LOCUS       sequence-488686-       12348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express 3xFlag C-terminal tagged wild-type mouse ADCY3 transiently 
            via transfection or stably via lentivirus-mediated infection in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12348)
  AUTHORS   Xun Y, Jiang Y, Xu B, Tang M, Ludwig S, Nakamura K, Mukhopadhyay S, 
            Liu C, Beutler B, Zhang Z
  TITLE     GPR45 modulates Galpha(s) at primary cilia of the paraventricular 
            hypothalamus to control food intake.
  JOURNAL   Science. 2025 Jun 5;388(6751):eadp3989. doi: 
            10.1126/science.adp3989. Epub 2025 Jun 5.
  PUBMED    40472089
REFERENCE   2  (bases 1 to 12348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-06-5"; volume: "388(6751):eadp3989. doi"; pages: " 
            10.1126/science.adp3989. Epub 2025 Jun 5"
COMMENT     Sequence Label: pBOB-Adcy3-3xFlag
FEATURES             Location/Qualifiers
     source          1..12348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6592..6657
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          6749..6978
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     6961..6980
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    7155..7743
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7208..7228)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7626..7637)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8150..8330
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8356..8373)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8362..8586
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8632..9060
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8719..8738)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8929..8950
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9069..9089)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9074..9403
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9254..9389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9316..9335
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9451..9498
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9517..9891
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10021..10142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10058..10077)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10112..10131
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10191..10207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10191..10207)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10204..10226)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10215..10231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10239..10245,10246..10263,10264..10269))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10284..10305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10422..10439)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10593..11181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10673..10692)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11352..12143,12144..12212))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11975..11994
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12213..12317)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagaattcgg atccgccacc atgccgagga accagggctt ctcggatccc
     3181 gagtactcgg cagagtactc agctgaatac tcggtcagcc tgccctctga ccccgaccgc
     3241 ggggtcggcc ggacccatga aatttctgtg cggaactcgg ggtcctgcct gtgcctgcct
     3301 cgctttatgc ggctgacctt cgtgcctgag tccttggaga acctctacca gacctacttc
     3361 aaaaggcagc gccatgagac cctgctggtg ctggtggtct ttgcggccct ctttgactgc
     3421 tacgtggtag tgatgtgcgc ggtggtcttc tccagcgaca agctggcgcc cctcatggtg
     3481 gcaggcttcg gtctggtgtt ggacatcatc cttttcgtgc tctgtaaaaa ggggctgctc
     3541 ccggaccgag tgagccgcaa agtggtaccc tacctgctgt ggctgctcat ctcggcccag
     3601 atcttctcct acctgggcct gaacttttca cgggcccacg cagccagtga cactgtgggt
     3661 tggcaggctt tctttgtctt ctccttcttc ataacgctgc ccctcagcct cagccccatc
     3721 gtgatcatct ctgtggtctc ctgtgttgtg cacacgcttg tgttgggggt cactgtggcc
     3781 cagcagcagc aggatgagct ggaagggatg cagctgctga gggagatcct ggccaacgtc
     3841 ttcctctacc tgtgtgctat catcgtgggc atcatgtcct actacatggc agaccgcaag
     3901 caccgcaagg ccttcctgga ggcccgccag tcactggagg tgaagatgaa tctggaggag
     3961 cagagccagc agcaggaaaa ccttatgctt tccatcctgc ccaagcacgt ggctgacgag
     4021 atgctgaagg acatgaagaa ggacgagagc cagaaggacc agcagcagtt caataccatg
     4081 tacatgtacc ggcacgagaa tgtcagcatc ctgtttgcag atattgtggg ctttacccag
     4141 ctgtcctctg cttgcagtgc ccaggagctc gtgaagttac tcaacgagct cttcgcccgc
     4201 tttgacaagc tggcagccaa ataccaccag ctgaggatca agatcctagg cgactgttac
     4261 tactgcatct gcggcttgcc tgactaccgg gaggaccatg ccgtgtgctc catccttatg
     4321 gggcttgcca tggtagaggc catctcgtac gtgcgggaga agaccaagac tggagtggac
     4381 atgcgtgtgg gagtgcacac aggcactgtc ctaggcggtg tcctgggcca gaagcgctgg
     4441 cagtatgatg tatggtctac tgatgtcacc gtggcaaaca agatggaggc tggtggcatc
     4501 ccagggcgcg tgcacatttc ccagagcacc atggactgcc tgaaggggga gtttgatgtg
     4561 gagcctggtg atgggggcag tcgctgcgac tacctagatg agaagggcat cgaaacctac
     4621 ctcatcattg cctccaagcc agaggtgaag aaaacagccc aaaatggcct caacggctcg
     4681 gccgtgccaa acggagcgcc ggcatcctcc aaacccagct cccctgccct catcgagacc
     4741 aaggagccca atggaagtgc ccatgccagc ggctccacat cagaggaggc tgaagaacag
     4801 gaggcccagg ctgacaaccc ctcgttcccc aacccccgcc gcaggctgcg cctccaggac
     4861 ctggcggacc gtgtggtgga cgcctctgag gatgagcacg aactgaacca gctgcttaat
     4921 gaggccctgc tggagcggga gtccgcccag gtggtaaaga agagaaacac attcctcctg
     4981 accatgaggt tcatggaccc agagatggaa acacgctact cggtggagaa ggagaagcag
     5041 agcggggctg ccttcagctg ctcctgtgtg gtcctcttct gcacggccat ggtggagata
     5101 ctcattgacc cctggctgat gacaaactat gtgacctttg tggttgggga ggttctgctc
     5161 ctgatcctga ccatctgctc gatggctgcc atctttccca ggtcatttcc caagaagctt
     5221 gtggccttct catcttggat tgaccggacc cgctgggcaa ggaacacctg ggccatgtta
     5281 gccatcttca ttctggttat ggccaatgtt gtggacatgc tcagctgtct ccagtactac
     5341 atgggacctt acaacatgac agctgggatg gagctggacg gcggctgcat ggagaacccc
     5401 aagtactaca actacgtagc tgtgctgtcg ctcatcgcca ccatcatgct ggtgcaggtc
     5461 agccacatgg tgaagctgac gctcatgctg ctcgtcacag gcgccgtgac tgccctcaac
     5521 ctgtacgcct ggtgtcctgt ctttgatgaa tacgaccaca agcgctttca ggaaaaggac
     5581 tctcctatgg tggccttaga gaagatgcag gtacttgcca cccctgggct caatggcact
     5641 gacagcaggc tgcccctggt gccttccaag tactccatga ctgtgatgat gttcgtcatg
     5701 atgctgagct tttactactt ctcgcgccac gtggaaaaac tggcccgaac actgttcttg
     5761 tggaagattg aggtccatga ccagaaagaa cgtgtctacg agatgcgccg atggaacgag
     5821 gccttggtca ccaacatgtt gcctgagcat gttgcacgcc attttctggg gtccaagaag
     5881 agagatgagg agctgtacag ccagtcttat gacgagattg gagtcatgtt tgcctccttg
     5941 cccaactttg ctgacttcta cactgaggag agcatcaaca acggcggcat cgagtgtcta
