LOCUS       sequence-488134-        7626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_111, derived from human fusion protein MTF1_SF3A3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Maiti S, Tripathi S, Baggett DW, Phillips AH, Park CG, Wang J, 
            Wahiduzzaman, Freyaldenhoven WT, Kinger S, Pioso BJ, Bollinger JC, 
            Somjee R, Lang B, Babu MM, Kriwacki RW
  TITLE     Proteome-wide computational analyses reveal links between protein 
            condensate formation and RNA biology.
  JOURNAL   Sci Adv. 2025 Dec 5;11(49):eady1420. doi: 10.1126/sciadv.ady1420. 
            Epub 2025 Dec 3.
  PUBMED    41337585
REFERENCE   2  (bases 1 to 7626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-12-5"; volume: "11(49):eady1420. doi"; pages: " 
            10.1126/sciadv.ady1420. Epub 2025 Dec 3"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_111
FEATURES             Location/Qualifiers
     source          1..7626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1159..1869,1870..1872,1873..1875))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1201..1222)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1550..1569
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1809..1830
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1872..1881
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1957..2160)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1986..2006)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2161..2464
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2721..2765)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2950..3183
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3543..3660
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4152..4277
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4324..4581
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4583..4962
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4993..5097
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5098..5166,5167..5958)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5316..5335)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6129..6717
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6618..6637
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6871..6888
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6956..7090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6980..6999)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7034..7053
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7096..7291)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7110..7245
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7350..7371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7386..7391,7392..7409,7410..7416)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7424..7440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7429..7451
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7448..7464
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7448..7464
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca tttcggatta tagatgatct cattctcttc
      601 gtcctcgctc tcagactcgg agatttgctc ttcttcctcc tcttcacgtt cctcgcctgt
      661 gcgtgcctgt ttacgctgca cattttcatg ggtcaagtga cgttgctcct ctttacgttt
      721 agtttctgga cattcggact gaagggtcag ggtagcgcct tcgatgttgc ggggcatcgg
      781 cgtactgcct gggttgatcg tcaggtgaat ctgatcaggt gaaataatgt gttgcacgta
      841 accttgtgac atagcttcat gatcgatcaa atggaaaccc tcctcccctc caaccaggaa
      901 tggaagatgc tctccacact gaccatcgtc gtcctcatct tccagggtac cggggtcctg
      961 ttcaataaga acagtagtgc ggtcgtagac tgttccgcta ctcgatggga caagaccatt
     1021 cttatccacg aaacgaagca tcttgtcatc tggagtaagt tcatcctctt ccgcttcaaa
     1081 gtaaatgata ttgttgtcag gagaatgctc tcccatccgg gaagcggccg cacttccccc
     1141 ggagcctccg cttccgccct tgtacagctc gtccatgccg agagtgatcc cggcggcggt
     1201 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga
     1261 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
     1321 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
     1381 gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta
     1441 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
     1501 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
     1561 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt
     1621 gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt
     1681 cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
     1741 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg
     1801 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
     1861 gcccttgctc accatggtgg cgaccggtgg atcccgggcc cgcggtaccg tcgactgcag
     1921 aattcggttc actaaacgag cccggttcac taaacgagct ctgcttatat agacctccca
     1981 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
     2041 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
     2101 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
     2161 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     2221 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     2281 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     2341 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     2401 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     2461 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     2521 actagcacgc gttcgaagct tgagctcggt cccctcgggg ttgggaggtg ggtctgaaac
     2581 gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt
     2641 cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt
     2701 gttatgttaa accaattcca caaacttgcc catttatcta attccaataa ttcttgttca
     2761 ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg
     2821 ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta
     2881 ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc
     2941 caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg
     3001 agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc
     3061 agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc
     3121 gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct
     3181 cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct
     3241 actcctaatg gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc
     3301 ctcatatctc ctccttatgg tgttttacta aacttttcca tgttctaatc ctcatcctgt
     3361 ctacttgcca cacaatcatc acctgccatc tgttttccat aatccctaat gatctttgct
     3421 tttcttcttg gcactacttt tatgtcacta ttatcttgta ttactactgc cccttcacct
     3481 ttccagagga gctttgctgg tcctttccaa agtggatttc tgctgtccct gtaataaacc
     3541 cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat gtctgttgct
     3601 attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt tcttttaaaa
     3661 ttgtggatga atactgccat ttgtactgct gtcttaagat gttcagcctg atctcttacc
     3721 tgtcctataa ttttctttaa ttctttattc atagattcta ctactccttg actttgggga
     3781 ttgtagggaa tttccaggtc tgaagatcct gctgcttgct gtgctttttt cttacttttg
     3841 ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga
     3901 tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc
     3961 tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg
     4021 ccagtattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta
     4081 tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc
     4141 cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt
     4201 tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga
     4261 gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc
     4321 cactgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
     4381 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
     4441 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
     4501 acccagtaca ggcaaaaagc agctgcttat atgcaggatc tgagggctcg ccactcccca
     4561 gtcccgccca ggccacgcct cccatggtaa tagcgatgac taatacgtag atgtactgcc
     4621 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     4681 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     4741 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     4801 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     4861 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     4921 ccgtaattga ttactattaa taactagtca ataatcaatg tcgacccagg tggcactttt
     4981 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5041 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5101 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5161 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5221 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5281 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5341 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5401 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5461 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5521 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5581 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5641 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5701 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5761 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5821 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5881 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5941 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6001 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6061 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6121 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6181 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6241 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6301 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6361 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6421 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6481 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6541 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6601 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6661 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6721 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6781 tttcctgcgt tatcccctgc ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     6841 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     6901 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcggatcc
     6961 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     7021 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     7081 taaacaagtt agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     7141 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     7201 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     7261 cgggactatg gttgctgact aattgagatg cggatccgct ggcacgacag gtttcccgac
     7321 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     7381 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     7441 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgggctgca ggtcgaggga
     7501 tctccataag agaagaggga cagctatgac tgggagtagt caggagagga ggaaaaatct
     7561 ggctagtaaa acatgtaagg aaaattttag ggatgttaaa gaaaaaaata acacaaaaca
     7621 aaatat
//
