LOCUS       sequence-488543-       16039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector expresses FLAG-tagged full length human PIWIL2 
            (HILI).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16039)
  AUTHORS   Risner A, Nair-Menon J, Cheedipudi A, Delaney JR, Gangaraju V, 
            Kourtidis A
  TITLE     PIWIL2 downregulation in colon cancer promotes transposon activity 
            and pro-tumorigenic phenotypes.
  JOURNAL   Biol Open. 2025 Aug 11:bio.061942. doi: 10.1242/bio.061942.
  PUBMED    40789164
REFERENCE   2  (bases 1 to 16039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Open. 
            2025 Aug 11:bio.061942. doi: 10.1242/bio.061942."
COMMENT     Sequence Label: LZRS-MS-NEO_PIWIL2-FLAG
FEATURES             Location/Qualifiers
     source          1..16039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             514..983
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1048..1405
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1470..1886
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1816..1838
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1854..1870
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1896..2270
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2208..2224
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             4005..4016
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(4921..4932)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5238..5309
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     misc_feature    5398..5969
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5563..5580)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5790..5809
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6005..6808
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6068..6087)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6678..6697
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             7026..7495
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7625..7642)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7796..8384)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7876..7895)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    8507..8641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8531..8550)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8585..8604
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8871..9470)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8954..8974)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9451..9470
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(9553..10052)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9601..9620)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     9994..10017
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             10481..12406
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     CDS             complement(join(15051..15842,15843..15911))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15674..15693
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15912..16016)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cagctttgct cttaggagtt tcctaataca tcccaaactc aaatatataa agcatttgac
       61 ttgttctatg ccctaggggg cggggggaag ctaagccagc tttttttaac atttaaaatg
      121 ttaattccat tttaaatgca cagatgtttt tatttcataa gggtttcaat gtgcatgaat
      181 gctgcaatat tcctgttacc aaagctagta taaataaaaa tagataaacg tggaaattac
      241 ttagagtttc tgtcattaac gtttccttcc tcagttgaca acataaatgc gctgctgaga
      301 agccagtttg catctgtcag gatcaatttc ccattatgcc agtcatatta attactagtc
      361 aattagttga tttttatttt tgacatatac atgtgaaaga ccccacctgt aggtttggca
      421 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa
      481 agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg
      541 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc
      601 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
      661 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
      721 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
      781 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc
      841 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
      901 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
      961 tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca
     1021 gggaccaccg acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga
     1081 ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct
     1141 ctgtatctgg cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg
