LOCUS       sequence-488890-       13094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stably expresses a chimeric reporter with a mCherry 
            (1-105aa)-XTEN250 linker-TBRG4(351aa-631aa), flanked by 5' and 
            3'UTRs of ALDH18A1 and no mitochondrial targeting sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13094)
  AUTHORS   Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, 
            Ting AY, Weissman JS
  TITLE     Proximity-specific ribosome profiling reveals the logic of localized
            mitochondrial translation.
  JOURNAL   Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 
            10.1016/j.cell.2025.08.002.
  PUBMED    40876456
REFERENCE   2  (bases 1 to 13094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002."
COMMENT     Sequence Label: pJL403
FEATURES             Location/Qualifiers
     source          1..13094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2610..3198
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2716..2727
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3125..3145
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3223..3825)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3309..3329)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3806..3825
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3837..4337)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3900..3918)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        4424..5602
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4655..5593
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5550..5570
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(5897..5915)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     LTR             8899..9079
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9105..9122)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9111..9335
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9381..9809
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9468..9487)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9678..9699
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9818..9838)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9823..10152
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10003..10138
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10065..10084
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10200..10247
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10266..10640
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10770..10891
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10807..10826)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10861..10880
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10940..10956)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10940..10956)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10953..10975)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10964..10980
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10988..10994,10995..11012,11013..11018))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11033..11054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11171..11188)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11342..11930)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11422..11441)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12101..12892,12893..12961))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12724..12743
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12962..13066)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagcgatg cggggaggcg gcccaaaggg agatccgact
     2641 cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga
     2701 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
     2761 ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac
     2821 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     2881 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
     2941 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
     3001 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
     3061 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
     3121 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
     3181 gtaatccaga ggttgattac cgataagctt gatatcgaat tcttaactgg caccgggctt
     3241 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
     3301 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
     3361 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
     3421 ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg
     3481 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
     3541 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
     3601 gaccgccacg gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
     3661 cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt
     3721 gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac
     3781 gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatggtgg taccccctgg
     3841 ggagagaggt cggtgattcg gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc
     3901 ttgcagaatg cggaacaccg cgcgggcagg aacagggccc acactaccgc cccacacccc
     3961 gcctcccgca ccgccccttc ccggccgctg ctctcggcgc gccctgctga gcagccgcta
     4021 ttggccacag cccatcgcgg tcggcgcgct gccattgctc cctggcgctg tccgtctgcg
     4081 agggtactag tgagacgtgc ggcttccgtt tgtcacgtcc ggcacgccgc gaaccgcaag
     4141 gaaccttccc gacttagggg cggagcagga agcgtcgccg gggggcccac aagggtagcg
     4201 gcgaagatcc gggtgacgct gcgaacggac gtgaagaatg tgcgagaccc agggtcggcg
     4261 ccgctgcgtt tcccggaacc acgcccagag cagccgcgtc cctgcgcaaa cccagggctg
     4321 ccttggaaaa ggcgcaaatc gattgcaaag atggataaag ttttaaacag agaggaatct
     4381 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     4441 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4501 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4561 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4621 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     4681 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     4741 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     4801 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     4861 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     4921 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     4981 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     5041 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     5101 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     5161 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     5221 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     5281 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     5341 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     5401 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     5461 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     5521 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     5581 tcttccattt caggtgtcgt gatctagaat cgtgcgggac gtggacggaa gaaaaaagag
     5641 agtgagcgag cgccggaatc agcccggcgt ggagtgcgga cccgcggtag tgccaggggg
     5701 cgaaggcggc ggtggtgagg aagatacttt ggttagtgac cacatcgcag catggtgagc
     5761 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     5821 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     5881 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     5941 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     6001 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     6061 ttcgaggggt cagaacctgc aacatcagga tccgagaccc cggggacgtc agagtcagca
     6121 acccccgaat ctggaccagg cacgagcact gagccgtcag aagggtcagc acctggtagt
     6181 ccagccggaa gtccaacgag cacagaagag ggcacaagtg agtccgcaac ccccgagagc
     6241 ggcccaggaa gcgagcctgc gacatccggc agcgagaccc cgggcacctc agaatcagca
