LOCUS       sequence-488265-       12759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein CUX1_NUTM1 with an IDR deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12759)
  AUTHORS   Maiti S, Tripathi S, Baggett DW, Phillips AH, Park CG, Wang J, 
            Wahiduzzaman, Freyaldenhoven WT, Kinger S, Pioso BJ, Bollinger JC, 
            Somjee R, Lang B, Babu MM, Kriwacki RW
  TITLE     Proteome-wide computational analyses reveal links between protein 
            condensate formation and RNA biology.
  JOURNAL   Sci Adv. 2025 Dec 5;11(49):eady1420. doi: 10.1126/sciadv.ady1420. 
            Epub 2025 Dec 3.
  PUBMED    41337585
REFERENCE   2  (bases 1 to 12759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-12-5"; volume: "11(49):eady1420. doi"; pages: " 
            10.1126/sciadv.ady1420. Epub 2025 Dec 3"
COMMENT     Sequence Label: CL20_mEGFP_CUX1_NUTM1-Delta-986-1290
FEATURES             Location/Qualifiers
     source          1..12759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    828..962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(852..871)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     906..925
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(968..1163)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      982..1117
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    1222..1243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1258..1263,1264..1281,1282..1288)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1296..1312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1301..1323
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1320..1336
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1320..1336
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1679..1698
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1697..1752
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1798..1817
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(7790..8500,8501..8503,8504..8506))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7832..7853)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8181..8200
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8440..8461
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8503..8512
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8588..8791)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8617..8637)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8792..9095
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(9352..9396)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9581..9814
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10174..10291
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    10783..10908
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10955..11212
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        11214..11593
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11624..11728
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11729..11797,11798..12589)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11947..11966)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcggat ccagacatga
      841 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      901 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      961 ttagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     1021 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     1081 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     1141 tggttgctga ctaattgaga tgcggatccg ctggcacgac aggtttcccg actggaaagc
     1201 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     1261 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     1321 aggaaacagc tatgaccatg attacgccaa gcttgggctg caggtcgagg gatctccata
     1381 agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat ctggctagta
     1441 aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa caaaatataa
     1501 aaaaaatcta acctcaagtc aaggcttttc tatggaataa ggaatggaca gcagggggct
     1561 gtttcatata ctgatgacct ctttatagcc aacctttgtt catggcagcc agcatatggg
     1621 catatgttgc caaactctaa accaaatact cattctgatg ttttaaatga tttgccctcc
     1681 catatgtcct tccgagtgag agacacaaaa aattccaaca cactattgca atgaaaataa
     1741 atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc ccataatttt
     1801 tggcagaggg aaaaagatct gctagctcga gcagctgaag ctttattgag gcttaagcag
     1861 tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc
