LOCUS       sequence-488635-        9735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHis-par2-UbHs/Ubc5Sc/ Uba1Ta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9735)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 9735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pGEN6-Ubc5 Sc
FEATURES             Location/Qualifiers
     source          1..9735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(689..708)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             700..1515
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             700..1515
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(773..792)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2012..2600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2501..2520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2754..2771
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2786..2925
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2851..2870)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3011..3033
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3027..3218)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(4027..5109)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5071..5090
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(5110..5187)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     5393..5412
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     5496..5515
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5496..5514
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5515..5539
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5554..5576
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5596..5613
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5635..5655
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5668..5895
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             8690..8701
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             9579..9596
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9649..9667)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9663..9710
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
      481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      541 ccgctcatga gacaataacc cgatccgtcg acctgcaggg ggggggggga aagccacgtt
      601 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
      661 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
      721 acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
      781 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc
      841 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
      901 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
      961 atccgtactc ctgatgatgc atggttactc accactgcga tccccgggaa aacagcattc
     1021 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     1081 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     1141 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     1201 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca
     1261 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     1321 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     1381 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     1441 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     1501 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     1561 acttgacggg acggcggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga
     1621 tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca
     1681 actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg
     1741 atggggcgat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc
     1801 gccccccccc ccctgcaggt cgacggatca agcattggta actgtcagac caagtttact
     1861 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1921 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1981 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2041 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2101 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2161 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2221 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2281 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2341 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2401 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2461 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2521 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2581 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2641 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2701 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2761 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     2821 gtatttcaca ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     2881 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     2941 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     3001 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     3061 aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt
     3121 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag
     3181 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga
     3241 tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt
     3301 actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg
     3361 atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat
     3421 gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg
     3481 cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat
     3541 gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc
     3601 ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac
     3661 aggagcacga tcatgcgcac ccgtggggcc gccatgccgg cgataatggc ctgcttctcg
     3721 ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg
     3781 aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa
     3841 atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata
     3901 agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
     3961 ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg
     4021 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     4081 ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac
     4141 cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa
     4201 gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg
     4261 gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac
     4321 gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac
     4381 cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga
     4441 catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata
