LOCUS       sequence-488264-       11529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein BRD9_NUTM1 with an IDR deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11529)
  AUTHORS   Maiti S, Tripathi S, Baggett DW, Phillips AH, Park CG, Wang J, 
            Wahiduzzaman, Freyaldenhoven WT, Kinger S, Pioso BJ, Bollinger JC, 
            Somjee R, Lang B, Babu MM, Kriwacki RW
  TITLE     Proteome-wide computational analyses reveal links between protein 
            condensate formation and RNA biology.
  JOURNAL   Sci Adv. 2025 Dec 5;11(49):eady1420. doi: 10.1126/sciadv.ady1420. 
            Epub 2025 Dec 3.
  PUBMED    41337585
REFERENCE   2  (bases 1 to 11529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-12-5"; volume: "11(49):eady1420. doi"; pages: " 
            10.1126/sciadv.ady1420. Epub 2025 Dec 3"
COMMENT     Sequence Label: CL20_mEGFP_BRD9_NUTM1-Delta-1-152
FEATURES             Location/Qualifiers
     source          1..11529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(4169..4180)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(join(5062..5772,5773..5775,5776..5778))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5104..5125)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5453..5472
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5712..5733
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5775..5784
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5860..6063)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5889..5909)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6064..6367
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6624..6668)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6853..7086
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7446..7563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8055..8180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8227..8484
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        8486..8865
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8896..9000
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9001..9069,9070..9861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9219..9238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10032..10620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10521..10540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10774..10791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    10859..10993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10883..10902)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10937..10956
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(10999..11194)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11013..11148
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    11253..11274
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11289..11294,11295..11312,11313..11319)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11327..11343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11332..11354
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11351..11367
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11351..11367
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggtcta ctggctacga cgtcgtttct tccttctgcc
      601 cgtgacaaaa ctgtcacacc gccttttacg aggctgtgag ggtcgaacta ctcccagagc
      661 taggggtttc tccctgggga ccccaagttg agctcctgag tggggtgtct tttcagtagg
      721 ggctggacct ccagctagag ctcgcttccc agacttggca acaggatatg gaagtttgct
      781 ggggcctttt gctccagcag ggagcaggtg ggatgctctc tggctgccct gacctcctga
      841 ggcatggtga ggactgaggg gatgctccct tggtgagagg ctaagtttag aggccaagag
      901 gtaagcaaag ttggagagtt cctcatcctc ttcctctgct tcctttttct ttttctcctt
      961 tcctttggcc ctgtctgctg acctgtgtgt tttactaaca gaagtttctc tcggaactat
     1021 ggcattcctg gttccccttc taggcaaagt gcttccaagg ccctggtgag aagatgttgc
     1081 tttgggagtc ccagttgtgt aagactcctg actctcttgt aaaggagcaa ggtttttagg
     1141 cttggacaga tcaggatcca ccctgccttc tccttggggg tcataagaat gaacattcag
     1201 tgggcaggtg tcctcgctga cccttagttg gaggccacag tcatctttaa catctagtat
     1261 gttgacaggt tctatggttt ccaacagagg agaaaaggaa ttgcctctgc tccctgcctc
     1321 ttgactggct tcaggcaagg aggaggcatc ctcagtcccc atgataagga gattgccttg
     1381 gcatgtgggt ggaagggttt ctttggaaga ccccagtgtg gaatcaccac tttctagcaa
     1441 tgggaagcaa ccttctgccc acagatcact gggatgccct acggtttcac agctctgttc
     1501 ttgattttct ttggacctca agggtgggct gttggccctc aaaggtcctg gcaagctgca
     1561 atagtttctc ttttctaggg cagctgccgc aactgtgcct ccacagggaa tcacactgct
     1621 ctccgtatcc ccaggtacta gggtttctcc aggacccagg gaaatgttgc ccctggagcc
     1681 cagtccctgg cagccttcct gatactcagt tacacacttc tcaacttgga agctctctat
     1741 gacctcctct atttgtacag gcagctccaa gcctacagca tccatctcac tgctcagcca
     1801 aactcctggg ctgagacaga catcgtccct ctccccagct gctctagggg aaggattctg
     1861 atcataactc tgagccatgg gggtacctct gtcatctccc cacatggctc ctgaagagcc
     1921 ttcccagggc actgctaaag gctccttccc ttgaggaagc actccacgac cccctgtttg
     1981 ttgtcctttc tgcaatccca ggacttgctt ttctaaccct tgtccttgca gaggagccag
     2041 ctctgcaagt ccagcatcca aactgggagt gctctcaggc tggaagcctc cctgccaaca
     2101 aaggggcaga gcctctgaag tcctatcagc caccaggcag tgcccaggag gcccagcgcc
     2161 tcctagatgg ccatcctgat gtagagatac ctggtttata accttcccag accctctcct
