LOCUS       sequence-488700-        9238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FOXO1 - Insulin (PI3K/AKT) gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE8
FEATURES             Location/Qualifiers
     source          1..9238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1919
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2068..2099
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2126..2135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2132..3781
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3785..3904
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    3996..4584
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4049..4069)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4467..4478)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4599..4915
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4766..4901
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4966..4968,4969..4971,4972..5685)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5011..5032)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5272..5291)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5619..5640
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5757..5990
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6062..6183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6099..6118)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6153..6172
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6223..6358
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6378..6397)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6379..6397)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6407..6424)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6407..6423)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6416..6438)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6565..7020
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6652..6671)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6862..6883
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7046..7150
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7151..7219,7220..8011)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7369..7388)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8182..8770
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8671..8690
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8924..8941
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9058..9079
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9094..9099,9100..9117,9118..9124)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9132..9148
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9137..9159
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9156..9172
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9156..9172
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9191..9211
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9193..9211
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta
     1921 tcgatcgcac cagcgtgtgg atctcgaaag taaacaacta tgtaaacaac tataagtaaa
     1981 caactatgta aacaactata agtaaacaac tatgtaaaca agatcgatcc gagaacagat
     2041 ctggcctcgg cggccaagct tagacactag agggtatata atggaagctc gacttccagc
     2101 ttggcaatcc ggtactgttg gtaaagccac catggaagat gccaaaaaca ttaagaaggg
     2161 cccagcgcca ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat
     2221 gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga
     2281 cattacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta
     2341 tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat
     2401 gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa
     2461 cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa
     2521 gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat
     2581 catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc
     2641 ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa
     2701 aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct
     2761 accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca
     2821 gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt
     2881 caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga
     2941 ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac
     3001 actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca
     3061 cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg
     3121 cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct
     3181 gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga
     3241 ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct
     3301 gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc
     3361 tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga
     3421 gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc
     3481 cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc
     3541 cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg
     3601 taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa
     3661 gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt
     3721 ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt
     3781 gaattctcac ggcttccctc ccgaggtgga ggagcaggcc gccggcaccc tgcccatgag
     3841 ctgcgcccag gagagcggca tggatagaca ccctgctgct tgcgccagcg ccaggatcaa
     3901 cgtctaaggc cgcgactcta gaacgcgtca ctgcggctac cccgtggagc gagatgacta
     3961 cagacatcag cgcgtaccgg ttagtaatga tcgacaatca acctctggat tacaaaattt
     4021 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4081 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4141 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4201 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4261 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4321 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4381 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     4441 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4501 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4561 tctccctttg ggccgcctcc ccgcctggcg atggtaccgg tgtggaaagt ccccaggctc
     4621 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     4681 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4741 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4801 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     4861 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     4921 cccgggagct tgtatatcca ttttcggatc tgatcagcac ctagcatggt gagcaagggc
     4981 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     5041 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     5101 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
     5161 acctacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     5221 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     5281 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     5341 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     5401 tacaacagcc acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac
     5461 ttcaagaccc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     5521 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     5581 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5641 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaggtac ctttaagacc
     5701 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5761 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     5821 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5881 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5941 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     6001 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     6061 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6121 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6181 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     6241 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6301 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6361 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     6421 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     6481 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     6541 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6601 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6661 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6721 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6781 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6841 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6901 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6961 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     7021 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7081 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7141 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7201 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7261 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7321 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7381 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7441 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7501 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7561 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7621 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7681 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7741 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7801 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7861 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7921 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7981 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8041 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8101 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8161 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8221 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8281 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8341 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8401 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8461 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8521 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8581 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8641 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8701 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8761 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8821 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8881 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8941 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9001 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9061 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     9121 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     9181 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaa
//