     6001 cgcttcctca atgagatcat ctctgatttt gactctctcc tggacaatcc caaattccgg
     6061 gtcatcacca agatcaaaac tattggcagc acctatatgg cagcttcagg agtcacacca
     6121 gatgtcaaca ccaatggctt tacaagctcc agcaaggagg agaagtcaga caaggagcgc
     6181 tggcagcacc tggctgacct ggctgacttt gcattagcca tgaaggacac gctcacaaac
     6241 atcaacaacc agtcattcaa caacttcatg ttgcgcatag gcatgaacaa aggaggggtt
     6301 ctggctggcg tcattggagc ccggaaacca cactatgaca tctggggcaa tacggtcaat
     6361 gtggccagca ggatggaatc cacaggagtc atgggcaaca tccaggtggt agaagagacg
     6421 caggtcatcc ttcgagagta cggcttccgc tttgtgaggc gaggtcccat ctttgtgaaa
     6481 ggcaaagggg agcttctgac ctttttcttg aaggggcggg acaggccagc tgccttcccc
     6541 aatggctcct ctgttacact gccccaccaa gtggtggaca accccctcga ggactacaaa
     6601 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtag
     6661 tgatcgagcc atggaagctt gatatctaac tgactgaacc ggtggtaccg aggaattaat
     6721 tcgctgtctg cgagggccag ctgttggggt gagtactccc tctcaaaagc gggcatgact
     6781 tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt tgatattcac ctggcccgcg
     6841 gtgatgcctt tgagggtggc cgcgtccatc tggtcagaaa agacaatctt tttgttgtca
     6901 agcttgaggt gtggcaggct tgagatctgg ccatacactt gagtgacaat gacatccact
     6961 ttgcctttct ctccacaggt gtccactccc aggtccaact gcaggtcgag catgcatcta
     7021 gggcggccaa ttccgccgat cctggaaaaa catggagcaa tcacaagtag caatacagca
     7081 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     7141 gtcacacctc agacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     7201 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     7261 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     7321 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7381 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     7441 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     7501 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     7561 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7621 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7681 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7741 cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct
     7801 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     7861 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     7921 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     7981 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     8041 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     8101 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     8161 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8221 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8281 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggccgcttt
     8341 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     8401 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     8461 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     8521 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     8581 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     8641 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     8701 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     8761 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     8821 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     8881 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     8941 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     9001 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     9061 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     9121 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     9181 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     9241 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     9301 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     9361 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     9421 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     9481 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     9541 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     9601 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     9661 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     9721 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     9781 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     9841 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     9901 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     9961 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
    10021 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    10081 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    10141 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
    10201 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    10261 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    10321 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    10381 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    10441 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    10501 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    10561 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    10621 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    10681 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    10741 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    10801 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    10861 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    10921 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    10981 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    11041 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    11101 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    11161 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    11221 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    11281 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    11341 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    11401 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    11461 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    11521 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    11581 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    11641 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    11701 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    11761 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    11821 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    11881 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    11941 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    12001 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    12061 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    12121 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    12181 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    12241 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    12301 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