     1921 agtacaggca aaaagcagat cttgtcttct ttgggagtga attagccctt ccagtccccc
     1981 cttttctttt aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg
     2041 taccgtaatc ttatctagaa ccggttcatt ggctacggcg acgcttcttg cgacggcccg
     2101 tcacgaagct atcacaacgg cgtttacgag gctgactagg acgcacaaca ccgagtgcca
     2161 gcggtttctc tctcggaaca cccagttgcg caccgctatg cggggtcttt tcagtaggcg
     2221 ctggaccgcc agccaatgca cgtttgccgc tcttcgccac cgggtacggc aattttgacg
     2281 gacccttggc gcccgccggt aacaggtggg aggccctctg gctgccctga ccaccgcttg
     2341 catgatgcgg gctcagcggg tgctcacgcg gactgaggga cagcttgctc gccagcaggt
     2401 acgcgaagtt ggacagttcc tcgtcctcct cttccgcttc cttctttttc ttctccttgc
     2461 ctttcgcacg atcagcggaa cggtgcgttt tgctgacgct ggtctctcgg ggcacaatcg
     2521 cgttgcgggt gcctcggcgg gggagcgtag agcccaatcc ctggtgggaa gacgtcgctt
     2581 ttggagtccc ggtggtatag ctttcctgtg actcctgcag cggtgccaga ttcttcggtt
     2641 tggacagatc cgggtccacc ctgccctcac cctgcgggtc gtaggaatgc acgttcagcg
     2701 gacaggtgtc ttcgctaacg cgaagctgaa gaccacagtc gtctttaacg tccaggatat
     2761 taaccggttc aatcgtttct aacagagggc tgaaggaatt acctcgtgat cccgcttctt
     2821 ggctagcctc aggcaagctg ctcgcgtcct cagtacccat gatcagcaga ttgccctggc
     2881 acgtaggagg cagagtctct ttgctggaac ccagggtaga gtcgcctgat tccagcagcg
     2941 gaaagcagcc ctctgcccac agatcggacg gatgacccac tgtttcgcaa ctttgttcct
     3001 ggttctcctt agaacgtagc ggtgggctgt tcgcgcgcag gggccccggt aagctgcagt
     3061 aattacgttt ctccaaggca gcagccgcca cggtaccacc gcacgggatc acggatgatt
     3121 cggtgtcgcc agggaccagg gtctcacctg ggcccaggct aatattacca cggctgccca
     3181 aaccctggca gccctcctgg tactcggtca cgcacttctc cacctggaag ctctcgataa
     3241 cttcttcgat ctgaacggga agctccagac ctaccgcatc catctcgcta gacagccaaa
     3301 cgcccgggct cagacagaca tcatcgcgct cgcctgcggc tctggggctc gggttctgat
     3361 cgtaagattg cgccatcgga gtaccacggt cgtcacccca catggcgcca gagctaccct
     3421 cccacgggac cgcgagcggc tctttgccct gcggcagcac accccgacca ccggtctgct
     3481 gacccttctg taaccctaga acttgtttct ccaggccctg accctggagc ggcgccagct
     3541 ctgctagacc cgcatctagg gacggggtgc tttccggctg aaaacctccc tgccagcata
     3601 gcggcaatgc ctcgctggta cggtcggcga ctagacagtg acctggcggg cccgcgccgc
     3661 ccaggtgacc atcctggtga aggctcacct ggttgataac cttaccggaa ccgcgtctct
     3721 ctacacccag cggacccggc gtaccttgtg gagccgctaa ttccggttgc agatcccaag
     3781 agcttggact tggcagagtg ttgccatcac gatgcatgcc acgcgggctg tctaatgctt
     3841 gttcctgacc gccgtgaacc tcgcgagcac gtttgccgga ggagctaact ttacccagac
     3901 acgcagcgcc acccgcgcct tgcaaccccg gggacggacg cagacggccg tcaccgtctt
     3961 cgtcctcaac actgccggac ggggagctat ccagttgtgc gccgctgaag gacggcgggg
     4021 cctcggcgtc ctcttcttcc tccaacgcca tcaatctttt ctgcaccagt tgagctaggg
     4081 tcaaaccctc ctcttgttca agttcttcga tgagtgcgag aggatcgcgc tgcttttctg
     4141 gactcagtag atctgccagg aactgcggat ggatcactgc ttccaccttg gaaacaaaga
     4201 ccttctggct gcacagttcg ttaatgtaac ttagtaaccc cgggtccgga tacatacctt
     4261 cttcctcttg ttgctggcct tcctcctcct gcttgccgtc gctttcacct gtcgccaggt
     4321 gcgtaccgac aagccattcc atgatgtcaa cgtattcctt caccgcctcc ggcggaatct
     4381 ccttcggcgc ctccggagcc ggcggacgct gcgctttacg ctggcggcga cgtggcgcac
     4441 gggttttgct tgccgctttt ttcgggatgt aaaccggttg ttggcacacc tcgcttgcca
     4501 gcggtcccaa agggtccagc ttaagcgggg tcgctgggct cagaccttgg gagccattca
     4561 tgagctgggt gttttggatt tgcatctcct ccgcttcaaa ttccataaaa cgttctgcca
     4621 tttcgtaaaa gatcatgcga tcgaagttgc tggtgtgttc ccattcttga acagcgagcg
     4681 gcaggccttc ctccagggtc atggttggtt tcagacgtgc taaagagcgc agcaccgggc
     4741 tatagttgtg aaaccagtta attacagtag aagtcttcag attcagctgg ctcagaccca
     4801 gcaccttttc cccgaagatg cgctggcaga tgccgttttt tgccagcttc tctttaacct
     4861 gacgcgtcaa ttcgatcgta tcaacctcct gatacatgta aacttcatat tgttccgggg
     4921 tcagcggcgg cacgctcggc tttgtcggct tgctcatcgg cacgatggga gagctcggga
     4981 gcgggctatt ctgcggggtt tcgctacggc tcgcgccggt gagagacagt tcgctcgact
     5041 ggctgtaggg gctttcagct gaaggccact ctttccagta ctcactgcta gaaggacctt
     5101 gcaactggga tctctccgca cggggctggc ttccgccacc gctaccgcct gagcccttct
     5161 ccttaccacc acccgtctct tctgccttcg cctcctcgct agacgccgcg ttacgacgtt
     5221 ctggttgaac cgcgctccac caatgatctt tccacgcacc gctacgaccc acttcattct
     5281 tcacctgacg caacacaccc tgagcacaat ccgcagcgcc ctgcgggtcc agtccaggag
