LOCUS       sequence-488119-       14394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary Plasmid for plant transformation expressing RUBY from the 35S
            promoter and the terminator from the Arabidopsis thaliana HSP gene. 
            Kanamycin plant selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14394)
  TITLE     Monitoring the stability of transgene expression in lettuce using 
            the RUBY reporter.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJD849
FEATURES             Location/Qualifiers
     source          1..14394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..711
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     648..668
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             720..4670
                     /label=RUBY
     CDS             2220..2276
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3111..3167
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     promoter        5373..5552
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        5405..5588
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5609..6403
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5663..5682)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6273..6292
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6793..7045
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7590..7607)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7590..7606)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7599..7621)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7828..7852
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8373..9164
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9410..9998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9899..9918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10152..10169
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10184..10324
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10250..10269)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10410..10432
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10668..10862
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10928..12001)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12430..13059)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    join(14390..14394,1..20)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 cccgccaata tatcctgtca aacactgata gttttgagac ttttcaacaa agggtaatat
       61 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag
      121 aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
      181 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
      241 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat ggtggagcac
      301 gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt
      361 gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc
      421 tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc
      481 gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc
      541 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
      601 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
      661 gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg aaaactagta
      721 tggatcatgc gaccctcgcc atgatcctcg cgatctggtt catcagcttc cacttcatca
      781 agctgctgtt ctcccagcag accaccaagc tgcttccgcc aggaccaaag ccgcttccga
      841 tcatcggcaa catccttgag gtgggcaaga agccgcatcg gtccttcgcc aacctcgcca
      901 agattcacgg cccactcatt tccctcagac tcggctctgt gaccaccatc gttgtgtcct
      961 ctgccgacgt ggccaaagag atgttcctca agaaggatca cccgctctcc aaccgcacga
     1021 tcccgaatag tgttacagcc ggcgaccacc acaagctcac catgtcttgg ctcccggtgt
     1081 ctccgaagtg gcgcaacttc cgcaagatta ccgccgtgca tctgctctcc ccacagagac
     1141 tcgatgcctg ccagacattc aggcacgcca aggtgcagca gctctacgag tacgttcaag
     1201 agtgcgccca gaaaggccag gccgtggata ttggcaaggc cgcctttacg accagcctca
     1261 acctcctcag caagctgttc ttcagcgtcg agctggcgca ccacaagtcc cataccagcc
     1321 aagagttcaa agagctgatc tggaacatca tggaagatat aggcaagccg aactacgccg
     1381 actacttccc gattctcggc tgcgttgacc catctggcat tagaagaagg ctcgcctgct
     1441 ccttcgacaa gctgatcgcc gtgttccagg gcatcatctg cgagagactc gccccagatt
     1501 cctccaccac aactaccacc accaccgacg acgtgctcga tgtgctcctc cagctgttca
     1561 agcagaacga gctgacgatg ggcgagatca accacctcct cgtggacatc ttcgacgccg
     1621 gcaccgatac cacatcctcc acattcgagt gggtgatgac cgagctgatc cgcaatccag
     1681 agatgatgga aaaggcccaa gaggaaatca agcaggtcct cggcaaggac aagcagatcc
     1741 aagagtccga catcatcaac ctgccgtacc tccaggcgat catcaaagag acactccgcc
     1801 tccatccgcc gaccgtgttc ttgctcccaa gaaaggccga caccgatgtc gagctgtacg
     1861 gctacatcgt gccgaaggat gcccagatcc tcgtgaacct ctgggccatt ggcagggacc
     1921 caaacgcctg gcagaacgcc gatattttca gcccagagcg cttcatcggc tgcgagatcg
     1981 atgttaaggg ccgcgatttc ggcctccttc catttggcgc tggccgcaga atttgcccag
     2041 gcatgaatct cgccatcagg atgctcaccc tcatgctcgc cacactcctc cagttcttca
     2101 actggaagct cgaaggcgac atctccccga aggacctcga catggacgag aagttcggca
     2161 ttgcgctcca aaagaccaag ccgctcaagc tcatcccgat tccgcgctac ggtagcggag
     2221 ctaccaattt tagcctcctt aagcaggcag gtgatgtaga agagaacccc gggcctatga
     2281 agatgatgaa cggcgaggac gccaacgacc agatgatcaa agagtccttc ttcatcaccc
     2341 acggcaaccc gatcctcacc gtcgaggata cacatccgct caggccgttc ttcgagacat
     2401 ggcgcgagaa gattttctcc aagaagccga aggccatcct catcatctcc ggccactggg
     2461 agacagtgaa gccaaccgtg aacgccgtgc acatcaacga caccatccac gacttcgacg
     2521 actacccagc cgccatgtac cagttcaagt acccagctcc aggcgagcca gagcttgcga
     2581 gaaaggtgga agagatcctc aagaagtccg ggttcgagac agccgagaca gaccaaaaga
     2641 ggggccttga tcacggcgcc tgggttccac tcatgctcat gtatccagag gcggacatcc
     2701 cggtgtgcca gctctcagtt cagccacatc tcgacggcac ctaccactac aatctcggca
     2761 gagccctcgc gccgctcaag aatgatggcg tgctcattat tggctccggc agcgccacac
     2821 atccactcga tgagacaccg cactacttcg atggtgttgc cccttgggcc gctgccttcg
     2881 attcttggct taggaaggcc ctcatcaacg gccgcttcga ggaagtgaac atctacgaga
     2941 gcaaggcccc gaactggaag ctcgcccatc catttccaga gcacttctac ccgctccacg
     3001 ttgtgctcgg cgctgctggt gaaaagtgga aggccgagct gatccactcc tcctgggatc
     3061 atggcacact ttgccacggc tcctacaagt tcacctccgc cggatccgga gcaaccaact
     3121 ttagcctgct caagcaagca ggagatgttg aggaaaatcc tggccccatg accgccatca
