LOCUS       sequence-488266-       11373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein NUP98_NSD1 with an IDR deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11373)
  AUTHORS   Maiti S, Tripathi S, Baggett DW, Phillips AH, Park CG, Wang J, 
            Wahiduzzaman, Freyaldenhoven WT, Kinger S, Pioso BJ, Bollinger JC, 
            Somjee R, Lang B, Babu MM, Kriwacki RW
  TITLE     Proteome-wide computational analyses reveal links between protein 
            condensate formation and RNA biology.
  JOURNAL   Sci Adv. 2025 Dec 5;11(49):eady1420. doi: 10.1126/sciadv.ady1420. 
            Epub 2025 Dec 3.
  PUBMED    41337585
REFERENCE   2  (bases 1 to 11373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-12-5"; volume: "11(49):eady1420. doi"; pages: " 
            10.1126/sciadv.ady1420. Epub 2025 Dec 3"
COMMENT     Sequence Label: CL20_mEGFP_NUP98_NSD1-Delta-1-470
FEATURES             Location/Qualifiers
     source          1..11373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4906..5616,5617..5619,5620..5622))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4948..4969)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5297..5316
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5556..5577
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5619..5628
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5704..5907)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5733..5753)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5908..6211
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6468..6512)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6697..6930
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7290..7407
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7899..8024
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8071..8328
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        8330..8709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8740..8844
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8845..8913,8914..9705)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9063..9082)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9876..10464
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10365..10384
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10618..10635
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    10703..10837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10727..10746)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10781..10800
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(10843..11038)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10857..10992
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    11097..11118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11133..11138,11139..11156,11157..11163)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11171..11187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11176..11198
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11195..11211
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11195..11211
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca ctacttctgt tctgattctg cacacttgtg
      601 actggaagga gcctggttaa gagctggaag tgtattttgc tctggtttgg ggtcttgccc
      661 tcttccaaga gaccagcaag tctgtgccaa tgtctgtgtg ctcccagcag accagcaaga
      721 ctgtgccagt gtctgtccag ccactatggg ccagagccct gcccgtgatg aggcttttcc
      781 cagggcccag ggactttgct ctgtggttac caacttcttt tcttcagtgg ggagggaggc
      841 tggggccttc ccgagagacc taaaagattg cccactcttg gcggcaagtg caggtagttg
      901 ctggcctccg accatctgct tcgcctgctt caccaggccc ggggattggc taagaggccc
      961 tggggtctgc tcagcccctg cagaagctct tcctgaggcc tgagttgttg gctcatataa
     1021 aaaggccttg gcaggaggct gagaaagaag tctggcctgg gtgagtgatt taacagcttg
     1081 ccaggggtcc tctgaagggg ctgctgcttt cccagatgtc tgaagaggat ctgacacaca
     1141 gcctttctca acagctctcg gcatatgccc cagacctttg ctggcaggcc atgaacttga
     1201 ctccaacact ggcatcttct catcagcctg tggtgtgacc tgatgaggtg aagctgcccg
     1261 ctccttctgg cgaggagtta ggtctatgag atccatcacc aaagcagctc tattttttga
     1321 ctgagtattc tggtccacag gcctcagtgc tttttcttta gctacaaggg tctggaccac
     1381 agctgatagt actttctcag gaggtgggag tctctgggac aaagaggcat ggggtttgtc
     1441 agtaggcctg tctgaagtct ggggtttggg actgctagta atgagcagtc tgtctggcgg
     1501 cggaagtctc aggccagtgg gaactgaggt tttctccagt gactggggcc ttgggctggc
     1561 agcacctatg gatttatcca gccttgggtc agccgtcccc agaggtcttt ccagtggttg
     1621 ggacctgact gagggtgagg agcttgggct ggtcactgga gagggtttgt cgcttagctg
     1681 gggtcttggt cctgccactg ctggtggcct gtccagtggc ctctggctgg aaaccaagga
     1741 ttgggatttg gtccctgacc cagcgaggtc tctgacctta tctaaaggct ggggcctgga
     1801 gtcagttctc tcaagaggtc tttcaggtag caatggcctc tgacaagtcc ctgccagagc
     1861 ttttttggag agcgacagca tctggttggt gtcagcagga ggtttatctg acattttggg
     1921 tgcctgagca gccattcctg ttgattgctc tgccaggtga gtgcttggcc ctggaggcag
     1981 cggtactggg ggaggcacat actcacggat ctccccaggt tccagaggat tgggcccaca
     2041 ggggtcatgc tcagtacaag acagacgccc atccagtttg gaaatgaaaa gcatcccttc
     2101 tcgatgctgc ttacaaaagg agctggggca catctcacag aaggaggctg cttccttccc
     2161 gcagatgtca cactgatgcc acggacattc ccatttccct gctggtcgct tggtcagatt
     2221 gagacagtct gcgtggtaaa cttttgggca gcctggtttc ttgcaggaga cgagctggcc
