LOCUS       sequence-488475-        2200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psbF 5'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3_009_psbF_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      144..201
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(225..242)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(396..984)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(476..495)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1167..1261)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1282..1941)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1856..1875
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2103..2121
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2125..2168)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2171..2192
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgtac tattatattt attttaaact aatatttaca taaattttaa acgagttagc
       61 ttaatacaaa aggtaaaatt accctattta tttttattar ttaatgtgag accactcatc
      121 gcgactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc accctgccct
      181 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
      241 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      301 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      361 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
      421 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      481 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      541 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      601 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      661 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      721 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      781 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      841 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      901 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      961 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1021 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1081 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1141 acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     1201 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     1261 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcgtcgcagt actgttgtaa
     1321 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     1381 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     1441 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     1501 ggctgatacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     1561 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     1621 actccagagt gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     1681 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
     1741 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     1801 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     1861 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     1921 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     1981 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc
     2041 gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     2101 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
     2161 atgatttctg gaattcgcgg ccgcttctag agcgatgagt
//