     3181 agatgaacac caacggcgag ggcgagacac agcacatcct catgatcccg ttcatggcgc
     3241 agggccacct caggccattt ctcgaactcg ccatgttcct ctacaagcgc tcccacgtga
     3301 tcatcaccct gctcacaact ccgctcaatg ccggcttcct caggcacctc cttcaccacc
     3361 attcctactc ctccagcggc atcaggatcg tcgagctgcc attcaactcc accaaccacg
     3421 gactcccacc gggcatcgag aacaccgata agctcacact cccgctcgtg gtgtccctct
     3481 tccattccac catcagcctc gatccgcacc tccgcgatta catctccagg catttcagcc
     3541 cagccaggcc accactctgc gtgatccatg atgtgttcct cggctgggtt gaccaggtgg
     3601 ccaaggatgt gggctctaca ggcgtggtgt tcacaacagg cggcgcttat ggcacatccg
     3661 cctacgtgtc catctggaac gatctcccgc accagaacta ctccgacgac caagagttcc
     3721 cgctgccagg cttcccagag aaccataagt tccgcaggtc ccagctccat cggttcctca
     3781 gatatgccga cggctccgac gattggtcca agtatttcca gccgcagctc cgccagtcca
     3841 tgaagtcttt tggctggctc tgcaactccg tggaagagat cgagacactc ggcttctcca
     3901 tcctccgcaa ctacaccaag ctgccgatct ggggcatcgg cccacttatt gcttccccag
     3961 tgcagcactc ctcctccgac aacaattcaa caggcgccga gttcgtgcag tggctcagcc
     4021 tcaaagagcc ggactccgtc ctctacatct ccttcggctc ccagaacacg atcagcccga
     4081 cgcagatgat ggaactcgct gctggccttg agtcctccga gaagccattc ctctgggtga
     4141 tcagagcccc gttcggcttc gacatcaacg aagagatgcg cccagagtgg ctgccagagg
     4201 gctttgagga acgcatgaag gtgaagaaac agggcaagct cgtgtacaag ctcggcccgc
     4261 agcttgagat cctcaaccat gaatccatcg gcggctttct cacccactgc ggatggaaca
     4321 gcatccttga gtctcttcgc gagggcgttc cgatgcttgg atggccactt gctgccgagc
     4381 aggcctacaa cctcaagtac ctcgaagatg agatgggcgt cgcggttgag cttgctagag
     4441 gcctcgaagg cgagatctcc aaagagaagg tcaagcgcat cgtcgagatg atccttgagc
     4501 gcaacgaggg ctccaaaggc tgggagatga agaatcgcgc cgtggaaatg ggcaaaaagc
     4561 tcaaggacgc cgtgaacgag gaaaaagagc tgaagggctc ctccgtgaag gcgatcgacg
     4621 atttcctcga cgccgtcatg caggccaaac ttgagccaag cctccagtga tagtgaatat
     4681 gaagatgaag atgaaatatt tggtgtgtca aataaaaagg ttgtgtgctt aagtttgtgt
     4741 ttttttcttg gcttgttgtg ttatgaattt gtggcttttt ctaatattaa atgaatgtaa
     4801 catctcatta taatgaataa acaaatgttt ctataatcca ttgtgaatgt tttgttggat
     4861 ctcttctcca gcatataact actgtatgtg ctatggtatg gactatggaa tatgattaaa
     4921 gataagacct aactgagtaa gctagcttgt tcgagtatta tggcattggg aaaactgttt
     4981 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg
     5041 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt
     5101 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa
     5161 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg
     5221 aagttaatat gaggagtaaa acactagatc cccaaacaag cttaaactga aggcgggaaa
     5281 cgacaatctg atcatgagcg gagaattaag ggagtcacgt tatgaccccc gccgatgacg
     5341 cgggacaagc cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactcag
     5401 ccgcgggttt ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt
     5461 caaaagtcgc ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg
     5521 acgttccata aattcccctc ggtatccaat tagagtctca tattcactct caatccaaat
     5581 aatctgcacc ggatctggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     5641 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5701 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5761 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     5821 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     5881 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     5941 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6001 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6061 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6121 cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc gtcgtgaccc atggcgatgc
     6181 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6241 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6301 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6361 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6421 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     6481 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6541 cgcggggatc tcatgctgga gttcttcgcc cacgggatct ctgcggaaca ggcggtcgaa
     6601 ggtgccgata tcattacgac agcaacggcc gacaagcaca acgccacgat cctgagcgac
     6661 aatatgatcg ggcccggcgt ccacatcaac ggcgtcggcg gcgactgccc aggcaagacc
     6721 gagatgcacc gcgatatctt gctgcgttcg gatattttcg tggagttccc gccacagacc
     6781 cggatgatcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     6841 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     6901 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     6961 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     7021 gcggtgtcat ctatgttact agatcgggcc tcctgtcaat gcgtcgacgc gtcaattgat
     7081 atcgatccag gcctcccagc tttcgtccgt atcatcggtt tcgacaacgt tcgtcaagtt
     7141 caatgcatca gtttcattgc ccacacacca gaatcctact aagtttgagt attatggcat
     7201 tggaaaagct gttttcttct atcatttgtt ctgcttgtaa tttactgtgt tctttcagtt
     7261 tttgttttcg gacatcaaaa tgcaaatgga tggataagag ttaataaatg atatggtcct
     7321 tttgttcatt ctcaaattat tattatctgt tgtttttact ttaatgggtt gaatttaagt
     7381 aagaaaggaa ctaacagtgt gatattaagg tgcaatgtta gacatataaa acagtctttc
     7441 acctctcttt ggttatgtct tgaattggtt tgtttcttca cttatctgtg taatcaagtt
     7501 tactatgagt ctatgatcaa gtaattatgc aatcaagtta agtacagtat aggctttttg
     7561 tgtcgagggg gtaccgagtc gaggaattca ctggccgtcg ttttacaacg tcgtgactgg
     7621 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     7681 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     7741 gggtaccgag ctcgaattca attcggcgtt aattcagtac attaaaaacg tccgcaatgt
     7801 gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag
     7861 ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc
     7921 agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc
     7981 gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg
     8041 agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca attcgggcac
     8101 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     8161 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     8221 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     8281 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     8341 agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat
