LOCUS       sequence-488560-        4917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of MBP-JARID2 in insect cells, MBP tags are C3-cleavable 
            (PreScission Protease).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4917)
  TITLE     Davidovich Lab AEBP Plasmids 2025
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMB.MBP.JARID2
FEATURES             Location/Qualifiers
     source          1..4917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..1107
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      964..973
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1081..1104
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1141..1164
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     RBS             2427..2435
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgggtacca aaactgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat
       61 aacggtttgg ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt
      121 gagcatccgg ataaactgga agagaaattc ccacaggttg cggcaactgg cgatggccct
      181 gacattatct tctgggcaca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct
      241 gaaatcaccc cggacaaagc gttccaggac aagctgtatc cgtttacctg ggatgccgta
      301 cgttacaacg gcaagctgat tgcttacccg atcgctgttg aagcgttatc gctgatttat
      361 aacaaagatt tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa
      421 gaactgaaag cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc
      481 tggccgctga ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac
      541 attaaagacg tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac
      601 ctgattaaaa acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt
      661 aataaaggcg aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc
      721 agcaaagtga attatggtgt aacggtactg ccgaccttca agggtcaacc atccaaaccg
      781 ttcgttggcg tgctgagcgc aggtattaac gccgccagtc cgaacaaaga gctggcaaaa
      841 gagttcctcg aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa
      901 ccgctgggtg ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt
      961 gccgccacca tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc
     1021 gctttctggt atgccgtgcg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc
     1081 gatgaagccc tgaaagacgc gcagactgcc gcaggcaccg attacgatat cccaacgacc
     1141 ctggaagttc tgttccaggg gccctccggt atgagcaagg aaagacccaa gaggaatatc
     1201 attcagaaga aatacgatga cagtgatggg attccgtggt cagaagaacg ggtggtacgt
     1261 aaagtccttt atttgtctct gaaggagttc aagaactccc agaagaggca gcatgcggaa
     1321 ggcattgctg ggagcctgaa aactgtgaat gggctccttg gtaatgacca gtctaaggga
     1381 ttaggaccag catcagaaca gtcagagaat gaaaaggacg atgcatccca agtgtcctcc
     1441 actagcaacg atgttagttc ttcagatttt gaagaagggc cgtcgaggaa aaggcccagg
     1501 ctgcaagcac aaaggaagtt tgctcagtct cagccgaata gtcccagcac aactccagta
     1561 aagatagtgg agccattgct accccctcca gctactcaga tatcagacct ctctaaaagg
     1621 aagcctaaga cagaagattt tcttaccttt ctctgccttc gaggttctcc tgcgctgccc
     1681 aacagcatgg tgtattttgg aagctctcag gatgaggagg aagtcgagga ggaagatgat
     1741 gagacagaag acgtcaaaac agccaccaac aatgcttcat cttcatgcca gtcgaccccc
     1801 aggaaaggaa aaacccacaa acatgttcac aacgggcatg ttttcaatgg ttccagcagg
     1861 tcaacacggg agaaggaacc tgttcaaaaa cacaaaagca aagaggccac tcccgcaaag
     1921 gagaagcaca gcgatcaccg ggctgacagc cgccgggagc aggcttcagc taaccacccc
     1981 gcagcggccc cctccacggg ttcctcggcc aaggggcttg ctgccaccca tcaccacccc
     2041 cctctgcatc ggtcggctca ggacttacgg aaacaggttt ctaaggtaaa cggagtcact