     4501 tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag
     4561 cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc
     4621 atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg
     4681 aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat
     4741 gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac
     4801 gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt
     4861 aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat
     4921 cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc
     4981 cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac
     5041 cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac
     5101 tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg
     5161 aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct
     5221 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
     5281 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
     5341 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
     5401 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
     5461 cggcgtagag gatcgagatc tcgatcccgc gaaattaata cgactcacta taggggaatt
     5521 gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata
     5581 catatgtcgt actaccatca ccatcaccat cacgattacg atatcccaac gaccgaaaac
     5641 ctgtattttc agggcgccat gggatccatg cagatcttcg tgaagactct gactggtaag
     5701 accatcaccc tcgaggtgga gcccagtgac accatcgaga atgtcaaggc aaagatccaa
     5761 gataaggaag gcattcctcc tgatcagcag aggttgatct ttgccggaaa acagctggaa
     5821 gatggtcgta ccctgtctga ctacaacatc cagaaagagt ccaccttgca cctggtactc
     5881 cgtctcagag gtgggtgaga attctaagga aatccattat gtcttcctcc aagcgtattg
     5941 ccaaggaatt aagtgattta gggagagatc ctcctgcttc atgttcagca ggacctgtag
     6001 gggatgacct gtatcattgg caagcctcta ttatgggtcc ttcagactca ccctacgctg
     6061 gtggcgtttt ctttttgtct attcactttc caactgatta tccattcaag ccaccgaagg
     6121 taaactttac gaccaaaatt tatcatccga atattaattc gagtggtaat atctgccttg
     6181 atattttaaa ggaccagtgg tcaccggcgc taaccctttc aaaagttttg ttgtctattt
     6241 gctctctttt aacagatgct aatcccgacg atcctttggt ccctgaaatt gctcaaatct
     6301 acaagacaga taaggctaag tatgaagcca ccgctaagga gtggactaaa aaatatgctg
     6361 tttgaggcgc gccacaattc cgacaggaaa cagctatgct tccccggaag cgggagatcg
     6421 tcgccggcga ggtcgaggac ttgcagaaga agacccgcgc cggggagggg gaggtcacta
     6481 gggaggaagg agacgcagcc atggcaggga gggggaacga gatcgacgag gacctgcaca
     6541 gccgccagct cgccgtctat gggcgcgaga ccatgaagcg cctcttcggc tccaacgtcc
     6601 tcgtctctgg actccagggt ctcggtgctg agatcgcaaa gaaccttgtc cttgcgggtg
     6661 tcaagtctgt aaccttgcat gatgatggta acgtggagct gtgggactta tcaagcaact
     6721 tcttcctctc ggagaatgat gttgggcaaa accgtgctca agcttgtgta cagaagctac
     6781 aagagctgaa caatgctgtc ctcgtctctg ccttaaccgg tgatttgacc aaggagcacc
     6841 tttctaaatt ccaggccgtt gtcttcactg atatcagctt agataaagca attgaatttg
     6901 atgattactg ccacagccaa cagccaccca ttgcgttcat caagtctgaa gttcgtggtc
     6961 tttttggcag tgttttttgt gactttggtc ctgaatttac tgttttggat gtggatggag
     7021 aggaaccaca tacaggaatt gttgcatcaa tcagcaatga caatccagca cttgtatctt
     7081 gtgtggacga tgagcgtctg gagttccagg atggtgatct agttgttttc tctgaagtcc
     7141 atggaatgac tgagctgaat gatggaaaac caagaaaagt taagaatgca aggccttact
     7201 ctttcttctt ggaagaagac acttcctcat ttggcgcata tgttagaggt ggtattgtca
     7261 cacaggtcaa gccaccgaag gttattaaat tcaaaccctt gaaagaggcc atgtcagagc
     7321 cgggagaatt tctcatgagt gatttctcca aatttgagcg gccacctctt ctgcatttgg
     7381 cattccaagc tttggataag tttaggactg agttgagcag attccctgtt gctgggtcca
     7441 ctgatgatgt gcaaagggtg atagagtatg ctattagcat taatgatact ctgggagata
     7501 ggaaacttga agaaattgac aaaaagctcc tgcatcattt tgccagtggt tccagggctg
     7561 ttctgaatcc tatggctgcg atgtttggtg gtattgtggg tcaggaggta gtgaaagctt
     7621 gctcagggaa atttcatcca ctttatcagt tcttctattt tgattctgtc gagtctctcc
     7681 ctgttgaccc cttggagcct ggtgatttga agccgaagaa cagtagatat gatgctcaaa
     7741 tcagcgtatt tggatctaag cttcaaaaca aactggagga agcaaaaatc tttatggtcg
     7801 gttctggggc acttggatgt gagttcttga agaacctggc actaatgggt atttcttgca
     7861 gccagaatgg aaatctgact ctgacagatg atgatgtcat tgaaaagagt aatctgagcc
     7921 gccaatttct tttccgtgac tggaacattg ggcaacctaa gtccacagtt gctgctacag
     7981 ctgctatggt aattaatcct aaacttcatg tcgaggccct tcagaacaga gcaagtcctg
     8041 agactgaaaa tgtgtttaat gatgccttct gggagaacct tgatgctgtt gtcaatgccc
     8101 ttgacaatgt tactgcaaga atgtacatag actccagatg tgtatatttc cagaagccac
     8161 ttttggaatc tgggactctg ggtgctaaat gcaatacaca gatggtcatc cctcacctaa
     8221 cagagaacta tggggcttca agggatccac ctgaaaagca ggcacctatg tgcactgtac
     8281 attcatttcc tcataacatt gatcattgcc taacctgggc taggtctgag tttgagggtt
     8341 tacttgagaa gactcccact gaagtaaatg ctttcctgtc aaatcctact acatacataa
     8401 gtgcagcaag aactgctggt gatgcacagg ctagagatca gcttgaacgt gttattgagt
     8461 gtcttgacag agacaagtgc gagacgttcc aggattctat tacctgggcc cggcttaagt
     8521 ttgaggatta cttctccaac cgtgtgaagc agctgacgtt cactttccca gaagactcga
     8581 tgaccagctc tggtgctcct ttctggtctg ctccaaagcg gttcccacga cctgtggagt
     8641 tctcgtccag tgatcagagt cagcttagct ttattttggc tgctgcaata ttgagggcag
     8701 agacttttgg aatacccata cctgagtggg ccaaaacccc aaacaagctg gctgctgaag
     8761 ctgtcgacaa ggtgattgtc ccggattttc aaccaaagca gggggttaag atagttacag
     8821 atgagaaggc cactagtctg tcctctgcat ctgttgacga cgctgctgtc attgaagagc
     8881 ttattgccaa gttagaagaa gtttccaaaa cactgccatc agggttccac atgaacccaa
     8941 tacagtttga gaaggatgat gacacaaact tccatatgga tgtgatagct ggttttgcca
     9001 acatgagggc aaggaactac agcattcctg aagtggacaa gctcaaggcc aagtttatag
     9061 ccggcaggat catcccagct atcgccacct ccaccgcgat ggccactggc ctcgtctgcc
     9121 ttgagcttta caaagccctt gctggtggac acaaggttga agactaccgc aacacgtttg
     9181 caaacctcgc tatccctctc ttctccattg ctgaaccagt tccacccaag accatcaagc
     9241 accaggagtt gtcgtggacg gtctgggacc ggtggactgt gacgggcaac atcacgctga
     9301 gggagctcct ggagtggctc aaggagaagg gcctgaacgc ttacagcata tcctgtggca
     9361 cctcgctgct gtacaactcc atgttcccca ggcacaagga acggcttgac aggaaggtag
     9421 ttgatgttgc cagggaggtg gccaagatgg aggtgccctc ttaccggcgt catctcgacg
     9481 tcgtggtggc gtgtgaggat gacgacgaca atgatgtcga catcccactt gtgtcggtct
     9541 acttccgctg attcgaatct agagcctgca gtctcgagca ccaccaccac caccactgag
     9601 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     9661 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
     9721 gaactatatc cggat
//