     8401 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     8461 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     8521 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     8581 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     8641 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     8701 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     8761 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     8821 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     8881 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     8941 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     9001 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     9061 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     9121 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta gctagaaatt
     9181 cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg cactaagcac
     9241 ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt aagcgtgcat
     9301 aataagccct acacaaattg ggagatatat catgcatgac caaaatccct taacgtgagt
     9361 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9421 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9481 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9541 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9601 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9661 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9721 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9781 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9841 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9901 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9961 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10021 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10081 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10141 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
    10201 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
    10261 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
    10321 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
    10381 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
    10441 gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg
    10501 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
    10561 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
    10621 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
    10681 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
    10741 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
    10801 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
    10861 caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
    10921 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
    10981 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
    11041 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
    11101 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
    11161 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
    11221 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
    11281 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
    11341 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
    11401 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
    11461 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
    11521 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
    11581 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
    11641 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
    11701 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
    11761 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
    11821 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
    11881 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
    11941 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca
    12001 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga caacccagcc
    12061 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc
    12121 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta
    12181 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg
    12241 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa
    12301 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc
    12361 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac
    12421 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc
    12481 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta
    12541 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc
    12601 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg
    12661 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc
    12721 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc
    12781 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc
    12841 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg
    12901 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag
    12961 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc
    13021 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt
    13081 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat
    13141 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag
    13201 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg
    13261 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta
    13321 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt
    13381 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc
    13441 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg
    13501 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca
    13561 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc
    13621 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga
    13681 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg
    13741 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt
    13801 ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc
    13861 cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga
    13921 tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca
    13981 agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt
    14041 tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg
    14101 cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt
    14161 gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc
    14221 agcggagggg ttggatcaaa gtactttaaa gtactttaaa gtactttaaa gtactttgat
    14281 cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat aaaccttttc
    14341 acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg ttta
//