     1201 gagacgtccc agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg
     1261 atcgttttgg actctttggt gcacccccct tagaggaggg atatgtggtt ctggtaggag
     1321 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa
     1381 gccgcgccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt
     1441 ttctgtattt gtctgaaaat atgggcccgg gccagactgt taccactccc ttaagtttga
     1501 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
     1561 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
     1621 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
     1681 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
     1741 acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat
     1801 ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc
     1861 cagccctcac tccttctcta ggcgccccca tatggccata tgagatctta tatggggcac
     1921 ccccgcccct tgtaaacttc cctgaccctg acatgacaag agttactaac agcccctctc
     1981 tccaagctca cttacaggct ctctacttag tccagcacga agtctggaga cctctggcgg
     2041 cagcctacca agaacaactg gaccgaccgg tggtacctca cccttaccga gtcggcgaca
     2101 cagtgtgggt ccgccgacac cagactaaga acctagaacc tcgctggaaa ggaccttaca
     2161 cagtcctgct gaccaccccc accgccctca aagtagacgg catcgcagct tggatacacg
     2221 ccgcccacgt gaaggctgcc gaccccgggg gtggaccatc ctctagactg ccggatccca
     2281 gtgtggtggt agggaattca aacgggccct ctagaccaat ggatcctttc cgaccatcgt
     2341 tcaggggcca gtctcctatc cacccatccc agtgccaggc tgtacggatg ccaggctgtt
     2401 ggccacaagc ttctaaacct ttggacccag ctctgggcag gggagcacct gcaggcagag
     2461 gccatgtatt tggaaagcca gaggaaccaa gcacacagag ggggccagca caaagggagt
     2521 ctgtgggttt ggtctccatg ttccgaggcc tgggcattga aacagtttct aagacccctc
     2581 tgaaacggga aatgcttcca tcaggtagag gcattttagg tcgaggcttg tctgctaatc
     2641 tggtacgcaa ggacagggag gaactctctc ccactttttg ggatccaaaa gtgttggcgg
     2701 ctggggacag caagatggca gagacctccg ttggttggag taggacgctt ggaagaggga
     2761 gttcagatgc gtctttatta ccactgggaa gagcagcagg tggtatcagc agagaagtgg
     2821 acaagcctcc ctgtaccttc agcacaccgt cccggggtcc cccgcagctg tcatcaccac
     2881 cagctctgcc ccagtctccc ctgcactctc cagatcgccc tctggtcctg actgtggaac
     2941 acaaggaaaa agagcttatt gtgaagcaag gatcaaaagg aacacctcag tctttgggac
     3001 tgaacctcgt caaaatacag tgtcataatg aagcagttta tcaatatcat gtgactttca
     3061 gccccaatgt ggagtgcaaa agcatgaggt tcggcatgtt gaaggaccat caagctgtca
     3121 ccggcaacgt cactgcgttt gatggatcta ttctctatct gcctgttaag cttcaacaag
     3181 ttcttgagtt aaaaagtcaa aggaaaacag acagtgctga aatcagcatt aagattcaga
     3241 tgacaaagat cctggagccc tgctctgacc tgtgcattcc cttctacaat gttgttttcc
     3301 gtcgggtaat gaaactttta gatatgaagc ttgtggggag aaacttttat gaccctacaa
     3361 gtgctatggt actacagcaa cacagattgc agatctggcc aggctatgca gctagcatcc
     3421 gaaggacaga tggagggctc ttcctgctag ctgatgtctc ccataaggtc attcggaatg
     3481 actgtgtgct ggatgtcatg catgccattt atcagcagaa taaagaacac ttccaggatg
     3541 agtgtactaa gcttctggtt ggcaatattg ttatcacccg atataacaat cgtacctatc
     3601 gtattgatga tgtggattgg aataagactc caaaggatag cttcacgatg tctgatggga
     3661 aagagatcac attcttggaa tactacagca aaaattatgg gatcacagtt aaggaagagg
     3721 accagccatt gctgattcac aggcccagtg agagacagga taatcatggg atgctgctaa
     3781 aaggggaaat cctgctgctg cctgagcttt cttttatgac cggaatccca gagaagatga
     3841 agaaggactt cagagccatg aaggatttgg ctcagcaaat caatctgagc cccaagcaac
     3901 accatagtgc tttggaatgc ttgctgcaaa gaattgcaaa gaacgaggca gccaccaatg
     3961 aactgatgcg ttgggggctc cgtctgcaaa aggatgtaca taagattgaa ggacgtgttc
     4021 tgccaatgga aagaattaac ttaaaaaata cttcgtttat cacatctcag gaactaaact
     4081 gggttaagga agtaaccaga gacccttcca tcttgactat ccccatgcat ttctgggcac
     4141 ttttttaccc aaagagagca atggaccagg ctcgagaact ggtcaacatg ttggagaaga
     4201 tagccggccc cattggcatg cgtatgagcc caccggcctg ggttgaacta aaggatgacc
     4261 gaatagagac ttatgtcaga accattcaat ccacgttagg agctgagggg aagatacaga
     4321 tggttgtttg catcatcatg ggcccacgtg atgatctcta tggggccatc aagaagctgt
     4381 gctgtgtgca gtccccagtg ccctcccagg ttgtcaatgt tcgaaccatt ggtcagccca
     4441 ccaggcttcg gagtgtggcc cagaagattt tacttcagat taactgtaaa ttgggtggtg
     4501 agctctgggg agtggatatt cctctgaaac agttaatggt gatcgggatg gatgtttacc
     4561 atgaccccag tagaggcatg cgctccgtgg ttggcttcgt ggcaagcatc aatctcaccc
     4621 tcacaaaatg gtattcccgg gtggtgttcc agatgccgca tcaggagatt gtggacagcc
     4681 tgaagctatg cctcgtgggc tccttaaaaa agttttatga ggtgaaccac tgtctaccag
     4741 agaagattgt ggtgtaccgt gatggagtgt ctgatggcca actgaagaca gttgccaact