     2101 cgaatgtcat ctctgggtgc aggtgtaacc agtgccaaaa agatgcgcga ggtcagacct
     2161 tcaccatcca aaactgtgaa gtacactgcc acggtgacga agggggctgt cacatacacc
     2221 aaagccaaga gagaactggt caaggacacc aaacccaatc accacaagcc cagttccgct
     2281 gtcaaccaca caatctcagg gaaaactgaa agtagcaatg caaaaacccg caaacaggtg
     2341 ctatccctcg ggggggcgtc caagtccact gggcccgccg tcaatggcct caaggtcagt
     2401 ggcaggttga acccaaagtc atgcactaag gaggtggggg ggcggcagct gcgggagggc
     2461 ctgcagctgc gggaggggct gcggaactcc aagaggagac tggaagaggc acaccaggcg
     2521 gagaagccgc agtcgccccc caagaagatg aaaggggcgg ctggccccgc cgaaggccct
     2581 ggcaagaagg ccccggccga gagaggtctg ctgaacggac acgtgaagaa ggaagtgccg
     2641 gagcgcagtc tggagaggaa tcggccgaag cgggccacgg ccgggaagag cacgccaggc
     2701 agacaagcac atggcaaggc ggacagcgcc tcctgtgaaa atcgttctac ctcgcaaccg
     2761 gagtccgtgc acaagccgca ggactcgggc aaggccgaga agggcggcgg caaggccggg
     2821 tgggcggcca tggacgagat ccccgtcctc aggccctccg ccaaggagtt ccacgatccg
     2881 ctcatctaca tcgagtcggt ccgcgctcag gtggagaagt tcgggatgtg cagggtgatc
     2941 ccccctccgg actggcggcc cgagtgcaag ctcaacgatg agatgcggtt tgtcacgcag
     3001 attcagcaca tccacaagct gggccggcgc tggggcccca acgtgcagcg gctggcctgc
     3061 atcaagaagc acctcaaatc tcagggcatc accatggacg agctcccgct catagggggc
     3121 tgtgagctcg acctggcctg ctttttccgg ctgattaatg agatgggcgg catgcagcaa
     3181 gtgactgacc tcaaaaaatg gaacaaacta gcagacatgc tgcgcatccc cagaactgcc
     3241 caggaccggc tggccaagct gcaggaggcc tactgccagt acctactctc ctacgactcc
     3301 ctgtccccag aggagcaccg gcggctggag aaggaggtgc tgatggagaa ggagatcctg
     3361 gagaagcgca aggggccgct ggaaggccac acagagaacg accaccacaa gttccaccct
     3421 ctgccccgct tcgagcccaa gaatgggctc atccacggcg tggcccccag gaacggcttc
     3481 cgcagcaagc tcaaggaggt gggccaggcc cagttgaaga ctggccggcg gcgactcttc
     3541 gctcaggaaa aagaagtggt caaggaagag gaggaggaca aaggcgtcct caatgacttc
     3601 cacaagtgca tctataaggg aaggtctgtt tctctaacaa ctttttatcg aacagcgagg
     3661 aatatcatga gcatgtgttt cagcaaggag cctgccccag ccgaaatcga gcaagagtac
     3721 tggaggctag tggaagagaa ggactgccac gtggcagtgc actgcggcaa ggtggacacc
     3781 aacactcacg gcagtggatt cccagtagga aaatcagaac ccttttcgag gcatggatgg
     3841 aacctcaccg tcctccccaa taacacaggg tccatcctgc gtcacctcgg tgctgtgcct
     3901 ggagtgacta ttccctggct aaatattggc atggtctttt ctacctcatg ctggtcacgc
     3961 gaccaaaatc accttccata cattgactac ttacacactg gtgctgactg catttggtat
     4021 tgcattcctg ctgaggagga gaacaagctg gaagatgtgg tccacaccct gctgcaagcc
     4081 aatggcaccc cagggctgca gatgctggaa agcaacgtca tgatctcccc ggaggtgctg
     4141 tgcaaagagg ggatcaaggt gcacaggacc gtgcagcaga gtggccagtt tgtcgtctgc
     4201 ttcccgggat cgtttgtgtc caaagtgtgc tgtgggtaca gcgtgtctga aaccgtgcac
     4261 tttgctacca cccagtggac aagtatgggc tttgagacag ccaaggaaat gaagcgtcgc
     4321 catatagcta agccattctc catggagaag ttactctacc agattgcaca agcagaagca
     4381 aaaaaagaaa acggtcccac tctcagtacc atctcagccc tcctggatga gctcagggat
     4441 acagagctgc ggcagcgcag gcagctgttc gaggctggcc tccactcctc cgcacgctat
     4501 ggcagccacg atggcagcag cacggtggcg gacgggaaga aaaagcctcg aaagtggctg
     4561 cagttggaga catcagagag gaggtgtcag atatgccagc acctgtgcta cctgtccatg
     4621 gtggtacaag agaacgaaaa cgtcgtgttc tgtctggagt gtgctctgcg ccacgtggag
     4681 aaacagaagt cctgccgagg gctgaagttg atgtaccgct acgatgagga acagattatc
     4741 agtctggtca atcagatatg cggcaaagtg tctggtaaaa acggcagcat tgagaactgt
     4801 ctcagtaaac ccacaccaaa aagaggtccc cgcaagagag cgacagtgga cgtgcccccc
     4861 tcccgtctgt cagcctccag ttcatccaaa agtgcttcga gctcatcagg atcctaa
//
