LOCUS       sequence-48876-e         616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 616)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DIRAS2
FEATURES             Location/Qualifiers
     source          1..616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga gtaacgatta ccgggtggcc gtgtttgggg ctggcggtgt tggcaagagc
      121 tccctggtgt tgaggtttgt gaaaggcaca ttccgggaga gctacatccc gacggtggaa
      181 gacacctacc ggcaagtgat cagctgtgac aagagcatat gcacattgca gatcaccgac
      241 acgacgggga gccaccagtt cccggccatg cagcggctgt ccatctccaa agggcacgcc
      301 ttcatcctgg tgtactccat taccagccga cagtccttgg aggagctcaa gcccatctac
      361 gaacaaatct gcgagatcaa aggggacgtg gagagcatcc ccatcatgct ggtggggaac
      421 aagtgtgatg agagccccag ccgcgaggtg cagagcagcg aggcggaggc cttggcccgc
      481 acatggaagt gtgccttcat ggagacctca gccaagctca accataacgt gaaggagctt
      541 ttccaggagc tgctcaacct ggagaagcgc aggaccgtga gtctccagtg acagtaaagg
      601 tggatacgga tccgaa
//
