LOCUS       sequence-488130-        7893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_106, derived from human fusion protein MEF2D_HNRNPUL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Maiti S, Tripathi S, Baggett DW, Phillips AH, Park CG, Wang J, 
            Wahiduzzaman, Freyaldenhoven WT, Kinger S, Pioso BJ, Bollinger JC, 
            Somjee R, Lang B, Babu MM, Kriwacki RW
  TITLE     Proteome-wide computational analyses reveal links between protein 
            condensate formation and RNA biology.
  JOURNAL   Sci Adv. 2025 Dec 5;11(49):eady1420. doi: 10.1126/sciadv.ady1420. 
            Epub 2025 Dec 3.
  PUBMED    41337585
REFERENCE   2  (bases 1 to 7893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-12-5"; volume: "11(49):eady1420. doi"; pages: " 
            10.1126/sciadv.ady1420. Epub 2025 Dec 3"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_106
FEATURES             Location/Qualifiers
     source          1..7893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1426..2136,2137..2139,2140..2142))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1468..1489)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1817..1836
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2076..2097
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2139..2148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2224..2427)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2253..2273)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2428..2731
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2988..3032)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3217..3450
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3810..3927
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4419..4544
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4591..4848
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4850..5229
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5260..5364
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5365..5433,5434..6225)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5583..5602)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6396..6984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6885..6904
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7138..7155
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7223..7357
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7247..7266)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7301..7320
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7363..7558)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7377..7512
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7617..7638
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7653..7658,7659..7676,7677..7683)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7691..7707
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7696..7718
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7715..7731
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7715..7731
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca ctgtgtgctc gtaccaccct gcgtgttccc
      601 gctataagat ccgtaatcgt agttgccgta gtatggcggc cattgccctt ggttttggta
      661 gtactgattc cattgttggg cgtactgctg atattgattg tatgagggtt gcgggtaggt
      721 ctgggcagtc gggggcgggg gaggcgtata gggcgctgga ttataaccgc cgtaggaacc
      781 gtaattgtat gcgggtggtg ggggcggagg tggcggggga gcggtataac cttgagaata
      841 tcctccttga ttatagggag gctggctata agaaggttga ggaggggaat aggatgaaac
      901 agttggagta gatgtattcg cacttgaacc aggtatgttg ctatttttat tgtacgtact
      961 ggctcctggt ggatttctcg ccggggaata tgagggtggc ggcgggggtt gttgcggtgg
     1021 cggttgaggc gggggaggct gttgaggcgg gggttgttgc tgaggtgctc gattgtagga
     1081 ccctcggttg tttgaattgt tattatccct gttgttattc ccccagcgat tttggctgta
     1141 tcctccaccc ccactcctat taaaccctcc ccggtaattc ccgccgccgc cggaacctcc
     1201 ccctcgattc tggaagccgc cccggttgcc gccaggcgga cctcgattct catacctttg
     1261 gaaccccccc cctccacccc tacctcggaa accaccaccg cctcggttgt caaaccgctt
     1321 ctccggagga gggccggcct ttcgcccctc ttcgttgtac tgccggacga gcttgtccgc
     1381 ctcccgggaa gcggccgcac ttcccccgga gcctccgctt ccgcccttgt acagctcgtc
     1441 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
     1501 ctcgttgggg tctttgctca gcttggactg ggtgctcagg tagtggttgt cgggcagcag
     1561 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
     1621 gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc
     1681 catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc
     1741 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
     1801 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
     1861 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
     1921 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
     1981 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
     2041 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
     2101 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggtggcga ccggtggatc
     2161 ccgggcccgc ggtaccgtcg actgcagaat tcggttcact aaacgagccc ggttcactaa
     2221 acgagctctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg
     2281 ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc
     2341 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc
     2401 attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta
     2461 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
     2521 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
     2581 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
     2641 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     2701 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
     2761 tgaccccgta attgattact attaataact agcacgcgtt cgaagcttga gctcggtccc
     2821 ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc
     2881 actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg
     2941 aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat
     3001 ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt
     3061 aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg
     3121 tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg
     3181 gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat
     3241 ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac
     3301 agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac
     3361 aataattgtc tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct
     3421 gctcccaaga acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc
     3481 actcttctct ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat
     3541 ttatataatt cacttctcca attgtccctc atatctcctc cttatggtgt tttactaaac
     3601 ttttccatgt tctaatcctc atcctgtcta cttgccacac aatcatcacc tgccatctgt
     3661 tttccataat ccctaatgat ctttgctttt cttcttggca ctacttttat gtcactatta
     3721 tcttgtatta ctactgcccc ttcacctttc cagaggagct ttgctggtcc tttccaaagt
     3781 ggatttctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt
     3841 aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac
     3901 cccccaatcc ccccttttct tttaaaattg tggatgaata ctgccatttg tactgctgtc
     3961 ttaagatgtt cagcctgatc tcttacctgt cctataattt tctttaattc tttattcata
     4021 gattctacta ctccttgact ttggggattg tagggaattt ccaggtctga agatcctgct
     4081 gcttgctgtg cttttttctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc
     4141 ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat
     4201 aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt
     4261 ctacagcctt ctgatgtttc taacaggcca gtattaactg cgaatcgttc tagctccctg
     4321 cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt
     4381 ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc
     4441 tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct
     4501 cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct
     4561 ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg
     4621 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
     4681 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
     4741 aggctcagat ctggtctaac cagagagacc cagtacaggc aaaaagcagc tgcttatatg
     4801 caggatctga gggctcgcca ctccccagtc ccgcccaggc cacgcctccc atggtaatag
     4861 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     4921 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     4981 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     5041 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     5101 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     5161 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata
     5221 atcaatgtcg acccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5281 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5341 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5401 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5461 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5521 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5581 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5641 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5701 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5761 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5821 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5881 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5941 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6001 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6061 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6121 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6181 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6241 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6301 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6361 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6421 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6481 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6541 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6601 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6661 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6721 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6781 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6841 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6901 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6961 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7021 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7081 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7141 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     7201 tggccgattc attaatgcag cggatccaga catgataaga tacattgatg agtttggaca
     7261 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     7321 tttatttgta accattataa gctgcaataa acaagttagc tttttgcaaa agcctaggcc
     7381 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     7441 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     7501 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcgg
     7561 atccgctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7621 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7681 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7741 gccaagcttg ggctgcaggt cgagggatct ccataagaga agagggacag ctatgactgg
     7801 gagtagtcag gagaggagga aaaatctggc tagtaaaaca tgtaaggaaa attttaggga
     7861 tgttaaagaa aaaaataaca caaaacaaaa tat
//