     4801 atgagattcc tcaactacag aagtgttttg aagcttttga gaattatcag cccaagatgg
     4861 tggtgtttgt agttcagaag aaaatcagta ctaatctata tctggctgct cctcagaact
     4921 ttgtaactcc cactcctgga actgtggtag atcatacaat aacaagctgt gagtgggtgg
     4981 atttctatct tcttgcccat catgtacggc agggctgtgg cattcctacg cattatgtct
     5041 gtgttctcaa caccgcaaac ctgagccctg atcatatgca gaggctgact ttcaaactgt
     5101 gccacatgta ctggaattgg cctggcacca tcagagttcc agctccttgc aagtatgccc
     5161 acaagctagc tttcctgtca ggacacatct tgcatcatga accagccatc cagctgtgcg
     5221 agaacctgtt cttcctggat tacaaggatg acgacgataa ggactataag gacgatgatg
     5281 acaaggacta caaagatgat gacgataaat gagaattctg caagccgcga tcgcagcgct
     5341 ggccggtccg gccggtccaa ctgcaggtcg agcatgcatc tagggcggcc aattccgccc
     5401 ctctcccccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     5461 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     5521 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     5581 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     5641 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     5701 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     5761 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     5821 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     5881 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     5941 tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccgggataa ttcctgcagc
     6001 caatatggga tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     6061 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     6121 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     6181 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     6241 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     6301 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     6361 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     6421 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     6481 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     6541 gcgcatgccc gacggcgatg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     6601 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     6661 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     6721 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     6781 ctatcgcctt cttgacgagt tcttctgagg ggatcaattc tctagagctc gctgatcgcg
     6841 gccgccagca cagtggtcga cgataaaata aaagatttta tttagtctcc agaaaaaggg
     6901 gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa
     6961 ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag gaacagatgg
     7021 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     7081 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     7141 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
     7201 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     7261 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     7321 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
     7381 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     7441 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcacatgc
     7501 agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt
     7561 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7621 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7681 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7741 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7801 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7861 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7921 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7981 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8041 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8101 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8161 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8221 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8281 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8341 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8401 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8461 gattatcaaa aaggatcttc acctagatcc ttttgcggcc gaattagatc cagacatgat
     8521 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     8581 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     8641 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     8701 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc tagagtcggt
     8761 gggcctcggg ggcgggtgcg gggtcggcgg ggccgccccg ggtggcttcg gtcggagcca
