LOCUS       sequence-488465-        9431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human ASB8 protein with an N-terminal 3xFLAG tag along 
            with a puromycin selection marker. The L199G mutation disrupts XPO1 
            binding..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Wing CE, Fung HYJ, Kwanten B, Cagatay T, Niesman AB, Jacquemyn M, 
            Gharghabi M, Permentier B, Shakya B, Nandi R, Ready JM, Kashyap T, 
            Shacham S, Landesman Y, Lapalombella R, Daelemans D, Chook YM
  TITLE     SINE compounds activate exportin 1 degradation through an allosteric
            mechanism.
  JOURNAL   Nat Chem Biol. 2025 Dec;21(12):2002-2013. doi: 
            10.1038/s41589-025-02058-0. Epub 2025 Nov 21.
  PUBMED    41286136
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-025-02058-0"; journalName: "Nat Chem Biol"; date: 
            "2025-12"; volume: "21"; issue: "12"; pages: "2002-2013"
COMMENT     Sequence Label: pLV-puro-EF1A-3XFLAG-hASB8_L199G
FEATURES             Location/Qualifiers
     source          1..9431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1959..3137
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2190..3128
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3085..3105
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3137..3161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3162..3171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3171..3236
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(4104..4128)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4146..4734
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4199..4219)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4617..4628)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4765..5264
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4800..4823)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5197..5216
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5281..5880
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5281..5300)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5777..5797
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5952..6185
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6257..6378
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6294..6313)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6348..6367
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6418..6553
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6573..6592)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6574..6592)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6602..6619)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6602..6618)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6611..6633)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6760..7215
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6847..6866)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7057..7078
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7241..7345
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7346..7414,7415..8206)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7564..7583)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8377..8965
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8866..8885
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9119..9136
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9253..9274
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9289..9294,9295..9312,9313..9319)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9327..9343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9332..9354
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9351..9367
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9351..9367
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9386..9406
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9388..9406
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg gactacaaag
     3181 accatgacgg tgattataaa gatcatgata tcgattacaa ggatgacgat gacaagatga
     3241 gttccagtat gtggtatatt atgcagagca ttcagagcaa atactctctc tccgagcgct
     3301 taatccgaac aattgctgcc atccgttcct tcccacatga taatgtagag gacctcatca
     3361 gagggggagc agatgtgaac tgcactcatg gcacactgaa gcccttgcac tgtgcctgta
     3421 tggtgtcaga tgctgactgt gtggagttac ttctggaaaa aggagccgag gtgaatgccc
     3481 tggatgggta taaccgaaca gccctccact atgcagcaga gaaagatgag gcttgtgtgg
     3541 aggtcctatt ggagtatggt gcaaacccca atgctttgga tggcaacaga gataccccac
     3601 ttcactgggc agcctttaag aacaatgctg agtgtgtgcg ggctctccta gagagcgggg
     3661 cctctgtcaa tgccctggat tacaacaatg atacaccgct cagctgggct gccatgaagg
     3721 gaaatcttga gagtgtcagc atccttctgg attatggcgc agaggtcaga gtcatcaacc
     3781 taataggcca gacacccatc tcccgcctgg tggctctgct agtcagggga ggtggaacag
     3841 agaaagagga ctcttgcttt gagctcctcc acagagctgt tggacacttt gaattgagga
     3901 aaaatggcac catgccacga gaggtggcca gagacccgca gctatgtgaa aaactgactg
     3961 ttctgtgctc agctccagga actctaaaaa cactcgctcg ctatgccgtg cgccgtagcc
     4021 tgggactcca gtatctcccc gatgcagtga agggccttcc actgccagct tctttgaagg
     4081 aatacctgtt acttttagaa tagacccagc tttcttgtac aaagtggtga taatcgaatt
     4141 ccgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4201 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4261 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4321 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4381 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4441 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4501 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     4561 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4621 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4681 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcggg
     4741 aattcccgcg gttcgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     4801 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     4861 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     4921 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     4981 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     5041 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     5101 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     5161 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     5221 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctca cgtggccacc
     5281 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     5341 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     5401 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     5461 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     5521 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     5581 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     5641 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     5701 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     5761 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     5821 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     5881 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5941 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     6001 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6061 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6121 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6181 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     6241 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6301 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6361 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     6421 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6481 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     6541 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     6601 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     6661 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     6721 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     6781 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6841 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6901 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6961 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     7021 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     7081 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     7141 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     7201 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     7261 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7321 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     7381 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7441 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7501 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     7561 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     7621 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     7681 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     7741 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7801 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7861 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7921 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7981 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     8041 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     8101 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     8161 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     8221 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     8281 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     8341 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8401 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8461 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8521 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8581 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8641 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8701 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8761 gcgtgagcta tgagaaagcg ccacgcttcc cgaagagaga aaggcggaca ggtatccggt
     8821 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8881 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8941 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     9001 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     9061 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     9121 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     9181 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     9241 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     9301 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     9361 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     9421 gctgcaagct t
//
