LOCUS       sequence-488409-       11883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mitochondrial ATP biosensor, with miRNA-124 target sequence to 
            repress transgene expression in neurons .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11883)
  AUTHORS   Richetin K, Steullet P, Pachoud M, Perbet R, Parietti E, Maheswaran 
            M, Eddarkaoui S, Begard S, Pythoud C, Rey M, Caillierez R, Q Do K, 
            Halliez S, Bezzi P, Buee L, Leuba G, Colin M, Toni N, Deglon N
  TITLE     Tau accumulation in astrocytes of the dentate gyrus induces neuronal
            dysfunction and memory deficits in Alzheimer's disease.
  JOURNAL   Nat Neurosci. 2020 Dec;23(12):1567-1579. doi: 
            10.1038/s41593-020-00728-x. Epub 2020 Nov 9.
  PUBMED    33169029
REFERENCE   2  (bases 1 to 11883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-020-00728-x"; journalName: "Nat Neurosci"; date: 
            "2020-12"; volume: "23"; issue: "12"; pages: "1567-1579"
COMMENT     Sequence Label: SIN-cPPT-G1B3-mitoGO-ATeam2-WPRE-miR124T
FEATURES             Location/Qualifiers
     source          1..11883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2145
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3344..3368
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3451..3470
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3451..3469
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3516..3590
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             3612..3686
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      3723..3732
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3729..4382
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQFKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             4794..5528
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYIMADKQKNGIKVNFKIRHNIE"
     primer_bind     4931..4952
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5055..5076)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5316..5335)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(5576..5600)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5623..5639)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5624..5640)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    5653..6241
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5706..5726)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6124..6135)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6445..6678
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6703..6721)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6704..6721)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7011..7030)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7244..7263)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7363..7385
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7423..7441)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7509..7613
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7614..7682,7683..8474)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8645..9233
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9134..9153
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9387..9404
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9474..9494)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9479..9808
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9659..9794
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10942..11007
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11137..11157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11582..11716
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11619..11638)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11673..11692
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
     2041 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2101 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatccg atgtacgggc
     2161 cagatatacg cgttgacatt gattattgac taggatccgg taccgaattc gagctcggta
     2221 ccagatctaa catatcctgg tgtggagtag gggacgctgc tctgacagag gctcgggggc
     2281 ctgagctggc tctgtgagct ggggaggagg cagacagcca ggccttgtct gcaagcagac
     2341 ctggcagcat tgggctggcc gccccccagg gcctcctctt catgcccagt gaatgactca
     2401 ccttggcaca gacacaatgt tcggggtggg cacagtgcct gcttcccgcc gcaccccagc
     2461 ccccctcaaa tgccttccga gaagcccatt gagcaggggg cttgcattgc accccagcct
     2521 gacagcctgg catcttggga taaaagcagc acagccccct aggggctgcc cttgctgtgt
     2581 ggcgccaccg gcggtggaga acaaggctct attcagcctg tgcccaggaa aggggatcag
     2641 gggatgccca ggcatggaca gtgggtggca gggggggaga ggagggctgt ctgcttccca
     2701 gaagtccaag gacacaaatg ggtgagggga gagctctccc catagctggg ctgcggccca
     2761 accccacccc ctcaggctat gccagggggt gttgccaggg gcacccgatc ttcctcttca
     2821 tgcccagtga atgactcacc ttggcacaga cacaatgttc ggggtgggca cagtgcctgc
     2881 ttcccgccgc accccagccc ccctcaaatg ccttccgaga agcccattga gcaggcccga
     2941 tcttcctctt catgcccagt gaatgactca ccttggcaca gacacaatgt tcggggtggg
     3001 cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa tgccttccga gaagcccatt
     3061 gagcaggccc gatcttcctc ttcatgccca gtgaatgact caccttggca cagacacaat
     3121 gttcggggtg ggcacagtgc ctgcttcccg ccgcacccca gcccccctca aatgccttcc
     3181 gagaagccca ttgagcaggc ccgggcatcg ccagtctagc ccactccttc ataaagccct
     3241 cgcatcccag gagcgagcag agccagagca ggttggagag gagacgcatc acctccgctg
     3301 ctcgcgggga tcctagtgga tcccccgggc tgcaggaatt atcacaagtt tgtacaaaaa
     3361 agcaggctcc accatgggaa ccaattcagt cgactggatc tgagctctct ggctaactag
     3421 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
     3481 tggctagcgt ttaaacgggc cctctagact cgagcatgtc tgttctgact cctctgctgc
     3541 tccggggtct cacaggttcc gcaagaagac tccccgtgcc tagggccaaa attcattcac
     3601 tgggggaccc catgagcgtg ctcaccccac tcctgctgcg ggggctgacc ggcagcgcta
     3661 ggcggctgcc agtccccagg gccaagatcc acagtctcgg cgatcccaag gatccaccgg
     3721 tcgccaccat ggtgagtgtg attaaaccag agatgaagat gaggtactac atggacggct
     3781 ccgtcaatgg gcatgagttc acaattgaag gtgaaggcac aggcagacct tacgagggac
     3841 atcaagagat gacactacgc gtcacaatgg ccgagggcgg gccaatgcct ttcgcgtttg
     3901 acttagtgtc acacgtgttc tgttacggcc acagagtatt tactaaatat ccagaagaga
     3961 taccagacta tttcaaacaa gcatttcctg aaggcctgtc atgggaaagg tcgttggagt
     4021 tcgaagatgg tgggtccgct tcagtcagtg cgcatataag ccttagagga aacaccttct
     4081 accacaaatc caaatttact ggggttaact ttcctgccga tggtcctatc atgcaaaacc
     4141 aaagtgttga ttgggagcca tcaaccgaga aaattactgc cagcgacgga gttctgaagg
     4201 gtgatgttac gatgtaccta aaacttgaag gaggcggcaa tcacaaatgc caattcaaga
     4261 ctacttacaa ggcggcaaaa gagattcttg aaatgccagg agaccattac atcggccatc
     4321 gcctcgtcag gaaaaccgaa ggcaacatta ctgagcaggt agaagatgca gtagctcatt
     4381 ccatcgatat gaaaactgtg aaagtgaata taacaacccc tgatgggcca gtctacgacg
     4441 ctgatatcga gatggtgtcc gtgcgggccg agagtggtga tctcggcatc ctccccggtc
     4501 acattcccac aaaggcccca ctgaagatcg gagctgtgcg gctgaagaag gacggccaaa
     4561 ccgagtacgt cgcagtctca ggcggcactg ttgaagtgcg gcctgaccac gttaccatta
     4621 atgctcaagc cgctgaaaca gccgaaggaa tcgacaaaga gagagcagaa gccgcaagac
     4681 agagggccca ggagcggctg aactctcaat ccgatgacac cgatattcgc cgggccgagc
     4741 tggcactgca gagggccctg aacagactgg acgtggctga aatgaaggaa ttcatggacg
