LOCUS       sequence-488165-        7335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_144, derived from human fusion protein PAX5_NOL4L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Maiti S, Tripathi S, Baggett DW, Phillips AH, Park CG, Wang J, 
            Wahiduzzaman, Freyaldenhoven WT, Kinger S, Pioso BJ, Bollinger JC, 
            Somjee R, Lang B, Babu MM, Kriwacki RW
  TITLE     Proteome-wide computational analyses reveal links between protein 
            condensate formation and RNA biology.
  JOURNAL   Sci Adv. 2025 Dec 5;11(49):eady1420. doi: 10.1126/sciadv.ady1420. 
            Epub 2025 Dec 3.
  PUBMED    41337585
REFERENCE   2  (bases 1 to 7335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-12-5"; volume: "11(49):eady1420. doi"; pages: " 
            10.1126/sciadv.ady1420. Epub 2025 Dec 3"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_144
FEATURES             Location/Qualifiers
     source          1..7335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(868..1578,1579..1581,1582..1584))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(910..931)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1259..1278
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1518..1539
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1581..1590
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1666..1869)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1695..1715)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1870..2173
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2430..2474)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2659..2892
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3252..3369
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3861..3986
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4033..4290
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4292..4671
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4702..4806
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4807..4875,4876..5667)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5025..5044)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5838..6426
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6327..6346
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6580..6597
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6665..6799
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6689..6708)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6743..6762
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(6805..7000)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6819..6954
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7059..7080
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7095..7100,7101..7118,7119..7125)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7133..7149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7138..7160
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7157..7173
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7157..7173
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca gtcttgtgaa tacgaactgg ccggaagaga
      601 atacgtgctg tgtgtgattg cggcagaatc gcgagaatgt tgcttatcca gcgcgattgg
      661 ttcgtcctgc gatgattggt agatttccag acgcatacgt ttcgcagctt tgcgagtttc
      721 ggactcgcag gccgcggcca aaatattttc tgccatcgcg gaggttaaat gcggtggagt
      781 agggcgggtc atctccatgc cattcttctt catacggcga caactccggg aagcggccgc
      841 acttcccccg gagcctccgc ttccgccctt gtacagctcg tccatgccga gagtgatccc
      901 ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct
      961 cagcttggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
     1021 gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg
     1081 gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg
     1141 gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
     1201 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
     1261 cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat
     1321 ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac
     1381 gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca
     1441 gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct
     1501 gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa
     1561 cagctcctcg cccttgctca ccatggtggc gaccggtgga tcccgggccc gcggtaccgt
     1621 cgactgcaga attcggttca ctaaacgagc ccggttcact aaacgagctc tgcttatata
     1681 gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca
     1741 ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg
     1801 gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa
     1861 ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
     1921 ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat
     1981 agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa
     2041 atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt
     2101 cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt
     2161 aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta
     2221 ctattaataa ctagcacgcg ttcgaagctt gagctcggtc ccctcggggt tgggaggtgg
     2281 gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga aagtacagca
     2341 aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt tatataccac
     2401 agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat
     2461 tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt
     2521 gtgtaattgt taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc
     2581 agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca
     2641 gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt
     2701 cttgcctgga gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca
     2761 atagccctca gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt
     2821 accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg
     2881 aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg
     2941 gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc
     3001 caattgtccc tcatatctcc tccttatggt gttttactaa acttttccat gttctaatcc
     3061 tcatcctgtc tacttgccac acaatcatca cctgccatct gttttccata atccctaatg
     3121 atctttgctt ttcttcttgg cactactttt atgtcactat tatcttgtat tactactgcc
     3181 ccttcacctt tccagaggag ctttgctggt cctttccaaa gtggatttct gctgtccctg
     3241 taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg
     3301 tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt
     3361 cttttaaaat tgtggatgaa tactgccatt tgtactgctg tcttaagatg ttcagcctga
     3421 tctcttacct gtcctataat tttctttaat tctttattca tagattctac tactccttga
     3481 ctttggggat tgtagggaat ttccaggtct gaagatcctg ctgcttgctg tgcttttttc
     3541 ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct
     3601 atcctttgat gcacacaata gagggttgct actgtattat ataatgatct aagttcttct
     3661 gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt
     3721 tctaacaggc cagtattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt
     3781 tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta
     3841 attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta
     3901 gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca
     3961 gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg
     4021 ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
     4081 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
     4141 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
     4201 accagagaga cccagtacag gcaaaaagca gctgcttata tgcaggatct gagggctcgc
     4261 cactccccag tcccgcccag gccacgcctc ccatggtaat agcgatgact aatacgtaga
     4321 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
     4381 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
     4441 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     4501 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     4561 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
     4621 ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt cgacccaggt
     4681 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4741 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4801 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4861 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4921 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4981 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5041 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5101 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5161 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5221 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5281 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5341 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5401 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5461 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5521 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5581 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5641 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5701 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5761 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5821 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5881 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5941 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6001 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6061 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6121 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6181 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6241 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6301 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6361 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6421 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6481 cacatgttct ttcctgcgtt atcccctgct tctgtggata accgtattac cgcctttgag
     6541 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6601 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6661 agcggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     6721 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     6781 aagctgcaat aaacaagtta gctttttgca aaagcctagg cctccaaaaa agcctcctca
     6841 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
     6901 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     6961 agttaggggc gggactatgg ttgctgacta attgagatgc ggatccgctg gcacgacagg
     7021 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     7081 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     7141 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgggctgcag
     7201 gtcgagggat ctccataaga gaagagggac agctatgact gggagtagtc aggagaggag
     7261 gaaaaatctg gctagtaaaa catgtaagga aaattttagg gatgttaaag aaaaaaataa
     7321 cacaaaacaa aatat
//