     2281 agcatcccca caactaaaac actcatcttc tcgctccttt gtgatttcac cctgggtcct
     2341 gcgctttccc tgttgcttct tcttgaattt ctttgacttt tcttccgtgg caatgggttg
     2401 attctttggc cttacaccca agaagccact gcagttcggg gctccacatt tgcaaacagt
     2461 ctttccattc ccaagacatt ctaggttgta gttgaaggta agttcagtgc ctgctttaat
     2521 gtcacttagt gcaaaaaggc ctacacgggt atctccattc acagaccact tctgtgtttc
     2581 acagttgggc tggcagcaat gattcatgaa ccgagcatag tttcctttgg gaccagcatc
     2641 aatgattcgg tctttgtcta gggtgagcat atagaaatta gtgatatcat gttcttgagc
     2701 atagcgaatt cgagctctgc attcttcttc atctataagc tcacccacat actcattcac
     2761 aaattcaccc tttttaatat ctgtttttgt ccgtagaccc caaccccgct gtaatgtgcg
     2821 gaaaatttca acctctggat attggcgctt ggaaaagcac tggttttgac agcgccctcc
     2881 ggcaggacac actgtggggt ggcactcata gagcagcatg cggttgatgc attcagagtc
     2941 tatcccacag gggttctcat cagtagcttt acagttgcaa cggggtattt cagataagtc
     3001 tgcagtgaag atctgtaccc tgccaatagg acggtttacc tttatatgtt tataaggtgg
     3061 tggcttcttg tcattctttc ggtcttcctg cagctgtctt agctcttttt gggcctttaa
     3121 ttcctcaaac cttgctgcag cttcctgaag agctttttta tatgtcccat ccactccttt
     3181 gcccatctta tccttgctgc tcacgtcacc ctccatgtaa gggaagactc gggcctggtg
     3241 agtccacaaa tagtcattag atccaaaaaa gaggactggg aactctccca catcatgtct
     3301 catcttatca atgttggaag gaacagctcg aggatggcag atctcagctg gccaccacct
     3361 gtatcgtcca acttttaccc agacaatctc cctgtagtgt ggctttttgc ctgctttaca
     3421 gtcattgcaa taccagtttc cttcagggat atcaatgttc aggcattcac gatgaaaagc
     3481 agcagggcaa gaatcacagc acagaaggct gcctccttct gagcacacaa agcaccagct
     3541 aacattaaca tgctcatgat ttcggcagcc ccgcctaggg gtaaagtgat tagggcagat
     3601 gatactatta gatgcaagga tctttgaccc agcagccagg caaaagtcat tggcgtggta
     3661 tgccacagga cagcggacac agcgcatcaa ccgaccttta gatgcagaaa cattggctgg
     3721 attagcagca tgacaggtta tacagatgtg gagggagcac cggaagccct tgttctgcat
     3781 aacagtgggt gggtacttct ggacacactc ttcatggtaa aactttccac acaagggtag
     3841 aaggcacctt ttaacatctt ccccactctg cttacataca aaacaggtat ggattcctgt
     3901 gcgacattca ttgcagataa attttcctct tggcatctca gtcaatccaa ggcactccag
     3961 gtggaaagcc ccacagcact gagcctcaca taacagcagc tcacccaatt tttcacaatt
     4021 ctgacagaca ttctccttga gtgcagctcc tccaccgcgt tcaccctgca tttttttaga
     4081 ggcaggcatc ccgggatcgt gttctacacc ttcctcaaca gtctccttgg ggcttgtggc
     4141 cgtcctgtga ggcattagtt ctccctctga gcctggaatc tcttttgacg agtcatcatt
     4201 tttcactttt ttacactgca tcttggctgc agcatgcctc tgtcgttttc gcttccttgg
     4261 cttgtcaaat atcttggtag tgcctccacc aaaatctttt gaataagatg tggcacaaga
     4321 ttcagttatg ccattctcca ggtgaccagc ttcatagcac tttttagaaa ggccaaggtc
     4381 ccccttcttg ggcataaatc cagggtctaa atcattggat tcaagaagtt tcttagttgg
     4441 ttttctttga cgtttacttt cataatttcc tggcgtttta actagcaatt cctcagactc
     4501 aagggcaggc cgtggagaga cttccggagc cacaggcaca gacaaggtca gctgcggcaa
     4561 ctcagcatgt ccacctccaa gttctgactg agccaagggg ccctccaaaa acggagcctc
     4621 cctttcaaga actggaggct cttctttggt tgaaatcaaa gaattctcgt ccacctgctt
     4681 gttctgagca ctagatcgac ctcgggctag aaggctttcc tcttcacagc gggaacttac
     4741 cttgtgcacc tgctcctgta ccttcttttg ttttttctta ggagcaaata tctgatcata
     4801 ttcttctgta tattccaaaa gccacttgct tggcttccta aggcgtttct tctctgaccg
     4861 cacccgggaa gcggccgcac ttcccccgga gcctccgctt ccgcccttgt acagctcgtc
     4921 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
     4981 ctcgttgggg tctttgctca gcttggactg ggtgctcagg tagtggttgt cgggcagcag
     5041 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
     5101 gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc
     5161 catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc
     5221 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
     5281 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
     5341 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
     5401 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
     5461 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
     5521 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
     5581 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggtggcga ccggtggatc
     5641 ccgggcccgc ggtaccgtcg actgcagaat tcggttcact aaacgagccc ggttcactaa
     5701 acgagctctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg
     5761 ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc
     5821 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc
     5881 attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta
     5941 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
     6001 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
     6061 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
     6121 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     6181 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
     6241 tgaccccgta attgattact attaataact agcacgcgtt cgaagcttga gctcggtccc
     6301 ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc
     6361 actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg
     6421 aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat
     6481 ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt
     6541 aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg
     6601 tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg
