LOCUS       sequence-488888-       13564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a chimeric form of ALDH18A1, where the region between 
            residue 97 and 351 is replaced by a 250-residue XTEN linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13564)
  AUTHORS   Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, 
            Ting AY, Weissman JS
  TITLE     Proximity-specific ribosome profiling reveals the logic of localized
            mitochondrial translation.
  JOURNAL   Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 
            10.1016/j.cell.2025.08.002.
  PUBMED    40876456
REFERENCE   2  (bases 1 to 13564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002."
COMMENT     Sequence Label: pJH061
FEATURES             Location/Qualifiers
     source          1..13564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2610..3198
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2716..2727
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3125..3145
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3223..3825)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3309..3329)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3806..3825
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3837..4337)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3900..3918)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        4424..5602
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4655..5593
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5550..5570
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     LTR             9369..9549
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9575..9592)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9581..9805
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9851..10279
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9938..9957)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10148..10169
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10288..10308)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10293..10622
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10473..10608
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10535..10554
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10670..10717
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10736..11110
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11240..11361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11277..11296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11331..11350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11410..11426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11410..11426)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11423..11445)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11434..11450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11458..11464,11465..11482,11483..11488))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11503..11524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11641..11658)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11812..12400)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11892..11911)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12571..13362,13363..13431))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13194..13213
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13432..13536)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagcgatg cggggaggcg gcccaaaggg agatccgact
     2641 cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga
     2701 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
     2761 ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac
     2821 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     2881 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
     2941 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
     3001 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
     3061 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
     3121 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
     3181 gtaatccaga ggttgattac cgataagctt gatatcgaat tcttaactgg caccgggctt
     3241 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
     3301 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
     3361 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
     3421 ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg
     3481 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
     3541 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
     3601 gaccgccacg gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
     3661 cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt
     3721 gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac
     3781 gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatggtgg taccccctgg
     3841 ggagagaggt cggtgattcg gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc
     3901 ttgcagaatg cggaacaccg cgcgggcagg aacagggccc acactaccgc cccacacccc
     3961 gcctcccgca ccgccccttc ccggccgctg ctctcggcgc gccctgctga gcagccgcta
     4021 ttggccacag cccatcgcgg tcggcgcgct gccattgctc cctggcgctg tccgtctgcg
     4081 agggtactag tgagacgtgc ggcttccgtt tgtcacgtcc ggcacgccgc gaaccgcaag
     4141 gaaccttccc gacttagggg cggagcagga agcgtcgccg gggggcccac aagggtagcg
     4201 gcgaagatcc gggtgacgct gcgaacggac gtgaagaatg tgcgagaccc agggtcggcg
     4261 ccgctgcgtt tcccggaacc acgcccagag cagccgcgtc cctgcgcaaa cccagggctg
     4321 ccttggaaaa ggcgcaaatc gattgcaaag atggataaag ttttaaacag agaggaatct
     4381 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     4441 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4501 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4561 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4621 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     4681 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     4741 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     4801 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     4861 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     4921 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     4981 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     5041 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     5101 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     5161 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     5221 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     5281 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     5341 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     5401 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     5461 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     5521 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     5581 tcttccattt caggtgtcgt gacctgcagg atcgtgcggg acgtggacgg aagaaaaaag
     5641 agagtgagcg agcgccggaa tcagcccggc gtggagtgcg gacccgcggt agtgccaggg
     5701 ggcgaaggcg gcggtggtga ggaagatact ttggttagtg accacatcgc agcatgttga
     5761 gtcaagttta ccgctgtggg ttccagccct tcaaccaaca tcttctgccc tgggtcaagt
     5821 gtacaaccgt cttcagatct cattgtatcc agccttcagt aatcagacat gttcgttctt
     5881 ggagcaacat cccgtttatc actgtacccc tcagtcgtac acatggcaag tccttcgccc
     5941 accgcagtga gctgaagcat gccaagagaa tcgtggtgaa gctcggcagt gccgtggtga
     6001 cccgagggga tgaatgtggc ctggccctgg ggcgcttggc atctgggtca gaacctgcaa
     6061 catcaggatc cgagaccccg gggacgtcag agtcagcaac ccccgaatct ggaccaggca
     6121 cgagcactga gccgtcagaa gggtcagcac ctggtagtcc agccggaagt ccaacgagca
     6181 cagaagaggg cacaagtgag tccgcaaccc ccgagagcgg cccaggaagc gagcctgcga
     6241 catccggcag cgagaccccg ggcacctcag aatcagcaac tccggagagt ggaccgggta
     6301 gcccagccgg atctcccact agtacggaag aggggtcccc cgcaggctca cccacgtcca
     6361 ccgaggaggg tacttctacg gaaccttctg aggggtccgc accaggtacc tctgaatctg
     6421 caacgcccga gtccggtcca ggaacgtcag agagtgcaac tccggaatct ggcccgggca
     6481 ccagtgaatc tgccactcct gaatccggtc cgggctctga gccagccaca tcagggtctg