     8821 tggggtcgtg cgctcctttc ggtcgggcgc tgcgggtcgt ggggcgggcg tcaggcaccg
     8881 ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg
     8941 gtgaagccga gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg
     9001 ggcaccccgg cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc
     9061 ttgccctggt ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc
     9121 cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac
     9181 tcggccatgc gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg
     9241 gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg
     9301 acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccgggtcg
     9361 acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggcccgg
     9421 gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat ggaaggtcgt
     9481 ctccttgtga ggggtcaggg gcgtgggtca ggggatggtg gcggcaccgg tcgtggcggc
     9541 cgacctgcag gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg cagacgtgcg
     9601 cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct
     9661 gagcccgccc ctgagcccgc ccccggaccc accccttccc agcctctgag cccagaaagc
     9721 gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct acccgcttcc attgctcagc
     9781 ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc acgtcctgca
     9841 cgacgcgagc tgcggggcgg gggggaactt cctgactagg ggaggagtag aaggtggcgc
     9901 gaaggggcca ccaaagaacg gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg
     9961 aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag cgcatgctcc
    10021 agactgcctt gggaaaagcg cctcccctac ccggtagaat taattctcat gtttgacagc
    10081 ttatcatcga taagctgatc ctcacaggcc gcacccagct tttcttccgt tgccccagta
    10141 gcatctctgt ctggtgacct tgaagaggaa gaggaggggt cccgagaatc cccatcccta
    10201 ccgtccagca aaaaggggga cgaggaattt gaggcctggc ttgaggctca ggacgcaaat
    10261 cttgaggatg ttcagcggga gttttccggg ctgcgagtaa ttggtgatga ggacgaggat
    10321 ggttcggagg atggggaatt ttcagacctg gatctgtctg acagcgacca tgaaggggat
    10381 gagggtgggg gggctgttgg agggggcagg agtctgcact ccctgtattc actgagcgtc
    10441 gtctaataaa gatgtctatt gatctctttt agtgtgaatc atgtctgacg aggggccagg
    10501 tacaggacct ggaaatggcc taggagagaa gggagacaca tctggaccag aaggctccgg
    10561 cggcagtgga cctcaaagaa gagggggtga taaccatgga cgaggacggg gaagaggacg
    10621 aggacgagga ggcggaagac caggagcccc gggcggctca ggatcagggc caagacatag
    10681 agatggtgtc cggagacccc aaaaacgtcc aagttgcatt ggctgcaaag ggacccacgg
    10741 tggaacagga gcaggagcag gagcgggagg ggcaggagca ggaggggcag gagcaggagg
    10801 aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagggg caggagcagg
    10861 aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag caggaggagg
    10921 ggcaggagca ggaggagggg caggaggggc aggagcagga ggaggggcag gaggggcagg
    10981 aggggcagga gcaggaggag gggcaggagc aggaggaggg gcaggagggg caggagcagg
    11041 aggaggggca ggaggggcag gaggggcagg agcaggagga ggggcaggag caggaggggc
    11101 aggaggggca ggaggggcag gagcaggagg ggcaggagca ggaggagggg caggaggggc
    11161 aggaggggca ggagcaggag gggcaggagc aggaggggca ggagcaggag gggcaggagc
    11221 aggaggggca ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggggcagg
    11281 aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggagggg caggaggggc
    11341 aggagcagga ggggcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg
    11401 ggcaggaggg gcaggagcag gaggaggggc aggagcagga ggggcaggag caggaggtgg
    11461 aggccggggt cgaggaggca gtggaggccg gggtcgagga ggtagtggag gccggggtcg
    11521 aggaggtagt ggaggccgcc ggggtagagg acgtgaaaga gccagggggg gaagtcgtga
    11581 aagagccagg gggagaggtc gtggacgtgg agaaaagagg cccaggagtc ccagtagtca
    11641 gtcatcatca tccgggtctc caccgcgcag gccccctcca ggtagaaggc catttttcca
    11701 ccctgtaggg gaagccgatt attttgaata ccaccaagaa ggtggcccag atggtgagcc
    11761 tgacgtgccc ccgggagcga tagagcaggg ccccgcagat gacccaggag aaggcccaag
    11821 cactggaccc cggggtcagg gtgatggagg caggcgcaaa aaaggagggt ggtttggaaa
    11881 gcatcgtggt caaggaggtt ccaacccgaa atttgagaac attgcagaag gtttaagagc
    11941 tctcctggct aggagtcacg tagaaaggac taccgacgaa ggaacttggg tcgccggtgt
    12001 gttcgtatat ggaggtagta agacctccct ttacaaccta aggcgaggaa ctgcccttgc
    12061 tattccacaa tgtcgtctta caccattgag tcgtctcccc tttggaatgg cccctggacc
    12121 cggcccacaa cctggcccgc taagggagtc cattgtctgt tatttcatgg tctttttaca
    12181 aactcatata tttgctgagg ttttgaagga tgcgattaag gaccttgtta tgacaaagcc
    12241 cgctcctacc tgcaatatca gggtgactgt gtgcagcttt gacgatggag tagatttgcc
    12301 tccctggttt ccacctatgg tggaaggggc tgccgcggag ggtgatgacg gagatgacgg
