LOCUS       sequence-48886-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AK2
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      221..230
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg ctcccagcgt gccagcggca gaacccgagt atcctaaagg catccgggcc
      121 gtgctgctgg ggcctcccgg ggccggtaaa gggacccagg cacccagatt ggctgaaaac
      181 ttctgtgtct gccatttagc tactggggac atgctgaggg ccatggtggc ttctggctca
      241 gagctaggaa aaaagctgaa ggcaactatg gatgctggga aactggtgag tgatgaaatg
      301 gtagtggagc tcattgagaa gaatttggag acccccttgt gcaaaaatgg ttttcttctg
      361 gatggcttcc ctcggactgt gaggcaggca gaaatgctcg atgacctcat ggagaagagg
      421 aaagagaagc ttgattctgt gattgaattc agcatcccag actctctgct gatccgaaga
      481 atcacaggaa ggctgattca ccccaagagt ggccgttcct accacgagga gttcaaccct
      541 ccaaaagagc ccatgaaaga tgacatcacc ggggaaccct tgatccgtcg atcagatgat
      601 aatgaaaagg ccttgaaaat ccgcctgcaa gcctaccaca ctcaaaccac cccactcata
      661 gagtactaca ggaaacgggg gatccactcc gccatcgatg catcccagac ccccgatgtc
      721 gtgttcgcaa gcatcctagc agccttctcc aaagccacat gtaaagactt ggttatgttt
      781 atcttgtgac agtaaaggtg gatacggatc cgaa
//
