LOCUS       sequence-48822-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Ma Y, Erkner A, Gong R, Yao S, Taipale J, Basler K, Beachy PA
  TITLE     Hedgehog-mediated patterning of the mammalian embryo requires 
            transporter-like function of dispatched.
  JOURNAL   Cell. 2002 Oct 4;111(1):63-75.
  PUBMED    12372301
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2002-10-4"; volume: "111(1)"; pages: "63-75"
COMMENT     Sequence Label: pcDNA3-ShhN-Ren
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(681..700)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(682..700)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(745..796)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(774..794)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aaactttcga agtcatcgct gcggcagccg ctgcacgcgt tttggccgcc acggagttct
       61 ctgctttcac agaacagtgg atgtgagctt tggattcata gtagacccag tcgaaacctg
      121 cttccacagc caggcgagcc agcatgccgt acttgctgcg gtcccggtcg gacgtggtga
      181 tgtccactgc tcgaccctca tagtgtagag actcctctga atgatggccg tcctcatccc
      241 agccctcggt cactcgcagc ttcactccag gccactggtt catcacagag atggccaagg
      301 catttaactt gtctttgcac ctctgagtca tcagccggtc tgctcccgtg ttttcctcat
      361 ccttaaatat gatgtcgggg ttgtaattgg gggtgagttc cttaaatcgt tcggagtttc
      421 ttgtgatctt cccttcatat ctgccgctgg cccctagggt cttctcagct acgttgggaa
      481 taaactgctt gtaggctaaa ggggtcagct ttttggggtg ccgcctcttt ccaaaccccc
      541 tgccgggccc acaggccagc ccggggcaca ccagcagcga ggaagcaagg atcaccagaa
      601 aacatctggc cagcagcagc agcatggtgg atccgagctc ggtaccaagc ttaagtttaa
      661 acgctagcca gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc
      721 agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct
      781 accgcccatt tgcgtcaatg gggcgga
//
