LOCUS       sequence-48863-e        1123 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1123)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SYN3
FEATURES             Location/Qualifiers
     source          1..1123
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg gactgatgga gcctccaggt ccctccacgc ccattgttca aagacccagg
      121 atcctgttgg tgatcgatga tgcccataca gactggtcga agtatttcca tgggaagaag
      181 gtgaatggag agattgagat ccgagtggag caggctgaat tctcagagtt gaacctagct
      241 gcctatgtga ccgggggctg catggtggac atgcaggtcg tgagaaatgg gaccaaagtg
      301 gtgagcagat ccttcaagcc agacttcatc ctggtccgcc agcatgccta cagcatggcc
      361 ctgggggaag actaccgcag cctggtcatc ggcctgcagt atggagggct gcctgctgtc
      421 aactctctct actccgtcta caacttctgc agcaagccct gggtgttctc tcagctcatt
      481 aagatcttcc attccctggg tcctgagaag ttcccgcttg tggagcaaac atttttcccc
      541 aaccataagc caatggtcac agccccacac ttcccggtgg tagtcaagct gggacatgcc
      601 cacgctggaa tgggaaagat caaagtggaa aaccagcttg acttccagga catcaccagc
      661 gtggtcgcca tggccaaaac ctacgccacc accgaggcct tcatcgactc caagtacgac
      721 atccgcatcc agaaaattgg atccaactac aaggcttaca tgagaacctc catctctggg
      781 aactggaagg ccaacacagg ctctgccatg ctggagcagg tggccatgac agagaggtac
      841 aggctgtggg tggacagctg ctcggaaatg tttggcggcc tggacatctg tgccgtcaag
      901 gctgtccaca gcaaggatgg cagagattac atcatcgagg taatggacag ctcaatgccg
      961 ctgattggag agcatgtgga agaggacaga cagctgatgg ccgaccttgt tgtctccaaa
     1021 atgagccagc tcccgatgcc aggaggcaca gcgccctccc ccctcagacc ttgggctcca
     1081 cagattaaat cagcgtgaca gtaaaggtgg atacggatcc gaa
//
