LOCUS       sequence-488451-        9401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human ASB8 protein with an N-terminal Tag100 along with a 
            puromycin selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Wing CE, Fung HYJ, Kwanten B, Cagatay T, Niesman AB, Jacquemyn M, 
            Gharghabi M, Permentier B, Shakya B, Nandi R, Ready JM, Kashyap T, 
            Shacham S, Landesman Y, Lapalombella R, Daelemans D, Chook YM
  TITLE     SINE compounds activate exportin 1 degradation through an allosteric
            mechanism.
  JOURNAL   Nat Chem Biol. 2025 Dec;21(12):2002-2013. doi: 
            10.1038/s41589-025-02058-0. Epub 2025 Nov 21.
  PUBMED    41286136
REFERENCE   2  (bases 1 to 9401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-025-02058-0"; journalName: "Nat Chem Biol"; date: 
            "2025-12"; volume: "21"; issue: "12"; pages: "2002-2013"
COMMENT     Sequence Label: pLV-puro-EF1A-Tag100-hASB8
FEATURES             Location/Qualifiers
     source          1..9401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1959..3137
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2190..3128
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3085..3105
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3137..3161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3162..3171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3171..3206
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
     protein_bind    complement(4074..4098)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4116..4704
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4169..4189)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4587..4598)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4735..5234
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4770..4793)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5167..5186
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5251..5850
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5251..5270)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5747..5767
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5922..6155
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6227..6348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6264..6283)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6318..6337
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6388..6523
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6543..6562)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6544..6562)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6572..6589)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6572..6588)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6581..6603)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6730..7185
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6817..6836)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7027..7048
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7211..7315
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7316..7384,7385..8176)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7534..7553)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8347..8935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8836..8855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9089..9106
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9223..9244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9259..9264,9265..9282,9283..9289)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9297..9313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9302..9324
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9321..9337
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9321..9337
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9356..9376
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9358..9376
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg gaagaaacag
     3181 cgaggttcca gcccggttac cggagtatga gttccagtat gtggtatatt atgcagagca
     3241 ttcagagcaa atactctctc tccgagcgct taatccgaac aattgctgcc atccgttcct
     3301 tcccacatga taatgtagag gacctcatca gagggggagc agatgtgaac tgcactcatg
     3361 gcacactgaa gcccttgcac tgtgcctgta tggtgtcaga tgctgactgt gtggagttac
     3421 ttctggaaaa aggagccgag gtgaatgccc tggatgggta taaccgaaca gccctccact
     3481 atgcagcaga gaaagatgag gcttgtgtgg aggtcctatt ggagtatggt gcaaacccca
     3541 atgctttgga tggcaacaga gataccccac ttcactgggc agcctttaag aacaatgctg
     3601 agtgtgtgcg ggctctccta gagagcgggg cctctgtcaa tgccctggat tacaacaatg
     3661 atacaccgct cagctgggct gccatgaagg gaaatcttga gagtgtcagc atccttctgg
     3721 attatggcgc agaggtcaga gtcatcaacc taataggcca gacacccatc tcccgcctgg
     3781 tggctctgct agtcagggga cttggaacag agaaagagga ctcttgcttt gagctcctcc
     3841 acagagctgt tggacacttt gaattgagga aaaatggcac catgccacga gaggtggcca
     3901 gagacccgca gctatgtgaa aaactgactg ttctgtgctc agctccagga actctaaaaa
     3961 cactcgctcg ctatgccgtg cgccgtagcc tgggactcca gtatctcccc gatgcagtga
     4021 agggccttcc actgccagct tctttgaagg aatacctgtt acttttagaa tagacccagc
     4081 tttcttgtac aaagtggtga taatcgaatt ccgataatca acctctggat tacaaaattt
     4141 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4201 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4261 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4321 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4381 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4441 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4501 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     4561 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4621 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4681 tctccctttg ggccgcctcc ccgcatcggg aattcccgcg gttcgaattc taccgggtag
     4741 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     4801 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     4861 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     4921 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     4981 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     5041 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     5101 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     5161 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     5221 ctccgggcct ttcgacctca cgtggccacc atgaccgagt acaagcccac ggtgcgcctc
     5281 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     5341 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     5401 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     5461 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     5521 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     5581 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     5641 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     5701 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     5761 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     5821 tggtgcatga cccgcaagcc cggtgcctga ggtaccttta agaccaatga cttacaaggc
     5881 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5941 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     6001 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6061 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6121 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     6181 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     6241 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6301 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     6361 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6421 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     6481 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     6541 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     6601 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcaccagct
     6661 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     6721 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6781 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6841 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6901 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6961 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     7021 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     7081 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     7141 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     7201 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7261 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7321 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7381 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7441 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7501 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7561 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7621 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7681 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7741 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7801 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7861 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7921 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7981 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     8041 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8101 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8161 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8221 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8281 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8341 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8401 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8461 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8521 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8581 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8641 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8701 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8761 cgaagagaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8821 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8881 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8941 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9001 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9061 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9121 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     9181 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     9241 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     9301 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     9361 taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