     2221 ctccacaccc aagggtcccg gagttccctg tggagctgca agttctggct gcaggtccca
     2281 gctgctgggg gatggcagag tgttcccatc cctgtgcatc cctctggggc tatctagggc
     2341 ttgctcctgc ccaccatgca cttctcttgc ccgttttcct gaagaagaaa cctttccaag
     2401 gcaagcggcg cccccagccc cctgaagccc aggtgagggc cgaagccgcc catccccatc
     2461 ttcatcctca acagaaccag aaggacttga gtccaactga gcgccactga aacttggagg
     2521 cgcctctgca tcttcctcct cttccaaggc catgagtcgc ttctggacca gctgggcaag
     2581 agtgagtcct tcttcttgct ctagctcctc aattaaggcc aagggatctc tctgtttttc
     2641 tggggacagc agatctgcca gaaattgagg gtgaatgaca gcctccacct tggagacaaa
     2701 gaccttctga gaacacagct cattgatgta gctcaggaga cctggatctg gatacatccc
     2761 ttcctcctcc tgctgctgcc cttcttcctc ttgttttcca tctgactccc cagtggccaa
     2821 gtgagtcccc accagccatt ccatgatgtc aacatactcc ttcacagctt ctggtgggat
     2881 ctccttgggt gcctcaggag caggaggtct ctgggcttta cgctgacgcc ggcggggggc
     2941 ccgtgtcttg gaggctgcct tcttcggaat gtacactggc tgctggcaaa cctcagaggc
     3001 caggggccct agaggatcaa gtttcaaagg ggttgcagga gacaggccct gagacccatt
     3061 catcagctgt gtgttctgaa tctgcatctc ctcagcctca aactccatga acctttctgc
     3121 catctcataa aagatcatcc ggtcaaagtt gctggtgtgc tcccactcct gcacagccaa
     3181 tggcagtccc tcctccaggg tcatagtggg cttcagccgg gccagggaac gaagcactgg
     3241 gataagaaaa caggaaagag cttctgtgtc aggactctgg gataggtgcc tccgggccaa
     3301 ggctttgtaa cgctgccact gacggaagtt ctcataaacg tccttggaaa ttttggagcg
     3361 gtcacctagg gaaggcttgg atagagtggc cacaggacct ccttccccgg ttgtcccatg
     3421 tggccctggc caagcttttt ccaggggcac aatggggacc agttgagcaa ctggtggtgg
     3481 aggctgaggc ggaaggcctg gaggaccctc ctggctgaca ccaacagcct tagagggcag
     3541 aatggtcttc acattagatg ctgtcacaaa tggaggtgca ggaccctcaa ggcctccaca
     3601 gggagtgccc ggggcagtcg aattgagggc agtctgagta aggataaagt tctgagtttg
     3661 agagggctca gctgaccccc cttctgtctt gactttgaca atgaccttgc cagccccagc
     3721 cccactgagg caagggcccc cgtcccctgt caccaacagt gagctgggga aagcagagag
     3781 catcagaggg ttgtctggag agaatactga aggcatgatg ggctgtggag gtggctccct
     3841 gggtgggtgg tctggtgggt cagaagttgg tgggagaaag ggaagtgcag gggatggaga
     3901 cagggcggca ctaggtttca tgctcatatc cggtcccggc aatgcagatg ccagagagct
     3961 gagcatggag atatccacag aaacgtcggg ataggacttc atcgacatga actccagaac
     4021 agagctgctg ctgtctccta gggtgtcccc aaccttggct tcatctggag gcttcatggg
     4081 aacatttctt ctctgcttca gctggaagag cgtcctagag tggtctccgc ctgtgatctg
     4141 gtccaggagg tcgtccacca ctttcttgct gtagctccca gcatccttca caaactcctg
     4201 caggctcagc gcacactgca cgcctgtctc atctccgtag gctgagtaga gcagctccat
     4261 ctcgtccgac ttcaagtcgc caaatactga attattctgc atcgaaagcg cagtagtggc
     4321 actggagaga aaggtgactt tgtttcttct ctcgtctttg aagcccagcg tggtgaagcc
     4381 tgggagtagc ttactggaga gcgagctcaa gtccaccggg tgggtctcct cctcatcagc
     4441 gtccggctcg gccgtgttga ccacgctgta gagcaggctc ccgtccccgt tcctcttcag
     4501 atagcccatc ttgccgcctg ggaggaaccg gttgatcctg tcccgagctt cgtcagctgc
     4561 gtgctccacc agcgccagca cgtgctcctc tgcggtactg tccgtcaagc tgcaggcatt
     4621 cccttcaggc tcaaacatgc agctgataac ttctctactc ggctttttgg atttcttggc
     4681 agtttctact tgtactggta caacttcagg gacaggttcc tcaacagctg tatcttcatt
     4741 gcccaaaaga gctgcctgtt tgctcatcat cttaaagcct gcgtgaagga tcttcttcgc
     4801 caacttgtag tacacggtat ctggcctatt gtatgtcatt gcattatcac acatcagctt
     4861 gaaatctgcc ttaaattccg taactgactt gtattcatta gctacaattt tgtctttcat
     4921 ggtgccaaaa tccatgggat gttttattat cattgaatat ccaggagcaa ttgcatccgt
     4981 gacaggaaaa gcaaaaaatc catggggatc ttttctctgc cgggaagcgg ccgcacttcc
     5041 cccggagcct ccgcttccgc ccttgtacag ctcgtccatg ccgagagtga tcccggcggc
     5101 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt
     5161 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
     5221 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
     5281 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
     5341 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
     5401 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
     5461 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
     5521 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
     5581 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
     5641 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
     5701 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
     5761 ctcgcccttg ctcaccatgg tggcgaccgg tggatcccgg gcccgcggta ccgtcgactg
     5821 cagaattcgg ttcactaaac gagcccggtt cactaaacga gctctgctta tatagacctc
     5881 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
     5941 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     6001 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
     6061 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     6121 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     6181 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     6241 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     6301 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     6361 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     6421 ataactagca cgcgttcgaa gcttgagctc ggtcccctcg gggttgggag gtgggtctga
     6481 aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact
     6541 attcttaaac ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa
