LOCUS       sequence-48862-e         493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 493)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS8
FEATURES             Location/Qualifiers
     source          1..493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc tccagagatt atcgacagaa gaagctacga ggtgggcaga ttcctttgat
      121 gtgcttctct ctcataagta tggggtggct gcattccgtg ccttcttgaa gacggagttc
      181 agtgaggaga acctggaatt ctggttggcc tgtgaggagt tcaagaagac caggtccact
      241 gcaaaactgg tctctaaggc ccataggatc tttgaggagt ttgtggatgt gcaggctcca
      301 cgggaggtaa acattgactt ccagacccga gaagccacga ggaagaacct gcaggagcca
      361 tccctgactt gctttgacca agcccaagga aaagtacaca gcctcatgga gaaagactct
      421 taccccaggt tcctgaggtc caaaatgtac ttagatctgc tgtcctgaca gtaaaggtgg
      481 atacggatcc gaa
//