     5341 cgtcttgggc ttccttcttt aatgccggcg gattcggcag cgcggacgca cccgcctccg
     5401 gggcagcgct cggttttttg agggaaatcg ccaaaggcgg gtagctgcta acagtcggca
     5461 tcggggaggt gctcaacaat ttcggcgtca agatggtaat gtcggattgc gacagcggtg
     5521 cctgctttag agccgggtcc agcgccgctt gctgggcttc catttcacga cgggcttgtt
     5581 gcagaatact acgaatagcg tcatcactgt taccggagcc agatgcactc gacggttgtg
     5641 ccggctctgc cgtcttctga acttgaagct cacgctttgc ctgctccagg atgcttttaa
     5701 tggcctcgtc gctaccggtt tcggaagcgc ggattctggt ggtaatgtta ccttcagaac
     5761 cgttacggcg cttcggtacc tcctgaaaca ggcggttcaa actctggccc ggattctcac
     5821 gctgacgtcc ctggatgctt ctcagcgcca gaatgttttg ctcgtcgctt aagaactgtt
     5881 tcattttgtg gaacggctcc ttaccacgaa ctgtcagctt attccacggt ttcggccgtg
     5941 ccaggatctc gcttacgctg ccttgggaca gacccagtac ataatgacca aaaatacgct
     6001 gaccaatatt gtgtttgatc agctgttcct taacttgacg tgctatttcg gcggtgtcca
     6061 tctcttcgcc ctcagacaca cttcctgcgc tctcgctttg gctaggagac ggcgccatac
     6121 cattaacatc gggggactgt tggagcgggc tctggctgga aatgctgttg gtggagtacg
     6181 gaccggtgct gaaaatcatt gaggtactac cagcctcctg catggcctta gaatagaagc
     6241 tctgcatcag ctgtcgttgg agcaggctgt tcagagcaaa tttaccggta gatgccagcg
     6301 gcaagctcgg cgacgccaga gagctgctaa agaaatcctg gctcaggcct gccgggctaa
     6361 attggtgggt accattggta ttgctcgcct gctcccccgg attacgtggc agctgggacg
     6421 gcggtggcgc cggcaggcta cccggacgac ggctctctgg ctggtccttg cccttgcgac
     6481 gtgcgctgcc ggacaggtct gagttggaga tgcgcagagc tgcgttttcg gactgcagtg
     6541 aacggttttt ctccagcagc agaacctcaa ggggtttggc ggcgtcctgt gtccctgcac
     6601 cctcgcttgg cgcgaactcc atcgacttca ggatattcag ctcttttttc acctcttcat
     6661 aatccgcttg gcccttcagc ttttcctcta actgctttag ggtgctgttc ttcgccgaga
     6721 gctgttgttc caattgagag atttgactcg cagagttctc gcgcagcttg gtcaagctcg
     6781 cctgcagacg ctgcacatct tcgaccagtt gtgcgatttc gcgttcttta gcggcaagtt
     6841 caacttcaag gctgctacgg gtgagaacct cgatcgcctg ttcaacgtcc ggagccttct
     6901 gtatttgaga agctaattgt aaactatgat tcgcgctaga cagttgttca cgcaatgtct
     6961 ccgcttcgcg ctgggccact tcggctcgct gatttgcgcg ttccaaatcg gtcataatca
     7021 tctcgatctc atcggccttt gcggtggttt cttcatcata tttggtcttc aaatcgaaca
     7081 attcggtgcg ggttttctcc aaagccgttt gaagcgattg aactttgtgt tcagcttcct
     7141 ccagcttgga tgtcgtagac atctgggttt cttgcagttt gcgctccttc tccgcgaaat
     7201 cgttttgcag cttctgctcc ttctccagag cgattgtttc ggcttggttc ttcagcgtct
     7261 gttcatattc acggatcttc tctttcagcg ctttgatggt aacttcttgg tttttaacct
     7321 cggcgaactc tttattgtat tcctccaggg tttcacgtaa tttctggttc tccgtttcaa
     7381 tatcgtgcag acgctgcact ttcagttgca attgttggcc caggtccagc gccggaaccg
     7441 ggtccgggac atcgatcaga cgtttgtaaa cattcagaaa cgcggcttca gcttccttag
     7501 aacgtttact taaggcatcg atttcgccct ggaaactctt cagcagcggt gcgacctgtt
     7561 tgcgcaggtc ctcaggggtg ttttttttga actcacgact ttgttcgatc agacgcttac
     7621 gggattgctc gctttcgtcc tgacgattgg ccaataccgt ggcggtcgcg tccagctccc
     7681 gctgcagttg ttgcagatca aagcgtttcc agtattgaaa cattgatcct acattagctg
     7741 ccattccccg ggaagcggcc gcacttcccc cggagcctcc gcttccgccc ttgtacagct
     7801 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
     7861 gcttctcgtt ggggtctttg ctcagcttgg actgggtgct caggtagtgg ttgtcgggca
     7921 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
     7981 tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt
     8041 cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt
     8101 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
     8161 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
     8221 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg
     8281 ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca
     8341 cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc
     8401 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
     8461 ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggtg gcgaccggtg
     8521 gatcccgggc ccgcggtacc gtcgactgca gaattcggtt cactaaacga gcccggttca
     8581 ctaaacgagc tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca
     8641 atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac
     8701 tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac
     8761 gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga
     8821 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
     8881 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
