LOCUS       sequence-48825-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  TITLE     Beachy lab unpub
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1 ShhN
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(808..825)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccgagc tcggatccac
      121 tagtccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgcc accatgctgc
      181 tgctgctggc cagatgtttt ctggtgatcc ttgcttcctc gctgctggtg tgccccgggc
      241 tggcctgtgg gcccggcagg gggtttggaa agaggcggca ccccaaaaag ctgacccctt
      301 tagcctacaa gcagtttatt cccaacgtag ctgagaagac cctaggggcc agcggcagat
      361 atgaagggaa gatcacaaga aactccgaac gatttaagga actcaccccc aattacaacc
      421 ccgacatcat atttaaggat gaggaaaaca cgggagcaga ccggctgatg actcagaggt
      481 gcaaagacaa gttaaatgcc ttggccatct ctgtgatgaa ccagtggcct ggagtgaagc
      541 tgcgagtgac cgagggctgg gatgaggacg gccatcattc agaggagtct ctacactatg
      601 agggtcgagc agtggacatc accacgtccg accgggaccg cagcaagtac ggcatgctgg
      661 ctcgcctggc tgtggaagca ggtttcgact gggtctacta tgaatccaaa gctcacatcc
      721 actgttctgt gaaagcagag aactccgtgg cggccaaatc cggcggctga ctcgagtcta
      781 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
      841 ttgttt
//
