LOCUS       sequence-48877-e         661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 661)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GEM
FEATURES             Location/Qualifiers
     source          1..661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgt ccacagactc agtcatctcc tctgagtcag ggaacaccta ctaccgagtg
      121 gtgctcatag gggagcaggg ggtgggcaag tccactctgg ccaacatctt tgcaggtgtg
      181 catgacagca tggacagcga ctgcgaggtg ctgggagaag atacatatga acgaaccctg
      241 atggttgatg gggaaagtgc aacgattata ctcctggata tgtgggaaaa taagggggaa
      301 aatgaatggc tccatgacca ctgcatgcag gtcggggacg catacctgat tgtctactca
      361 atcacagacc gagcgagctt cgagaaggca tctgagctgc gaatccagct ccgcagggcc
      421 cggcagacag aggacattcc cataattttg gttggcaaca aaagtgactt agtgcggtgc
      481 cgagaagtgt ctgtatcaga agggagagcc tgtgcagtgg tgtttgactg caagttcatc
      541 gagacctctg cagctgtcca gcacaacgtg aaggagctgt ttgagggcat tgtgcgacag
      601 gtgcgccttc ggcgggacag caaggagaag aattgacagt aaaggtggat acggatccga
      661 a
//
