 ggcaaagaga gccggaagga aaatcaagtg ggtctgggtc agagagcaaa agcaccatgg
     3841 tgagcaaagg agaagcagtg attaaagagt ttatgcggtt caaagtgcac atggagggct
     3901 caatgaacgg acacgaattc gaaattgagg gagagggaga aggccgaccc tacgagggca
     3961 cccagacagc caaacttaag gtgacgaagg gtgggccact gcccttctca tgggacatcc
     4021 tatcccctca gttcatgtac ggctccaggg ccttcataaa gcaccccgcc gacatcccgg
     4081 attattataa gcagagcttc ccagagggct tcaagtggga aagggtgatg aacttcgaag
     4141 acggcggggc agtgacagtg acacaggaca catccctgga ggatggcacc ctgatctaca
     4201 aggtgaaact ccgcgggaca aacttccctc ctgatggtcc cgtaatgcag aaaaaaacaa
     4261 tggggtggga agcgtccacc gagcggttgt atccggagga cggggtgctg aagggcgata
     4321 ttaagatggc cctgcgtctg aaagatggag gccgctacct ggcggacttt aagactacct
     4381 ataaagccaa gaaacccgtc cagatgccag gcgcctacaa tgtcgaccgc aagttggaca
     4441 taacctccca caacgaggat tataccgtgg tggaacagta cgaaagaagc gagggccgac
     4501 acagtacagg tggcatggac gagctctaca agtgaatcca cctagagcgg ccgcggatcc
     4561 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     4621 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     4681 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     4741 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     4801 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     4861 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     4921 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     4981 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     5041 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     5101 ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaacca tggctgaaca
     5161 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     5221 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     5281 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     5341 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     5401 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5461 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5521 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     5581 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5641 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     5701 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5761 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5821 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5881 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5941 ctgaacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     6001 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6061 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6121 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6181 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6241 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6301 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     6361 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     6421 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6481 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6541 ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag
     6601 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     6661 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     6721 tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag
     6781 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
     6841 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     6901 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     6961 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
     7021 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
     7081 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
     7141 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     7201 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7261 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7321 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7381 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7441 tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct agctagagct
     7501 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7561 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7621 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7681 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7741 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7801 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7861 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7921 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7981 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8041 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8101 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8161 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8221 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8281 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8341 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8401 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8461 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8521 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8581 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8641 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8701 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8761 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8821 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8881 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8941 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9001 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9061 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9121 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9181 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9241 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9301 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9361 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9421 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9481 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9541 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9601 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9661 cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa
     9721 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9781 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
     9841 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
     9901 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
     9961 tgttaaatat gtactacaaa cttagtagt
//
