LOCUS       sequence-488316-        7962 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Distribution and localization of human wild-type Munc13-4 in cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7962)
  TITLE     Martin lab plasmids
REFERENCE   2  (bases 1 to 7962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DsRed1 Munc13-4
FEATURES             Location/Qualifiers
     source          1..7962
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1230)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(736..756)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     954..973
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1148..1171
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    4690..4811
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4727..4746)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4781..4800
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4818..5273)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4955..4976)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5167..5186
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5300..5404
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5406..5763
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5406..5597
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5429..5449)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5614..5749
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5798..6592
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5798..6592
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5852..5871)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6462..6481
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6780..6799)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6824..6871
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7200..7788
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7689..7708
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgcg ctcctccaag aacgtcatca aggagttcat gcgcttcaag
      601 gtgcgcatgg agggcaccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      661 cgcccctacg agggccacaa caccgtgaag ctgaaggtga ccaagggcgg ccccctgccc
      721 ttcgcctggg acatcctgtc cccccagttc cagtacggct ccaaggtgta cgtgaagcac
      781 cccgccgaca tccccgacta caagaagctg tccttccccg agggcttcaa gtgggagcgc
      841 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      901 ggctgcttca tctacaaggt gaagttcatc ggcgtgaact tcccctccga cggccccgta
      961 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
     1021 gtgctgaagg gcgagatcca caaggccctg aagctgaagg acggcggcca ctacctggtg
     1081 gagttcaagt ccatctacat ggccaagaag cccgtgcagc tgcccggcta ctactacgtg
     1141 gactccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga gcagtacgag
     1201 cgcaccgagg gccgccacca cctgttcctg ctcagatctc gagctcaagc ttcgaattct
     1261 gcagtcgacg gtaccgcaat ggcgacactc ctctcccatc cgcagcagcg ccctcccttc
     1321 ttgcgccagg ccatcaagat aaggcgccgc agagtcagag atctacagga tcccccgccc
     1381 caaatggccc cggagatcca gcctccatcc caccacttct cccccgagca gcgggccctg
     1441 ctctacgagg acgcactcta cactgtcttg caccgcctgg gtcatcctga gcccaaccat
     1501 gtgacggagg cctctgagct gctgcgatac ctgcaggagg ccttccacgt ggagcccgag
     1561 gagcaccagc agacactgca gcgggtcagg gagcttgaga agccaatatt ttgtctgaag
     1621 gcaacagtga aacaggccaa gggcattctg ggcaaagatg tcagtgggtt cagcgacccc
     1681 tactgcctgc tgggcattga gcagggggta ggtgtgccag ggggcagccc cgggtcccgg
     1741 catcggcaga aggctgtggt gaggcacacc atccccgagg aggagaccca ccgcacgcag
     1801 gtcatcaccc agacactcaa ccccgtctgg gacgagacct tcatcctgga gtttgaggac
     1861 atcaccaatg cgagctttca tctggacatg tgggacctgg acactgtgga gtctgtccga
     1921 cagaagcttg gggagctcac ggatctgcat gggcttcgca ggatctttaa agaggcccgg
     1981 aaggacaaag gccaggacga ctttctgggg aacgtggttc tgaggctgca ggacctgcgc
     2041 tgccgagagg accagtggta ccccctggaa ccccgcactg agacctaccc agaccgaggc
     2101 cagtgccacc tccagttcca actcatccat aagcggagag ccacttcggc cagccgctcg
     2161 cagcccagct acaccgtgca cctccacctc ctgcagcagc ttgtgtccca cgaggtcacc
     2221 cagcacgagg cgggaagcac ctcctgggac gggtcgctga gtccccaggc tgccaccgtc
     2281 ctctttctgc acgccacaca gaaggaccta tccgacttcc accagtccat ggcgcagtgg
     2341 ctggcctaca gccgcctcta ccagagcctg gagttcccca gcagctgcct cctgcacccc
     2401 atcaccagca tcgagtacca gtggatccag ggtcggctca aggcagaaca gcaggaggag
     2461 ctggccgcct cattcagctc cctgctgacc tacggcctct ccctcatccg gaggttccgc
     2521 tctgtcttcc ccctctctgt ctcggactcc ccagcccggc tgcagtctct tctcagggtc
     2581 ctggtacaga tgtgcaagat gaaggccttt ggagaactgt gccccaacac cgccccattg
     2641 ccccagctgg tgactgaggc cctgcagact ggcaccactg aatggttcca cctgaagcag
     2701 cagcaccatc aacccatggt gcagggcatc ccggaggcag gcaaggcctt gctgggcctg
     2761 gtacaggatg tcattggcga cctgcaccag tgccagcgca catgggacaa gatcttccac
     2821 aataccctca agatccacct cttctccatg gctttccggg agctgcagtg gctggtggcc
     2881 aagcgggtgc aggaccacac gacggttgtg ggtgatgtag tgtccccaga gatgggcgag
     2941 agtctgttcc agctctacat cagcctcaag gagctctgcc agctgcgcat gagctcctca
     3001 gagagggatg gagtcctggc cctggataat ttccaccgct ggttccagcc ggccatcccc
     3061 tcctggctgc agaagacgta caacgaggcc ctggcgcggg tgcagcgcgc tgtgcagatg
     3121 gatgagctgg tgcccctggg tgaactgacc aagcacagca catcagcggt ggatctatcc
