LOCUS       sequence-488199-        7431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_179, derived from human fusion protein SMARCC1_MAP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Maiti S, Tripathi S, Baggett DW, Phillips AH, Park CG, Wang J, 
            Wahiduzzaman, Freyaldenhoven WT, Kinger S, Pioso BJ, Bollinger JC, 
            Somjee R, Lang B, Babu MM, Kriwacki RW
  TITLE     Proteome-wide computational analyses reveal links between protein 
            condensate formation and RNA biology.
  JOURNAL   Sci Adv. 2025 Dec 5;11(49):eady1420. doi: 10.1126/sciadv.ady1420. 
            Epub 2025 Dec 3.
  PUBMED    41337585
REFERENCE   2  (bases 1 to 7431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-12-5"; volume: "11(49):eady1420. doi"; pages: " 
            10.1126/sciadv.ady1420. Epub 2025 Dec 3"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_179
FEATURES             Location/Qualifiers
     source          1..7431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(964..1674,1675..1677,1678..1680))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1006..1027)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1355..1374
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1614..1635
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1677..1686
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1762..1965)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1791..1811)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1966..2269
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2526..2570)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2755..2988
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3348..3465
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3957..4082
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4129..4386
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4388..4767
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4798..4902
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4903..4971,4972..5763)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5121..5140)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5934..6522
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6423..6442
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6676..6693
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6761..6895
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6785..6804)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6839..6858
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(6901..7096)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6915..7050
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7155..7176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7191..7196,7197..7214,7215..7221)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7229..7245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7234..7256
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7253..7269
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7253..7269
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca gatggaagtt tcctggatct gcgaatcaag
      601 cgtctgagct tcgcgctgat cacctccgcc tgataaggta ggatgaccat tcaaaccgct
      661 ggccgaagtg ggagcaccag cttcgggagc agcctccgag atagctggtt cttccccggc
      721 aggtggccca gggcacaaag gtgcctcgct accgccccct tccgttttca cagccccccc
      781 tgcgggcagg tgtccaacat tatccagaga accgaccttg gcctgagctt tctccttgaa
      841 gttcaatttc tgactttcaa ttttcacgtc tccaccacct ggcttgtgct taatgttagc
      901 tttggagcca cacttgctac tccgggaagc ggccgcactt cccccggagc ctccgcttcc
      961 gcccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
     1021 gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg tgctcaggta
     1081 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
     1141 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc
     1201 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
     1261 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
     1321 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
     1381 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
     1441 cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag
     1501 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
     1561 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
     1621 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
     1681 ggtggcgacc ggtggatccc gggcccgcgg taccgtcgac tgcagaattc ggttcactaa
     1741 acgagcccgg ttcactaaac gagctctgct tatatagacc tcccaccgta cacgcctacc
     1801 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
     1861 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
     1921 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
     1981 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
     2041 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
     2101 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
     2161 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
     2221 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
     2281 atatgggcta tgaactaatg accccgtaat tgattactat taataactag cacgcgttcg
     2341 aagcttgagc tcggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat
     2401 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag
     2461 cctcctacta tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa
     2521 ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg
     2581 ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc
     2641 cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca
     2701 ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc
     2761 tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc
     2821 cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc
     2881 tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg
     2941 cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct
     3001 cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca
     3061 atttttacta ctttatattt atataattca cttctccaat tgtccctcat atctcctcct
     3121 tatggtgttt tactaaactt ttccatgttc taatcctcat cctgtctact tgccacacaa
     3181 tcatcacctg ccatctgttt tccataatcc ctaatgatct ttgcttttct tcttggcact
     3241 acttttatgt cactattatc ttgtattact actgcccctt cacctttcca gaggagcttt
     3301 gctggtcctt tccaaagtgg atttctgctg tccctgtaat aaacccgaaa attttgaatt
     3361 tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt
     3421 ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg gatgaatact
     3481 gccatttgta ctgctgtctt aagatgttca gcctgatctc ttacctgtcc tataattttc
     3541 tttaattctt tattcataga ttctactact ccttgacttt ggggattgta gggaatttcc
     3601 aggtctgaag atcctgctgc ttgctgtgct tttttcttac ttttgttttg ctcttcctct
     3661 atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg
     3721 gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt
     3781 agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagt attaactgcg
     3841 aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt tttctttccc
     3901 cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg
     3961 ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc
     4021 agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag
     4081 agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt
     4141 tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg
     4201 cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta
     4261 gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa
     4321 aaagcagctg cttatatgca ggatctgagg gctcgccact ccccagtccc gcccaggcca
     4381 cgcctcccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     4441 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     4501 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     4561 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     4621 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     4681 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     4741 attaataact agtcaataat caatgtcgac ccaggtggca cttttcgggg aaatgtgcgc
     4801 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4861 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4921 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4981 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5041 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5101 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5161 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5221 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5281 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5341 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5401 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5461 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5521 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5581 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5641 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5701 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5761 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5821 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5881 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5941 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6001 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6061 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6121 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6181 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6241 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6301 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6361 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6421 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6481 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6541 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6601 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6661 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6721 ccgcctctcc ccgcgcgttg gccgattcat taatgcagcg gatccagaca tgataagata
     6781 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     6841 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttagctt
     6901 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     6961 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     7021 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     7081 tgactaattg agatgcggat ccgctggcac gacaggtttc ccgactggaa agcgggcagt
     7141 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7201 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     7261 agctatgacc atgattacgc caagcttggg ctgcaggtcg agggatctcc ataagagaag
     7321 agggacagct atgactggga gtagtcagga gaggaggaaa aatctggcta gtaaaacatg
     7381 taaggaaaat tttagggatg ttaaagaaaa aaataacaca aaacaaaata t
//
