LOCUS       sequence-488936-       12559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary Plasmid for plant transformation expressing RUBY from the 
            Lactuca sativa polyubiquitin 4 gene promoter and the terminator from
            the same gene. Kanamycin plant selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12559)
  TITLE     Monitoring the stability of transgene expression in lettuce using 
            the RUBY reporter.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLSUBR
FEATURES             Location/Qualifiers
     source          1..12559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(59..311)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(324..1118)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(435..454)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1045..1064
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1140..1319)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             complement(2022..5972)
                     /label=RUBY
     CDS             complement(3525..3581)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(4416..4472)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    7923..7947
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8256..8885
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9314..10387
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10453..10647
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        10709..10813
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10814..11608
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11779..12367
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12268..12287
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    12503..12527
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gcggacgttt ttaatgtact ggggttgaac actctgtgcc gaattcggat ccagcgtcga
       61 tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg
      121 ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata
      181 aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat
      241 atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg
      301 tttgaacgat ctgcttgaca agctcagaag aactcgtcaa gaaggcgata gaaggcgatg
      361 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
      421 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
      481 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
      541 aagcaggcat cgccctgggt cacgacgaga tcctcgccgt cgggcatccg cgccttgagc
      601 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
      661 acaagaccgg cttccatccg agtacgtcct cgctcgatgc gatgtttcgc ttggtggtcg
      721 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
      781 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
      841 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
      901 gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgga gttcattcag ggcaccggac
      961 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     1021 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     1081 gccggagaac ctgcgtgcaa tccatcttgt tcaatcattt gagagtgaat atgagactct
     1141 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     1201 gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     1261 gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg ttgcggttct
     1321 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     1381 ccttaattct ccgctctccg caagaattca agcttagcga acgcgggcta taatctagta
     1441 aaactaaagt gtaactaata gtggatttac aaaaattata tgtaaaagac caaaactgca
     1501 aattaatatg acttcgaact catgaattat atagggaaat aacctttaat taggatatta
     1561 aattggttta gttttatcta atggatatct atttcgatta ttgtgttaga tttgattcaa
     1621 taaaatatct aactgttatc caatttgtgt gcaattacaa ccgacaaata tttatattta
     1681 taattgaaaa tattatagtt ataaatgcct tataagtgga catatccaac ttgatgaaaa
     1741 ttggatattt aattatccga taattagata aagaattgta ttatatttgg ttgggttcaa
     1801 aacgttacca aaaacttaac ctcaaatgcg caatttgaca acacaaagac aatgaacaat
     1861 taaaataaca tggcttaatc aatttcacag aaaaccaaag tttttttatt caacaattat
     1921 tcacttcaaa ccatcaaacc agagtttcaa tcacaacaca aacattcaaa catacacaaa
     1981 ttcattcatc aacaaccttc ataacaccac caaaccaaag ctcactggag gcttggctca
