LOCUS       sequence-488290-        6533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Kaps I, Ehrt S, Seeber S, Schnappinger D, Martin C, Riley LW, 
            Niederweis M
  TITLE     Energy transfer between fluorescent proteins using a co-expression 
            system in Mycobacterium smegmatis.
  JOURNAL   Gene. 2001 Oct 31;278(1-2):115-24.
  PUBMED    11707328
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2001-10-31"; volume: "278(1-2)"; pages: "115-24"
COMMENT     Sequence Label: pMycVec2
FEATURES             Location/Qualifiers
     source          1..6533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     570..586
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     571..587
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(718..745)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(835..852)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1006..1594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1086..1105)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     regulatory      4989..4998
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttggcctgct ccttggtcaa gcaggtggct acgggtgcgc cgtgaatgtc gacgacattc
       61 caggagaagg aatccggggt ggattggata tgccaggccg ccggcggatt agcgaagtgt
      121 gcgcgagcga gctcttcaaa gacgacaagg ctcattggtg actgcccacc tttttgggtt
      181 caaacgttac ggaaggtttc taacgaaacc gggtcgtcta gactctcttg acagtagggt
      241 cgccgagagc ggctgtcaag acagtcttga ctttgttatc gcttaagcgt gaggtggcaa
      301 ccgtggatga aagcgacgcg ctcgcagagc tggtgcgcgc gcacgcagac ttggaacgcc
      361 tcactgagga catcgcggac gcacgagagc ggcgccggca ggcggcgagg cggctggtcg
      421 acagtggaca cgggacgacg tggatcgcaa accaactcgg tgtgacgaag caggccgtgg
      481 acggtttcct gaagtacaag gagcgcaggg ctctgttgca aaaatcgtga agcttgcatg
      541 cctgcaggtc gactctagag gatcccccct cgaggtcgac ggtatcgata aaataaaaaa
      601 ggggacctct agggtcccca attaattagc taaagcttga tatcgaattc ctgcagcccg
      661 ggggatccat ttaaatcagc tgttaattaa tctagagttt aaacagtatt aaacgcaaaa
      721 aggccatccg tcaggatggc cttctgctta gctaatcaac tagttctagc tgcattaatg
      781 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
      841 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1081 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1141 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1201 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1441 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1681 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1741 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     1801 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gacgtggccg accagcccgt
     1861 catcgtcaac gcctgatccg cggtgcggac aggccgtgtc gcgaccggcc gtgcggaatt
     1921 aagccggccc gtaccctgtg aatagaggtc cgctgtgaca caagaatccc tgttacttct
     1981 cgaccgtatt gattcggatg attcctacgc gagcctgcgg aacgaccagg aattctggga