     6301 actccggaga gtggaccggg tagcccagcc ggatctccca ctagtacgga agaggggtcc
     6361 cccgcaggct cacccacgtc caccgaggag ggtacttcta cggaaccttc tgaggggtcc
     6421 gcaccaggta cctctgaatc tgcaacgccc gagtccggtc caggaacgtc agagagtgca
     6481 actccggaat ctggcccggg caccagtgaa tctgccactc ctgaatccgg tccgggctct
     6541 gagccagcca catcagggtc tgaaacaccg gggagcgaac ccgcgactag tggaagtgaa
     6601 acacctgggt ccccagccgg gtctccgacc agtactgagg agggaacgtc aacagaacca
     6661 tccgagggta gcgcccctgg gacttcaacg gagcctagtg aaggcagcgc gccggggtct
     6721 gaacccgcga ccagtggtag cgagacgccg ggcacatctg agtccgccac accagagtct
     6781 gggcccggta cgagtacaga accatctgag ggcagtgagg cctttgccca gcacgtcctg
     6841 aacagagcgc aggacatcac cctgccccac ctgtgcagcg tacttctggc ttttgcgcgt
     6901 ctgaacttcc atccagacca agaggatcag ttcttcagcc tggtacatga gaagctgggg
     6961 tcagagctgc caggcctgga gccagccctg caggtggacc tggtgtgggc cctgtgtgtg
     7021 ctgcagcagg cacgggaagc agagctgcaa gccgtcctcc accctgaatt tcacatccaa
     7081 tttctagggg gcaagtctca gaaggatcag aacaccttcc agaagctgct ccacatcaac
     7141 gccactgccc tgctggagta ccccgagtac tcgggtcccc ttctgcctgc ctcggctgtg
     7201 gcccctgggc cctcagccct tgacaggaag gtgacccccc tgcaaaagga gctgcaggag
     7261 acgctgaagg ggctgctggg gagcgccgac aagggcagcc tcgaggtggc cacgcagtat
     7321 ggctgggtgc tggatgctga ggtgctgctg gacagtgacg gcgagtttct gcccgtaagg
     7381 gactttgtgg cacctcacct tgcccagcca actgggagcc agtcaccacc tccagggtct
     7441 aagaggctag cgttcttgcg gtgggagttc cccaacttca acagccgaag caaggacttg
     7501 ctgggtcgct ttgttctggc ccggcgacac atagtggctg caggcttcct gatagtggac
     7561 gtcccattct atgagtggct ggaactcaag tctgaatggc agaaaggcgc ctacctcaag
     7621 gacaagatgc gcaaagcggt ggctgaggag ctggccaagt gattagtctg ctgaaacaag
     7681 ccgcctgaac gcgttaagtc gacaaagagc caggaaaacc cgggaatttt ccaaaaggtc
     7741 ttcacgttaa acttgtctta tctcaggaga gagcccgctc ttgtctccca gttcctggta
     7801 gggtctgcct gttggaaagt gtacctggat gcttctgggc tccgtttggc aatagcagtc
     7861 ttggctgatg tgcacagtct ggctcccagc tcaccctttt tttttaaagt aagaaaatag
     7921 ttgctaccga tagggacttt gccaagtcca attatcttct aggattgaaa ggtgcatttt
     7981 ccccataaaa aaggcgagga aaacccatgg ctgctttgtg tcacctcagt gacttacagt
     8041 cccccttggc atttagttgg tactagagcc agtcatcctt aacaaatctt ttcacatttt
     8101 atttctttca catgtagtca tcttcaaaaa ggaaagattt ggaattttag aaaaggggca
     8161 actcttcttt ttagcattct catcagaaag tcacaaaaat cgatggaatc atttccactg
     8221 ggaagattga ccttttgtat ttatttgtgg ggtaaattaa taagcattcc agatgcttgc
     8281 agcttcctgc atccaggaga tgctgtgttc cccgtgatgc agctggaacc caagctgcag
     8341 caggagatgc aagtttcagg atgttcccca ctgagctgga ggaatatcta cagcagtgat
     8401 gcttgaaatt tttgtatgaa ttattttgtc gtcctaccct tttcctccaa aacaaaaatt
     8461 agaggattat tttaatactt tggattcttc cccctttttt gagattaatt aactttaaga
     8521 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     8581 gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca
     8641 caaggctact tccctgattg gcagaactac acaccagggc cagggatcag atatccactg
     8701 acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat
     8761 gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     8821 agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag
     8881 ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8941 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     9001 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     9061 gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     9121 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     9181 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     9241 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     9301 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     9361 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     9421 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     9481 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     9541 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     9601 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     9661 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     9721 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     9781 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     9841 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     9901 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9961 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
    10021 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
    10081 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    10141 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
    10201 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
    10261 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
    10321 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
    10381 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
    10441 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
    10501 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
    10561 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
    10621 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
    10681 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
    10741 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
    10801 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    10861 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
    10921 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
    10981 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
    11041 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
    11101 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
    11161 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    11221 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    11281 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    11341 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    11401 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    11461 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    11521 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    11581 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    11641 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    11701 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    11761 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    11821 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    11881 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    11941 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    12001 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    12061 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    12121 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    12181 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    12241 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    12301 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    12361 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    12421 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    12481 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    12541 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    12601 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    12661 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    12721 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    12781 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    12841 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    12901 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    12961 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    13021 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    13081 aagtgccacc tgac
//