     6541 aaacaccggg gagcgaaccc gcgactagtg gaagtgaaac acctgggtcc ccagccgggt
     6601 ctccgaccag tactgaggag ggaacgtcaa cagaaccatc cgagggtagc gcccctggga
     6661 cttcaacgga gcctagtgaa ggcagcgcgc cggggtctga acccgcgacc agtggtagcg
     6721 agacgccggg cacatctgag tccgccacac cagagtctgg gcccggtacg agtacagaac
     6781 catctgaggg cagtaccttc ttttcagaag taaagcctgc aggccctact gttgagcagc
     6841 agggagaaat ggcgcgatct ggaggaagga tgttggccac cttggaacct gagcagagag
     6901 cagaaattat ccatcatctg gctgatctgt tgacggacca gcgtgatgag atcctgttag
     6961 ccaacaaaaa agacttggag gaggcagagg ggagacttgc agctcctctg ctgaaacgtt
     7021 taagcctctc cacatccaaa ttgaacagcc tggccatcgg tctgcgacag atcgcagcct
     7081 cctcccagga cagcgtggga cgtgttttgc gccgcacccg aatcgccaaa aacttggaac
     7141 tggaacaagt gactgtccca attggagttc tgctggtgat ctttgaatct cgtcctgact
     7201 gtctacccca ggtggcagct ttggctatcg caagtggcaa tggcttgtta ctcaaaggag
     7261 ggaaggaggc tgcacacagc aaccggattc tccacctcct gacccaggag gctctctcaa
     7321 tccatggagt caaggaggcc gtgcaactgg tgaataccag agaagaagtt gaagatcttt
     7381 gccgcctaga caaaatgata gatctgatca ttccacgtgg ctcttcccag ctggtcagag
     7441 acatccagaa agctgctaag gggattccag tgatggggca cagcgaaggg atctgtcaca
     7501 tgtatgtgga ttccgaggcc agtgttgata aggtcaccag gctagtcaga gactctaaat
     7561 gtgaatatcc agctgcctgt aatgctttgg agactttgtt aatccaccgg gatctgctca
     7621 ggacaccatt atttgaccag atcattgata tgctgagagt ggaacaggta aaaattcatg
     7681 caggccccaa atttgcctcc tatctgacct tcagcccctc cgaagtgaag tcactccgaa
     7741 ctgagtatgg ggacctggaa ttatgcattg aagtagtgga caacgttcag gatgccattg
     7801 accacatcca caagtatggc agctcccaca cggatgtcat cgtcacagag gacgaaaaca
     7861 cagcggagtt cttcctgcag cacgtagaca gtgcctgtgt gttctggaat gccagcactc
     7921 gcttttctga tggttaccgc tttggactgg gagctgaagt gggaatcagt acatcgagaa
     7981 tccacgcccg gggaccagta ggacttgagg gactgcttac tactaagtgg ctgctgcgag
     8041 ggaaggacca cgtggtctca gatttctcag agcatggaag tttaaaatat cttcatgaga
     8101 acctccctat tcctcagaga aacaccaact gattagtctg ctgaaacaag ccgcctgaac
     8161 gcgttaagtc gacaaagagc caggaaaacc cgggaatttt ccaaaaggtc ttcacgttaa
     8221 acttgtctta tctcaggaga gagcccgctc ttgtctccca gttcctggta gggtctgcct
     8281 gttggaaagt gtacctggat gcttctgggc tccgtttggc aatagcagtc ttggctgatg
     8341 tgcacagtct ggctcccagc tcaccctttt tttttaaagt aagaaaatag ttgctaccga
     8401 tagggacttt gccaagtcca attatcttct aggattgaaa ggtgcatttt ccccataaaa
     8461 aaggcgagga aaacccatgg ctgctttgtg tcacctcagt gacttacagt cccccttggc
     8521 atttagttgg tactagagcc agtcatcctt aacaaatctt ttcacatttt atttctttca
     8581 catgtagtca tcttcaaaaa ggaaagattt ggaattttag aaaaggggca actcttcttt
     8641 ttagcattct catcagaaag tcacaaaaat cgatggaatc atttccactg ggaagattga
     8701 ccttttgtat ttatttgtgg ggtaaattaa taagcattcc agatgcttgc agcttcctgc
     8761 atccaggaga tgctgtgttc cccgtgatgc agctggaacc caagctgcag caggagatgc
     8821 aagtttcagg atgttcccca ctgagctgga ggaatatcta cagcagtgat gcttgaaatt
     8881 tttgtatgaa ttattttgtc gtcctaccct tttcctccaa aacaaaaatt agaggattat
     8941 tttaatactt tggattcttc cccctttttt gagattaatt aactttaaga ccaatgactt
     9001 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     9061 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     9121 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
     9181 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga
     9241 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
     9301 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
     9361 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9421 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9481 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9541 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     9601 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     9661 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     9721 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     9781 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     9841 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     9901 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     9961 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
    10021 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
    10081 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
    10141 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
    10201 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
    10261 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
    10321 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
    10381 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    10441 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
    10501 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    10561 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    10621 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
    10681 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
    10741 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
    10801 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
    10861 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
    10921 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
    10981 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
    11041 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
    11101 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
    11161 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
    11221 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
    11281 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    11341 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
    11401 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    11461 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    11521 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    11581 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    11641 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    11701 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    11761 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    11821 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    11881 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    11941 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    12001 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    12061 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    12121 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    12181 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    12241 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    12301 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    12361 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    12421 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    12481 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    12541 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    12601 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    12661 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    12721 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    12781 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    12841 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    12901 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    12961 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    13021 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    13081 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    13141 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    13201 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    13261 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    13321 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    13381 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    13441 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    13501 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    13561 tgac
//