     2041 gccgctggcc cgccgagccc tggaggagct cgggctgccg gtgccgccgg tgctgcgggt
     2101 gcccggcgag agcaccaacc ccgtactggt cggcgagccc ggcccggtga tcaagctgtt
     2161 cggcgagcac tggtgcggtc cggagagcct cgcgtcggag tcggaggcgt acgcggtcct
     2221 ggcggacgcc ccggtgccgg tgccccgcct cctcggccgc ggcgagctgc ggcccggcac
     2281 cggagcctgg ccgtggccct acctggtgat gagccggatg accggcacca cctggcggtc
     2341 cgcgatggac ggcacgaccg accggaacgc gctgctcgcc ctggcccgcg aactcggccg
     2401 ggtgctcggc cggctgcaca gggtgccgct gaccgggaac accgtgctca ccccccattc
     2461 cgaggtcttc ccggaactgc tgcgggaacg ccgcgcggcg accgtcgagg accaccgcgg
     2521 gtggggctac ctctcgcccc ggctgctgga ccgcctggag gactggctgc cggacgtgga
     2581 cacgctgctg gccggccgcg aaccccggtt cgtccacggc gacctgcacg ggaccaacat
     2641 cttcgtggac ctggccgcga ccgaggtcac cgggatcgtc gacttcaccg acgtctatgc
     2701 gggagactcc cgctacagcc tggtgcaact gcatctcaac gccttccggg gcgaccgcga
     2761 gatcctggcc gcgctgctcg acggggcgca gtggaagcgg accgaggact tcgcccgcga
     2821 actgctcgcc ttcaccttcc tgcacgactt cgaggtgttc gaggagaccc cgctggatct
     2881 ctccggcttc accgatccgg aggaactggc gcagttcctc tgggggccgc cggacaccgc
     2941 ccccggcgcc tgacgccccg ggccgcccgg cgccgccccc ggcccccggc ggccgccggg
     3001 cggagccccg cccggcggcc cgggagcccc ggatcccctg cccggctggt ctcatgttgg
     3061 taaaccaccg acaccaaacc gggcaacttt tggaggtctt tttcgcccgt ggtcggattg
     3121 tgaacgcggg tgtatccgac gtgatcggac aggtaagcca tcgcctcttc gacggcacgg
     3181 ttggccgcgt cctcgacggc tttttgcgtc acatcgttgc cgaatgcgcg cagcagactg
     3241 acacttttcg gaacgctgaa agtgacgtcg aaaccgtgat tgtcggtggc gcgaaaggct
     3301 cgaccgtgcg ccccattcgg agcgatgccg tcgttgaacc agcgcgttac tgccgcgctg
     3361 tccgcgtccc cgccggcgca ctgctccggt gataaaccgg tcagttctgc gacccgttgg
     3421 cgatccccag acaccagcca aaccggctgg cgtgtgtcgc gctcggagta gtactcaccg
     3481 agcccgccgt tgccgtatcg ggtgtcgcgc gcggcccggc cggcagcggc agcagtgtcc
     3541 tcgtagtact tgatccccca cgccttgagc ggggtcacat tagccaccac agccggtcac
     3601 gctcccaacc ccaaggcccc ggggcggggg ccttccggta gcacacggac gtgtgctacc
     3661 gccagtcttg cacaccttgt gccaaagtgt gaactctaaa tgcttgttat caaggaagta
     3721 gctggggaac tggacactgg attggaagct gggaggcagg cgagggaagg aggcgaggac
     3781 aaaacaggac aaaacaacaa agcatctgat ctgggaaaag atggcaggcg agggaaaaca
     3841 tgctgatcaa accgaacgat tgtggaaagt gaaatcgctg gccgctgggc ggcttcggcg
     3901 ggccgacgat ggaggtcgag aaccaagcat catcgcaacc gctaattgag ccgagtggaa
     3961 gcgacgagcc ggttgctatt tttcgacggg atacaggagc aagtgcagtc gaaccttctg
     4021 agttggctgg gcatcgagat agctccgctc tcgctaacga acagcgctgc gggctcatgt
     4081 agatcagcgg gggctacggg cagtctgaac cattgcgtcg aattgagcgg tttcggccgc
     4141 gacgacgacg gtgcgtttac aggcggcacg acgcacagcc tctgcaacgg cctgacgcgc
     4201 tgcggcgtga ccaggaccgt ctaaggcagc ttgggcagtg gcgcgatcgg tcgcatattc
