LOCUS       sequence-488733-        9282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of mRuby2-HRAS(G12V).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9282)
  AUTHORS   Lende-Dorn BA, Atkinson JC, Bae Y, Galloway KE
  TITLE     Chemogenetic tuning reveals optimal MAPK signaling for cell-fate 
            programming
  JOURNAL   bioRxiv 2024.12.19.629350
REFERENCE   2  (bases 1 to 9282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.12.19.629350"
COMMENT     Sequence Label: pLentiX1-CAG-mRuby2-HRAS(G12V)
FEATURES             Location/Qualifiers
     source          1..9282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        42..146
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(147..215,216..1007)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(365..384)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1178..1766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1667..1686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1920..1937
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2054..2075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2090..2095,2096..2113,2114..2120)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2128..2144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2133..2155
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2152..2168
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2152..2168
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2187..2207
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2189..2207
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2235..2461
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2462..2642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2689..2814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3307..3540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3725..3769
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4036..4152
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4203..4227
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        4332..4635
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4637..4913
                     /label=chicken beta-actin promoter
     intron          4914..5930
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5854..5876
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5938..5957
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      5983..5992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5989..6699
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             join(6730..6762,6763..6765,6766..7299)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     misc_feature    7310..7898
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7363..7383)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7781..7792)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7912..7936)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             8035..8268
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8340..8461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8377..8396)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8431..8450
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8501..8636
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8656..8675)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8657..8675)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8685..8702)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8685..8701)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8694..8716)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8843..9271
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8930..8949)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9140..9161
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
       61 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      121 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      181 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      241 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      301 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      361 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      421 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
      481 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
      541 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
      601 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
      661 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
      721 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
      781 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
      841 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
      901 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
      961 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1021 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1081 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1141 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1201 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1261 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1321 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1381 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1441 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1501 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1561 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1621 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1681 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1741 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1801 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1861 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     1921 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     1981 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     2041 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     2101 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     2161 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     2221 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     2281 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     2341 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     2401 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     2461 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     2521 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     2581 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     2641 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     2701 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     2761 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     2821 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     2881 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     2941 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     3001 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     3061 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     3121 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     3181 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     3241 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     3301 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     3361 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     3421 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     3481 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     3541 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     3601 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     3661 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     3721 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     3781 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     3841 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     3901 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     3961 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
     4021 acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     4081 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     4141 attcaaaatt ttatcgtcca gtgtggtggc tactcatagc aggtgccact agtggagttc
     4201 cgacaagttt gtacaaaaaa gcaggctcca ccatgggaac caattcagtc gactggatcc
     4261 ggtactacta ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     4321 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     4381 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     4441 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     4501 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     4561 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     4621 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     4681 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     4741 gcgggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg
     4801 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
     4861 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     4921 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     4981 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     5041 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
     5101 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
     5161 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
     5221 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     5281 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     5341 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     5401 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     5461 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     5521 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc
     5581 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     5641 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     5701 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     5761 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     5821 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     5881 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     5941 acgtgctggt tattgtgctg tctcatcatt ttggcaaaaa tggccaccat ggtgtctaag
     6001 ggcgaagagc tgatcaagga aaatatgcgt atgaaggtgg tcatggaagg ttcggtcaac
     6061 ggccaccaat tcaaatgcac aggtgaagga gaaggcaatc cgtacatggg aactcaaacc
     6121 atgaggatca aagtcatcga gggaggaccc ctgccatttg cctttgacat tcttgccacg
     6181 tcgttcatgt atggcagccg tacttttatc aagtacccga aaggcattcc tgatttcttt
     6241 aaacagtcct ttcctgaggg ttttacttgg gaaagagtta cgagatacga agatggtgga
     6301 gtcgtcaccg tcatgcagga caccagcctt gaggatggct gtctcgttta ccacgtccaa
     6361 gtcagagggg taaactttcc ctccaatggt cccgtgatgc agaagaagac caagggttgg
     6421 gagcctaata cagagatgat gtatccagca gatggtggtc tgaggggata cactcatatg
     6481 gcactgaaag ttgatggtgg tggccatctg tcttgctctt tcgtaacaac ttacaggtca
     6541 aaaaagaccg tcgggaacat caagatgccc ggtatccatg ccgttgatca ccgcctggaa
     6601 aggttagagg aaagtgacaa tgaaatgttc gtagtacaac gcgaacacgc agttgccaag
     6661 ttcgccgggc ttggtggtgg gatggacgag ctgtacaagg gtggtggggg aagcggagga
     6721 ggaggttcga tgaccgaata caagctggtg gtggtgggcg ccgtcggtgt gggcaagagt
     6781 gcgctgacca tccagctgat ccagaaccat tttgtggacg aatacgaccc cactatagag
     6841 gattcctacc ggaagcaggt ggtcattgat ggggagacgt gcctgttgga catcctggat
     6901 accgccggcc aggaggagta cagcgccatg cgggaccagt acatgcgcac cggggagggc
     6961 ttcctgtgtg tgtttgccat caacaacacc aagtcttttg aggacatcca ccagtacagg
     7021 gagcagatca aacgggtgaa ggactcggat gacgtgccca tggtgctggt ggggaacaag
     7081 tgtgacctgg ctgcacgcac tgtggaatct cggcaggctc aggacctcgc ccgaagctac
     7141 ggcatcccct acatcgagac ctcggccaag acccggcagg gagtggagga tgccttctac
     7201 acgttggtgc gtgagatccg gcagcacaag ctgcggaagc tgaaccctcc tgatgagagt
     7261 ggccccggct gcatgagctg caagtgtgtg ctctcctaac aacgccgata atcaacctct
     7321 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7381 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7441 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7501 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7561 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7621 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7681 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     7741 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7801 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7861 tcagacgagt cggatctccc tttgggccgc ctccccgccg ctgatatcta gacccagctt
     7921 tcttgtacaa agtggtcacc tgctatgcgc tgatataggg cccggtacct ttaagaccaa
     7981 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     8041 ggctagctca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     8101 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8161 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8221 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     8281 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     8341 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8401 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8461 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     8521 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8581 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8641 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     8701 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     8761 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     8821 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     8881 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     8941 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     9001 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     9061 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     9121 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     9181 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     9241 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa ta
//
