LOCUS       sequence-488148-        7809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_125, derived from human fusion protein NUP98_NSD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Maiti S, Tripathi S, Baggett DW, Phillips AH, Park CG, Wang J, 
            Wahiduzzaman, Freyaldenhoven WT, Kinger S, Pioso BJ, Bollinger JC, 
            Somjee R, Lang B, Babu MM, Kriwacki RW
  TITLE     Proteome-wide computational analyses reveal links between protein 
            condensate formation and RNA biology.
  JOURNAL   Sci Adv. 2025 Dec 5;11(49):eady1420. doi: 10.1126/sciadv.ady1420. 
            Epub 2025 Dec 3.
  PUBMED    41337585
REFERENCE   2  (bases 1 to 7809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-12-5"; volume: "11(49):eady1420. doi"; pages: " 
            10.1126/sciadv.ady1420. Epub 2025 Dec 3"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_125
FEATURES             Location/Qualifiers
     source          1..7809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1342..2052,2053..2055,2056..2058))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1384..1405)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1733..1752
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1992..2013
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2055..2064
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2140..2343)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2169..2189)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2344..2647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2904..2948)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3133..3366
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3726..3843
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4335..4460
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4507..4764
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4766..5145
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5176..5280
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5281..5349,5350..6141)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5499..5518)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6312..6900
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6801..6820
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7054..7071
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7139..7273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7163..7182)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7217..7236
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7279..7474)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7293..7428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7533..7554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7569..7574,7575..7592,7593..7599)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7607..7623
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7612..7634
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7631..7647
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7631..7647
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca accgcgttca ccctgcattt ttttagaggc
      601 aggcatcccg ggatcgtgtt ctacaccttc ctcaacagtc tccttggggc ttgtggccgt
      661 cctgtgaggc attagttctc cctctgagcc tggaatctct tttgacgagt catcattttt
      721 cactttttta cactgcatct tggctgcagc atgcctctgt cgttttcgct tccttggctt
      781 gtcaaatatc ttggtagtgc ctccaccaaa atcttttgaa taagatgtgg cacaagattc
      841 agttatgcca ttctccaggt gaccagcttc atagcacttt ttagaaaggc caaggtcccc
      901 cttcttgggc ataaatccag ggtctaaatc attggattca agaagtttct tagttggttt
      961 tctttgacgt ttactttcat aatttcctgg cgttttaact agcaattcct cagactcaag
     1021 ggcaggccgt ggagagactt ccggagccac aggcacagac aaggtcagct gcggcaactc
     1081 agcatgtcca cctccaagtt ctgactgagc caaggggccc tccaaaaacg gagcctccct
     1141 ttcaagaact ggaggctctt ctttggttga aatcaaagaa ttctcgtcca cctgcttgtt
     1201 ctgagcacta gatcgacctc gggctagaag gctttcctct tcacagcggg aacttacctt
     1261 gtgcacctgc tcctgtacct tcttttgttt tttcttaggc cgggaagcgg ccgcacttcc
     1321 cccggagcct ccgcttccgc ccttgtacag ctcgtccatg ccgagagtga tcccggcggc
     1381 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt
     1441 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
     1501 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
     1561 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
     1621 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
     1681 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
     1741 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
     1801 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
     1861 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
     1921 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
     1981 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
     2041 ctcgcccttg ctcaccatgg tggcgaccgg tggatcccgg gcccgcggta ccgtcgactg
     2101 cagaattcgg ttcactaaac gagcccggtt cactaaacga gctctgctta tatagacctc
     2161 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
     2221 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     2281 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
     2341 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     2401 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     2461 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     2521 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     2581 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     2641 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     2701 ataactagca cgcgttcgaa gcttgagctc ggtcccctcg gggttgggag gtgggtctga
     2761 aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact
     2821 attcttaaac ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa
     2881 tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt
     2941 tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa
     3001 ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat
     3061 ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa
     3121 ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc
     3181 tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc
     3241 ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc
     3301 agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa
     3361 gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt
     3421 gctactccta atggttcaat ttttactact ttatatttat ataattcact tctccaattg
     3481 tccctcatat ctcctcctta tggtgtttta ctaaactttt ccatgttcta atcctcatcc
     3541 tgtctacttg ccacacaatc atcacctgcc atctgttttc cataatccct aatgatcttt
     3601 gcttttcttc ttggcactac ttttatgtca ctattatctt gtattactac tgccccttca
     3661 cctttccaga ggagctttgc tggtcctttc caaagtggat ttctgctgtc cctgtaataa
     3721 acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt
     3781 gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta
     3841 aaattgtgga tgaatactgc catttgtact gctgtcttaa gatgttcagc ctgatctctt
     3901 acctgtccta taattttctt taattcttta ttcatagatt ctactactcc ttgactttgg
     3961 ggattgtagg gaatttccag gtctgaagat cctgctgctt gctgtgcttt tttcttactt
     4021 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
     4081 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
     4141 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
     4201 aggccagtat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
     4261 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
     4321 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
     4381 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
     4441 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
     4501 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
     4561 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
     4621 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
     4681 gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc tcgccactcc
     4741 ccagtcccgc ccaggccacg cctcccatgg taatagcgat gactaatacg tagatgtact
     4801 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     4861 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     4921 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     4981 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     5041 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     5101 accccgtaat tgattactat taataactag tcaataatca atgtcgaccc aggtggcact
     5161 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5221 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5281 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5341 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5401 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5461 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5521 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5581 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5641 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5701 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5761 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5821 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5881 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5941 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6001 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6061 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6121 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6181 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6241 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6301 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6361 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6421 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6481 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6541 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6601 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6661 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6721 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6781 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6841 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6901 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6961 ttctttcctg cgttatcccc tgcttctgtg gataaccgta ttaccgcctt tgagtgagct
     7021 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7081 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcgga
     7141 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     7201 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     7261 caataaacaa gttagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     7321 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     7381 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     7441 gggcgggact atggttgctg actaattgag atgcggatcc gctggcacga caggtttccc
     7501 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7561 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7621 caatttcaca caggaaacag ctatgaccat gattacgcca agcttgggct gcaggtcgag
     7681 ggatctccat aagagaagag ggacagctat gactgggagt agtcaggaga ggaggaaaaa
     7741 tctggctagt aaaacatgta aggaaaattt tagggatgtt aaagaaaaaa ataacacaaa
     7801 acaaaatat
//