     4261 gtcatgtgct cggaactggt cgcgcacgca acgtcgggca gccgcaagtt cttcagcctc
     4321 acgcgcttcg tcgagtgcgg cgggccgatc ctccagactg ttgtggctgg tgtgccaggc
     4381 cagcatcgtg cccaacaaag cgatgggccg cgccggcaca tcgggcaccc agtgaccggt
     4441 gctcaaccag tcgcgtacca acgccgtcag gtcggccggc gtccaaccgg cggccgcggg
     4501 tgcggccaac atcgccgccc agctgtcggc ggcatacgtg cgaacccagg gcggagcgtg
     4561 ccggtcggcc cgccacctgg tggccagccg gcggccggcc tcgtcaggtg ctcggcggcg
     4621 cgcagcgccg tggtgccggg cgcttgcgcc cgcctgggta gtgaccagtc tcttcaaagg
     4681 tgagttttta gtggttgctt gagaccgttc caggtggggt gacaagctgt ggacaactcg
     4741 ggcgatgtgg gggttgccgt gcagtgccca caccgacgcc cagccgcggt ggcgatcgcc
     4801 catccgccac gacgccatgc gttcggtgta ggtgcgttgg cggccacgca ggacctcggt
     4861 ggccaccccc agcaagcgca ggcattcgtg cgcccgctgg atggtgcgct catcgaaacc
     4921 ggtggccgct tcgagctgct cgttggtcgg ccgacagttg cggccggtat cccagtcggc
     4981 ggtctgcgcc atggcggcgg cgatgacgat caacgtggtg cgggcgatgc cgccgttgca
     5041 catgcgcggc cgcaccatgg cgtagtgcac gtcgtaagcc actgcgacgg tgacgtgcgc
     5101 ccagtgcgcg gggccaccgc tccagcacgg cacgccggca taggcgtcgg cgtcgagctc
     5161 caggccgacg aactcgctcg gtgccggcat ggcgcgccct gccagtcgcc ggccgcgacg
     5221 gatcgccaac acctcagcgg tgcgggtggt gcgcaggaca ccgcgccggc gcacggtcgg
     5281 gctacagccg cggtacgtcg gcggtgtggg ggtgaacggt cgtcggcaac ggtcgccggc
     5341 gccgttttgg tcacggtttg cacgcaagat gcgcggcgta tctggtacgt gcgcaaggtg
     5401 tgcgctaccg tgagacgagt ccagcaagac acgtcccttt caggggcaca ggtgcggacc
     5461 tgaaccgggc ggcaacccgg ttcgaaccct tgtgacaggg cctcgctacg gcgaggcctt
     5521 cgtcatgtca ggcggtctgc acagctattc agatgtcacg tgcgtccggc ggtcgaccac
     5581 gacaagccaa aagaaaagtc gccggactgt cggtgtcaca tcgccagcag cagaacctcc
     5641 ttgtcgagtt cgcggacaag gtgcgggaag cccatgtggc ggcagatgat gtcaccgagg
     5701 taggtggagc gctgcattcc gagtcgctga tggtcgcgct cgacgagctg agcgatcggt
     5761 agcggcagac ggactttcgc gtgcggtccg atcgtgtcgt cggtatcggc ggtggcgggc
     5821 tgcgattcaa acaacagctg ctgcggcagg tgacgcatca gatcgggccg gccgtagtgg
     5881 aaacagacga tgtcggtcag cacggcggac cggtcgatgc cgtagtcagc cgcggtcgcg
     5941 attgctgcat tgagcacggt gacggggagt gttgcggtca tgacggctcg ctcgcccagg
     6001 gacgggcggc ctgctcgagt tgatccggtc atacgcgcga tcctgccggt agctttgccg
     6061 gaaatcacga agccacgccg gtgtgcgcgg cagatttccg gcaatcgccc caaatcgccc
     6121 taccgtcagg ctgagatgct cgtgttcagg ctgaaccgca cgtggccgtg tctgaaagaa
     6181 gatcgcgcag gaatgcagcg agatcggtgg tcgccgcgat cgatctgtgg tcaagaaaat
     6241 caagatcctt ggcacggtag gtgtggaagt agtcgccatg gagcaggtat tggccgtcgg
     6301 cgagaagcgc tgcgtcccaa atccgatcgt cgtcggcact ctggtcgaga aagcgcaccg
     6361 ggcggagtgc gcttgcagaa cggaactgtt cggcggctct actgaaggat tcgacgatgt
     6421 cggcgatgat gagccgttcc gcacaagtga gagctctgcc tcgaccgtaa cccaggtacc
     6481 agtcggttcg ttggtaccac tgagcggcgg cgaacccgga gtggcgtgac tcg
//