     3181 acctgctttg cccagatcag ccacactgcc cggcagctgg actggccaga cccagaggag
     3241 gccttcatga ttaccgtcaa gtttgtggag gacacctgtc gcctggccct ggtgtactgc
     3301 agccttataa aggcccgggc ccgcgagctc tcttcaggcc agaaggacca aggccaggca
     3361 gccaacatgc tgtgtgtggt ggtgaatgac atggagcagc tgcggctggt gatcggcaag
     3421 ttgcccgccc agctggcatg ggaggccctg gagcagcggg taggggccgt gctggagcag
     3481 gggcagctgc agaacacgct gcatgcccag ctgcagagcg cgctggccgg gctgggccat
     3541 gagatccgca ctggcgtccg caccctggcc gagcagttgg aggtgggcat cgccaagcac
     3601 atccagaaac tggtgggcgt cagggagtct gtcctgcctg aggatgccat tctgcccctg
     3661 atgaagttcc tggaggtgga gctttgctac atgaacacca acttggtgca ggaaaacttc
     3721 agcagcctcc tgaccctgct ctggacccac acactcacag tgctggtgga ggcggccgcc
     3781 tcccagcgca gctcatccct ggcttccaac aggctgaaga ttgccctgca gaacctggag
     3841 atctgcttcc acgctgaggg ctgtggcctg ccacccgagg ccctgcacac tgccaccttc
     3901 caggctctgc agagggacct ggagctgcag gcggcctcca gccgggaact catccggaag
     3961 tacttctgca gccgaatcca gcagcaggca gaaaccacct ctgaggagct gggggctgtg
     4021 acagtcaagg cctcctaccg cgcctctgag cagaagctgc gtgtggagct gctcagcgcc
     4081 tccagcctgc tgcccctgga ctccaatggc tccagcgacc cctttgtcca gctgaccttg
     4141 gagcccaggc atgagttccc tgagctggcc gcccgggaga cccagaagca caagaaggac
     4201 cttcacccat tgtttgatga gacctttgaa ttcctggtgc ctgctgagcc gtgccgcaag
     4261 gctggggcat gcctcctgct caccgtgctg gactacgaca cgctgggggc cgacgacctg
     4321 gaaggcgagg ccttcctgcc gctgcgtgag gtgcccgggc tgagtggctc tgaggagcct
     4381 ggtgaggtgc ctcagacccg cctgcccctc acgtaccccg cacccaacgg ggacccaatc
     4441 ctgcagctgc tggagggccg gaagggtgac cgagaggccc aggtctttgt gaggctgcgg
     4501 cggcaccggg ccaagcaggc ctcccagcat gccttgcggc cggcaccgta agcgggcccg
     4561 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     4621 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     4681 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4741 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4801 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     4861 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     4921 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     4981 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     5041 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     5101 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     5161 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     5221 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     5281 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5341 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5401 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5461 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5521 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5581 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5641 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5701 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5761 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     5821 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     5881 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     5941 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     6001 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     6061 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     6121 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     6181 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     6241 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     6301 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     6361 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     6421 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     6481 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     6541 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     6601 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     6661 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     6721 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     6781 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     6841 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     6901 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     6961 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     7021 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     7081 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7141 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7201 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7261 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7321 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7381 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7441 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7501 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7561 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7621 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7681 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7741 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7801 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7861 ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt aatagtaatc
     7921 aattacgggg tcattagttc atagcccata tatggagttc cg
//