     4801 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4861 tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga
     4921 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4981 acgagctgta caagggtggc agcggtggca tggtgagcaa gggcgaggag ctgttcaccg
     5041 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     5101 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     5161 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     5221 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     5281 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     5341 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     5401 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     5461 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     5521 acatcgagtg aagctaattc gcggccgcac tcgagatgga agatcttcca tctagaccca
     5581 gctttcttgt acaaagtggt gataattcga tatcaagctt atcgataccg tcgacctcga
     5641 gggaattccg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     5701 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     5761 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     5821 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     5881 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     5941 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6001 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     6061 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6121 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6181 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6241 catcgggaat tcgagctcgg tacctaatgg cattcaccgc gtgccttaat tcgaatggca
     6301 ttcaccgcgt gccttaaacg cgttggcatt caccgcgtgc cttaaatgca ttggcattca
     6361 ccgcgtgcct taaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6421 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     6481 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6541 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6601 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6661 tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa
     6721 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat
     6781 gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta
     6841 aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc
     6901 gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc
     6961 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     7021 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     7081 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     7141 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     7201 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     7261 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     7321 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     7381 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     7441 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     7501 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7561 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7621 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7681 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7741 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7801 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7861 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7921 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7981 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8041 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8101 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8161 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8221 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8281 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8341 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8401 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8461 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8521 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8581 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8641 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8701 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8761 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8821 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8881 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8941 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9001 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9061 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9301 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9361 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9421 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     9481 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     9541 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     9601 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     9661 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     9721 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9781 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     9841 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     9901 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     9961 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
    10021 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
    10081 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
    10141 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
    10201 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
    10261 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
    10321 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
    10381 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
    10441 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
    10501 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
    10561 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
    10621 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
    10681 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
    10741 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
    10801 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
    10861 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
    10921 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
    10981 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
    11041 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
    11101 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
    11161 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
    11221 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
    11281 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
    11341 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
    11401 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
    11461 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
    11521 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    11581 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    11641 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    11701 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    11761 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
    11821 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
    11881 agt
//
