LOCUS       sequence-488849-       11332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To amplify the virus that expresses C-mCherry GAC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11332)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 11332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pBOBI C-mCherry GAC
FEATURES             Location/Qualifiers
     source          1..11332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4939..5646
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5084..5102)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5352..5371
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          5733..5962
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5945..5964
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    6139..6727
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6192..6212)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6610..6621)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7134..7314
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7340..7357)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7346..7570
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7616..8044
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7703..7722)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7913..7934
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8053..8073)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8058..8387
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8238..8373
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8300..8319
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8435..8482
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8501..8875
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9005..9126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9042..9061)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9096..9115
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9175..9191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9175..9191)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9188..9210)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9199..9215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9223..9229,9230..9247,9248..9253))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9268..9289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9406..9423)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9577..10165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9657..9676)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10336..11127,11128..11196))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10959..10978
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11197..11301)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagaattcgg atccatgatg cggctgcgag gctcggggat gctgcgggac
     3181 ctgctcctgc ggtcgcccgc aggagtatcg gcgactctgc ggcgggcaca gcccttggtc
     3241 accctgtgcc ggcgtccccg aggcggggga cggccggccg cgggcccggc tgccgccgcg
     3301 cgactccacc cgtggtgggg cgggggcggc tggccggcgg agcccctcgc gcggggcctg
     3361 tccagctctc cttcggagat cttgcaggag ctgggcaagg ggagcacgca tccgcagccc
     3421 ggggtgtcgc cacccgctgc cccggcggcg cccggcccca aggacggccc cggggagacg
     3481 gacgcgtttg gcaacagcga gggcaaagag ctggtggcct caggtgaaaa taaaataaaa
     3541 cagggtctgt tacctagctt ggaagatttg ctgttctata caattgctga aggacaagag
     3601 aaaatacctg ttcataaatt tattacagca ctcaaatcta caggattgcg aacgtctgat
     3661 cccaggttga aagagtgtat ggatatgtta agattaactc ttcaaacaac atcagatggt
     3721 gtcatgctag acaaagatct ttttaaaaaa tgtgttcaga gcaacattgt tttgttgaca
     3781 caagcattta gaagaaagtt tgtgattcct gactttatgt cttttacctc acacattgat
     3841 gagttatatg aaagtgctaa aaagcagtct ggaggaaagg ttgcagatta tattcctcaa
     3901 ctggccaaat tcagtcccga tttgtggggt gtgtctgttt gtacagtaga tggacagagg
     3961 cattctactg gagataccaa agttcccttc tgtcttcagt cctgtgtaaa acctttgaaa
     4021 tatgccattg ctgttaatga tcttggaact gaatatgtgc atcgatatgt tggaaaagag
     4081 ccgagtggac taagattcaa caaactattt ttgaatgaag atgataaacc acataatcct
     4141 atggtaaatg ctggagcaat tgttgtgact tcactaataa agcaaggagt aaataatgct
     4201 gaaaaatttg actatgtcat gcagtttttg aataagatgg ctggtaatga atatgttgga
     4261 ttcagtaatg caacgtttca gtctgaaaga gaaagtggag atcgaaattt tgcaatagga
     4321 tattacttaa aagaaaagaa gtgttttcca gaaggcacag acatggttgg tatattagac
     4381 ttctacttcc agctgtgctc cattgaagtg acttgtgaat cagccagtgt gatggctgcg
     4441 acactggcta atggtggttt ctgcccaatt actggtgaaa gagtactgag ccctgaagca
     4501 gttcgaaata cattgagttt gatgcattcc tgtggcatgt atgacttctc agggcagttt
     4561 gctttccatg ttggtcttcc tgcaaaatct ggagttgctg ggggcattct tttagttgtc
     4621 cccaatgtta tgggtatgat gtgctggtct cctcctctgg ataagatggg caacagtgtt
     4681 aagggaattc acttttgtca cgatcttgtt tctctgtgta atttccataa ctatgataat
     4741 ttgagacact ttgcaaaaaa acttgatcct cgaagagaag gtggtgatca aaggcattcc
     4801 tttggaccat tggactatga aagtctccaa caagaacttg ctttaaaaga gacagtatgg
     4861 aaaaaagtgt cacctgagtc aaatgaggac atctctacaa ctgtagtata tagaatggaa
     4921 agtctgggag agaaaagcat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     4981 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     5041 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     5101 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     5161 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     5221 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     5281 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     5341 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     5401 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     5461 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     5521 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     5581 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     5641 tacaagctcg agccatggaa gcttgatatc taactgactg aaccggtggt accgaggaat
     5701 taattcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     5761 gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     5821 cgcggtgatg cctttgaggg tggccgcgtc catctggtca gaaaagacaa tctttttgtt
     5881 gtcaagcttg aggtgtggca ggcttgagat ctggccatac acttgagtga caatgacatc
     5941 cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcaggt cgagcatgca
     6001 tctagggcgg ccaattccgc cgatcctgga aaaacatgga gcaatcacaa gtagcaatac
     6061 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     6121 tccagtcaca cctcagacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6181 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6241 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6301 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6361 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6421 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6481 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     6541 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6601 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6661 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6721 tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     6781 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6841 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     6901 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     6961 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     7021 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     7081 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     7141 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7201 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7261 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccg
     7321 ctttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     7381 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     7441 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     7501 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     7561 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     7621 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7681 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7741 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7801 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7861 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7921 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7981 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8041 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     8101 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     8161 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     8221 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8281 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     8341 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     8401 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     8461 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     8521 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     8581 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     8641 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     8701 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     8761 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     8821 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     8881 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8941 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     9001 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9061 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9121 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     9181 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     9241 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9301 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9361 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9421 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9481 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9541 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9601 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9661 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9721 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9781 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9841 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9901 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9961 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    10021 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10081 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10141 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10201 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10261 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10321 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10381 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10441 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10501 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10561 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10621 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10681 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10741 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10801 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10861 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10921 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10981 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    11041 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    11101 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    11161 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11221 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11281 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
