LOCUS       sequence-48889-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RNGTT
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg ctcacaacaa gatcccgccg cggtggctga actgtccccg gcgcggccag
      121 ccggtggcag gaagattctt acctctgaag acaatgttag gaccaagata tgatagtcaa
      181 gttgctgaag aaaatcggtt ccatcccagc atgctctcaa attacctaaa gagcctaaag
      241 gttaaaatgg gcttgttggt ggacctgaca aatacttcaa ggttctatga ccgaaatgac
      301 atagaaaaag aaggaatcaa atatataaaa cttcagtgta aaggacatgg tgagtgccct
      361 accactgaga atactgagac ctttattcgt ctgtgtgagc ggtttaatga aagaaatcca
      421 cctgaactta taggtgttca ttgtactcat ggcttcaatc gcactggttt cctcatatgt
      481 gcctttttgg tggagaaaat ggattggagt atcgaagcag cagttgctac ttttgcccaa
      541 gccagaccac caggaatcta caagggtgat tatttgaagg aactttttcg tcggtatggt
      601 gacatagagg aagcaccacc cccacctcta ttgccagatt ggtgttttga ggatgatgaa
      661 gacgaagatg aggatgagga tggaaagaag gaatcagaaa ccgggtcaag tgcttctttt
      721 ggctaagaca gtaaaggtgg atacggatcc gaa
//
