LOCUS       sequence-488463-        2531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tscA-chlN IEE5 Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3IEE_023_IEE5
FEATURES             Location/Qualifiers
     source          1..2531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      475..532
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(556..573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(727..1315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(807..826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1498..1592)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1613..2272)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2187..2206
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2434..2452
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2456..2499)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2502..2523
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgtac tgacttgtaa aaatatgacg tctttttcct ttggtttata gtatttggaa
       61 aagcaaaatg tttagacatc tggtttaaag acagtatact acttgattgc acttttggcc
      121 gtatagtaga tcataggtta aaaggctatt tgtacataag tagactttta cattttaatt
      181 tttttgtaag tttgaataca tttagttaat taagttttag atttttaaat taaaaagctc
      241 ccttgtatga ggaagtaggt tgtcccacag ggattgctta aaatctaaca aaaatacatt
      301 tccgttaaat taagatgggt gtagcatctc gctgctatac ttcaccaaaa gatcttactt
      361 ccacataccc tgtttgtgac caagtaagtg tttactttta gatttaacgt gtttataaca
      421 tttaagtaat tataatgtga gaccactcat cgcgactagt agcggccgct gcagtccggc
      481 aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
      541 cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      601 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      661 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      721 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      781 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      841 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      901 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      961 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1021 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1081 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1141 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1201 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1261 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1321 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1381 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1441 ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt
     1501 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     1561 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
     1621 cgccctgcca ctcgtcgcag tactgttgta attcattaag cattctgccg acatggaagc
     1681 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     1741 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     1801 aatcaaaact ggtgaaactc acccagggat tggctgatac gaaaaacata ttctcaataa
     1861 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     1921 gtagaaactg ccggaaatcg tcgtggtatt cactccagag tgatgaaaac gtttcagttt
     1981 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     2041 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     2101 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     2161 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     2221 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     2281 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     2341 tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa
     2401 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc
     2461 agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta
     2521 gagcgatgag t
//