     6661 gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat
     6721 ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac
     6781 agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac
     6841 aataattgtc tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct
     6901 gctcccaaga acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc
     6961 actcttctct ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat
     7021 ttatataatt cacttctcca attgtccctc atatctcctc cttatggtgt tttactaaac
     7081 ttttccatgt tctaatcctc atcctgtcta cttgccacac aatcatcacc tgccatctgt
     7141 tttccataat ccctaatgat ctttgctttt cttcttggca ctacttttat gtcactatta
     7201 tcttgtatta ctactgcccc ttcacctttc cagaggagct ttgctggtcc tttccaaagt
     7261 ggatttctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt
     7321 aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac
     7381 cccccaatcc ccccttttct tttaaaattg tggatgaata ctgccatttg tactgctgtc
     7441 ttaagatgtt cagcctgatc tcttacctgt cctataattt tctttaattc tttattcata
     7501 gattctacta ctccttgact ttggggattg tagggaattt ccaggtctga agatcctgct
     7561 gcttgctgtg cttttttctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc
     7621 ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat
     7681 aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt
     7741 ctacagcctt ctgatgtttc taacaggcca gtattaactg cgaatcgttc tagctccctg
     7801 cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt
     7861 ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc
     7921 tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct
     7981 cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct
     8041 ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg
     8101 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
     8161 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
     8221 aggctcagat ctggtctaac cagagagacc cagtacaggc aaaaagcagc tgcttatatg
     8281 caggatctga gggctcgcca ctccccagtc ccgcccaggc cacgcctccc atggtaatag
     8341 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     8401 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     8461 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     8521 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     8581 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     8641 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata
     8701 atcaatgtcg acccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     8761 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     8821 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     8881 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     8941 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     9001 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     9061 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     9121 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     9181 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     9241 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     9301 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     9361 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     9421 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     9481 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     9541 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     9601 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     9661 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     9721 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     9781 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     9841 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     9901 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     9961 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    10021 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    10081 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    10141 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    10201 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    10261 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    10321 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    10381 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    10441 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    10501 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    10561 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    10621 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    10681 tggccgattc attaatgcag cggatccaga catgataaga tacattgatg agtttggaca
    10741 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
    10801 tttatttgta accattataa gctgcaataa acaagttagc tttttgcaaa agcctaggcc
    10861 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
    10921 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
    10981 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcgg
    11041 atccgctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
    11101 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
    11161 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
    11221 gccaagcttg ggctgcaggt cgagggatct ccataagaga agagggacag ctatgactgg
    11281 gagtagtcag gagaggagga aaaatctggc tagtaaaaca tgtaaggaaa attttaggga
    11341 tgttaaagaa aaaaataaca caaaacaaaa tat
//