     8941 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     9001 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     9061 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
     9121 ctaatgaccc cgtaattgat tactattaat aactagcacg cgttcgaagc ttgagctcgg
     9181 tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct
     9241 attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat
     9301 tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc
     9361 ccatttatct aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc
     9421 aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt
     9481 cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc
     9541 agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag
     9601 gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg
     9661 caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc
     9721 agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc
     9781 tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg
     9841 caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt
     9901 atatttatat aattcacttc tccaattgtc cctcatatct cctccttatg gtgttttact
     9961 aaacttttcc atgttctaat cctcatcctg tctacttgcc acacaatcat cacctgccat
    10021 ctgttttcca taatccctaa tgatctttgc ttttcttctt ggcactactt ttatgtcact
    10081 attatcttgt attactactg ccccttcacc tttccagagg agctttgctg gtcctttcca
    10141 aagtggattt ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg taatttgttt
    10201 ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact
    10261 gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca tttgtactgc
    10321 tgtcttaaga tgttcagcct gatctcttac ctgtcctata attttcttta attctttatt
    10381 catagattct actactcctt gactttgggg attgtaggga atttccaggt ctgaagatcc
    10441 tgctgcttgc tgtgcttttt tcttactttt gttttgctct tcctctatct tgtctaaagc
    10501 ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt
    10561 atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat
    10621 ttgtctacag ccttctgatg tttctaacag gccagtatta actgcgaatc gttctagctc
    10681 cctgcttgcc catactatat gttttaattt atattttttc tttccccctg gccttaaccg
    10741 aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc
    10801 tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc
    10861 gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt
    10921 ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa
    10981 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga
    11041 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc
    11101 tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaaag cagctgctta
    11161 tatgcaggat ctgagggctc gccactcccc agtcccgccc aggccacgcc tcccatggta
    11221 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
    11281 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
    11341 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga
    11401 cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat
    11461 gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg
    11521 aactccatat atgggctatg aactaatgac cccgtaattg attactatta ataactagtc
    11581 aataatcaat gtcgacccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
    11641 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
    11701 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
    11761 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
    11821 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
    11881 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
    11941 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
    12001 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
    12061 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
    12121 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
    12181 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    12241 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
    12301 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    12361 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    12421 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    12481 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    12541 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    12601 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    12661 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    12721 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