     6601 tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt
     6661 tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa
     6721 ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat
     6781 ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa
     6841 ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc
     6901 tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc
     6961 ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc
     7021 agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa
     7081 gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt
     7141 gctactccta atggttcaat ttttactact ttatatttat ataattcact tctccaattg
     7201 tccctcatat ctcctcctta tggtgtttta ctaaactttt ccatgttcta atcctcatcc
     7261 tgtctacttg ccacacaatc atcacctgcc atctgttttc cataatccct aatgatcttt
     7321 gcttttcttc ttggcactac ttttatgtca ctattatctt gtattactac tgccccttca
     7381 cctttccaga ggagctttgc tggtcctttc caaagtggat ttctgctgtc cctgtaataa
     7441 acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt
     7501 gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta
     7561 aaattgtgga tgaatactgc catttgtact gctgtcttaa gatgttcagc ctgatctctt
     7621 acctgtccta taattttctt taattcttta ttcatagatt ctactactcc ttgactttgg
     7681 ggattgtagg gaatttccag gtctgaagat cctgctgctt gctgtgcttt tttcttactt
     7741 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
     7801 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
     7861 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
     7921 aggccagtat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
     7981 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
     8041 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
     8101 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
     8161 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
     8221 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
     8281 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
     8341 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
     8401 gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc tcgccactcc
     8461 ccagtcccgc ccaggccacg cctcccatgg taatagcgat gactaatacg tagatgtact
     8521 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     8581 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     8641 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     8701 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     8761 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     8821 accccgtaat tgattactat taataactag tcaataatca atgtcgaccc aggtggcact
     8881 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     8941 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     9001 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     9061 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     9121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     9181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     9241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     9301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     9361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     9421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     9481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     9541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     9601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     9721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     9781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     9961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    10021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    10081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    10141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    10201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    10261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    10321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    10381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    10441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    10501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    10561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    10621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    10681 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    10741 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
    10801 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcgga
    10861 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
    10921 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
    10981 caataaacaa gttagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
    11041 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
    11101 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
    11161 gggcgggact atggttgctg actaattgag atgcggatcc gctggcacga caggtttccc
    11221 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    11281 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
    11341 caatttcaca caggaaacag ctatgaccat gattacgcca agcttgggct gcaggtcgag
    11401 ggatctccat aagagaagag ggacagctat gactgggagt agtcaggaga ggaggaaaaa
    11461 tctggctagt aaaacatgta aggaaaattt tagggatgtt aaagaaaaaa ataacacaaa
    11521 acaaaatat
//