    12361 agatgaagga ggtgatggag atgagggtga ggaagggcag gagtgatgta acttgttagg
    12421 agacgccctc aatcgtatta aaagccgtgt attcccccgc actaaagaat aaatccccag
    12481 tagacatcat gcgtgctgtt ggtgtatttc tggccatctg tcttgtcacc attttcgtcc
    12541 tcccaacatg gggcaattgg gcatacccat gttgtcacgt cactcagctc cgcgctcaac
    12601 accttctcgc gttggaaaac attagcgaca tttacctggt gagcaatcag acatgcgacg
    12661 gctttagcct ggcctcctta aattcaccta agaatgggag caaccagcag gaaaaggaca
    12721 agcagcgaaa attcacgccc ccttgggagg tggcggcata tgcaaaggat agcactccca
    12781 ctctactact gggtatcata tgctgactgt atatgcatga ggatagcata tgctacccgg
    12841 atacagatta ggatagcata tactacccag atatagatta ggatagcata tgctacccag
    12901 atatagatta ggatagccta tgctacccag atataaatta ggatagcata tactacccag
    12961 atatagatta ggatagcata tgctacccag atatagatta ggatagccta tgctacccag
    13021 atatagatta ggatagcata tgctacccag atatagatta ggatagcata tgctatccag
    13081 atatttgggt agtatatgct acccagatat aaattaggat agcatatact accctaatct
    13141 ctattaggat agcatatgct acccggatac agattaggat agcatatact accctaatct
    13201 ctattaggat agcatatgct acccggatac agattaggat agcatatact acccagatat
    13261 agattaggat agcatatgct acccagatat agattaggat agcctatgct acccagatat
    13321 aaattaggat agcatatact acccagatat agattaggat agcatatgct acccagatat
    13381 agattaggat agcctatgct acccagatat agattaggat agcatatgct atccagatat
    13441 ttgggtagta tatgctaccc atggcaacat tagcccaccg tgctctcagc gacctcgtga
    13501 atatgaggac caacaaccct gtgcttggcg ctcaggcgca agtgtgtgta atttgtcctc
    13561 cagatcgcag caatcgcgcc cctatcttgg cccgcccacc tacttatgca ggtattcccc
    13621 ggggtgccat tagtggtttt gtgggcaagt ggtttgaccg cagtggttag cggggttaca
    13681 atcagccaag ttattacacc cttattttac agtccaaaac cgcagggcgg cgtgtggggg
    13741 ctgacgcgtg cccccactcc acaatttcaa aaaaaagagt ggccacttgt ctttgtttat
    13801 gggccccatt ggcgtggagc cccgtttaat tttcgggggt gttagagaca accagtggag
    13861 tccgctgctg tcggcgtcca ctctctttcc ccttgttaca aatagagtgt aacaacatgg
    13921 ttcacctgtc ttggtccctg cctgggacac atcttaataa ccccagtatc atattgcact
    13981 aggattatgt gttgcccata gccataaatt cgtgtgagat ggacatccag tctttacggc
    14041 ttgtccccac cccatggatt tctattgtta aagatattca gaatgtttca ttcctacact
    14101 agtatttatt gcccaagggg tttgtgaggg ttatattggt gtcatagcac aatgccacca
    14161 ctgaaccccc cgtccaaatt ttattctggg ggcgtcacct gaaaccttgt tttcgagcac
    14221 ctcacataca ccttactgtt cacaactcag cagttattct attagctaaa cgaaggagaa
    14281 tgaagaagca ggcgaagatt caggagagtt cactgcccgc tccttgatct tcagccactg
    14341 cccttgtgac taaaatggtt cactaccctc gtggaatcct gaccccatgt aaataaaacc
    14401 gtgacagctc atggggtggg agatatcgct gttccttagg acccttttac taaccctaat
    14461 tcgatagcat atgcttcccg ttgggtaaca tatgctattg aattagggtt agtctggata
    14521 gtatatacta ctacccggga agcatatgct acccgtttag ggttaacaag ggggccttat
    14581 aaacactatt gctaatgccc tcttgagggt ccgcttatcg gtagctacac aggcccctct
    14641 gattgacgtt ggtgtagcct cccgtagtct tcctgggccc ctgggaggta catgtccccc
    14701 agcattggtg taagagcttc agccaagagt tacacataaa ggcaatgttg tgttgcagtc
    14761 cacagactgc aaagtctgct ccaggatgaa agccactcag tgttggcaaa tgtgcacatc
    14821 catttataag gatgtcaact acagtcagag aacccctttg tgtttggtcc ccccccgtgt
    14881 cacatgtgga acagggccca gttggcaagt tgtaccaacc aactgaaggg attacatgca
    14941 ctgccccgcg aagaaggggc agagatgtcg tagtcaggtt tagttcgtcc ggggcgggga
    15001 tctggccgca aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    15061 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    15121 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    15181 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    15241 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    15301 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    15361 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    15421 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    15481 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    15541 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    15601 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    15661 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    15721 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    15781 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    15841 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    15901 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    15961 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    16021 tttccccgaa aagtgccac
//