     2041 agtttggcct gcatgacggc gtcgaggaaa tcgtcgatcg ccttcacgga ggagcccttc
     2101 agctcttttt cctcgttcac ggcgtccttg agctttttgc ccatttccac ggcgcgattc
     2161 ttcatctccc agcctttgga gccctcgttg cgctcaagga tcatctcgac gatgcgcttg
     2221 accttctctt tggagatctc gccttcgagg cctctagcaa gctcaaccgc gacgcccatc
     2281 tcatcttcga ggtacttgag gttgtaggcc tgctcggcag caagtggcca tccaagcatc
     2341 ggaacgccct cgcgaagaga ctcaaggatg ctgttccatc cgcagtgggt gagaaagccg
     2401 ccgatggatt catggttgag gatctcaagc tgcgggccga gcttgtacac gagcttgccc
     2461 tgtttcttca ccttcatgcg ttcctcaaag ccctctggca gccactctgg gcgcatctct
     2521 tcgttgatgt cgaagccgaa cggggctctg atcacccaga ggaatggctt ctcggaggac
     2581 tcaaggccag cagcgagttc catcatctgc gtcgggctga tcgtgttctg ggagccgaag
     2641 gagatgtaga ggacggagtc cggctctttg aggctgagcc actgcacgaa ctcggcgcct
     2701 gttgaattgt tgtcggagga ggagtgctgc actggggaag caataagtgg gccgatgccc
     2761 cagatcggca gcttggtgta gttgcggagg atggagaagc cgagtgtctc gatctcttcc
     2821 acggagttgc agagccagcc aaaagacttc atggactggc ggagctgcgg ctggaaatac
     2881 ttggaccaat cgtcggagcc gtcggcatat ctgaggaacc gatggagctg ggacctgcgg
     2941 aacttatggt tctctgggaa gcctggcagc gggaactctt ggtcgtcgga gtagttctgg
     3001 tgcgggagat cgttccagat ggacacgtag gcggatgtgc cataagcgcc gcctgttgtg
     3061 aacaccacgc ctgtagagcc cacatccttg gccacctggt caacccagcc gaggaacaca
     3121 tcatggatca cgcagagtgg tggcctggct gggctgaaat gcctggagat gtaatcgcgg
     3181 aggtgcggat cgaggctgat ggtggaatgg aagagggaca ccacgagcgg gagtgtgagc
     3241 ttatcggtgt tctcgatgcc cggtgggagt ccgtggttgg tggagttgaa tggcagctcg
     3301 acgatcctga tgccgctgga ggagtaggaa tggtggtgaa ggaggtgcct gaggaagccg
     3361 gcattgagcg gagttgtgag cagggtgatg atcacgtggg agcgcttgta gaggaacatg
     3421 gcgagttcga gaaatggcct gaggtggccc tgcgccatga acgggatcat gaggatgtgc
     3481 tgtgtctcgc cctcgccgtt ggtgttcatc ttgatggcgg tcatggggcc aggattttcc
     3541 tcaacatctc ctgcttgctt gagcaggcta aagttggttg ctccggatcc ggcggaggtg
     3601 aacttgtagg agccgtggca aagtgtgcca tgatcccagg aggagtggat cagctcggcc
     3661 ttccactttt caccagcagc gccgagcaca acgtggagcg ggtagaagtg ctctggaaat
     3721 ggatgggcga gcttccagtt cggggccttg ctctcgtaga tgttcacttc ctcgaagcgg
     3781 ccgttgatga gggccttcct aagccaagaa tcgaaggcag cggcccaagg ggcaacacca
     3841 tcgaagtagt gcggtgtctc atcgagtgga tgtgtggcgc tgccggagcc aataatgagc
     3901 acgccatcat tcttgagcgg cgcgagggct ctgccgagat tgtagtggta ggtgccgtcg
     3961 agatgtggct gaactgagag ctggcacacc gggatgtccg cctctggata catgagcatg
     4021 agtggaaccc aggcgccgtg atcaaggccc ctcttttggt ctgtctcggc tgtctcgaac
     4081 ccggacttct tgaggatctc ttccaccttt ctcgcaagct ctggctcgcc tggagctggg
     4141 tacttgaact ggtacatggc ggctgggtag tcgtcgaagt cgtggatggt gtcgttgatg
     4201 tgcacggcgt tcacggttgg cttcactgtc tcccagtggc cggagatgat gaggatggcc
     4261 ttcggcttct tggagaaaat cttctcgcgc catgtctcga agaacggcct gagcggatgt
     4321 gtatcctcga cggtgaggat cgggttgccg tgggtgatga agaaggactc tttgatcatc
     4381 tggtcgttgg cgtcctcgcc gttcatcatc ttcataggcc cggggttctc ttctacatca
     4441 cctgcctgct taaggaggct aaaattggta gctccgctac cgtagcgcgg aatcgggatg
     4501 agcttgagcg gcttggtctt ttggagcgca atgccgaact tctcgtccat gtcgaggtcc
     4561 ttcggggaga tgtcgccttc gagcttccag ttgaagaact ggaggagtgt ggcgagcatg
     4621 agggtgagca tcctgatggc gagattcatg cctgggcaaa ttctgcggcc agcgccaaat
     4681 ggaaggaggc cgaaatcgcg gcccttaaca tcgatctcgc agccgatgaa gcgctctggg
     4741 ctgaaaatat cggcgttctg ccaggcgttt gggtccctgc caatggccca gaggttcacg
     4801 aggatctggg catccttcgg cacgatgtag ccgtacagct cgacatcggt gtcggccttt
     4861 cttgggagca agaacacggt cggcggatgg aggcggagtg tctctttgat gatcgcctgg
     4921 aggtacggca ggttgatgat gtcggactct tggatctgct tgtccttgcc gaggacctgc
     4981 ttgatttcct cttgggcctt ttccatcatc tctggattgc ggatcagctc ggtcatcacc
     5041 cactcgaatg tggaggatgt ggtatcggtg ccggcgtcga agatgtccac gaggaggtgg
     5101 ttgatctcgc ccatcgtcag ctcgttctgc ttgaacagct ggaggagcac atcgagcacg
     5161 tcgtcggtgg tggtggtagt tgtggtggag gaatctgggg cgagtctctc gcagatgatg
     5221 ccctggaaca cggcgatcag cttgtcgaag gagcaggcga gccttcttct aatgccagat
     5281 gggtcaacgc agccgagaat cgggaagtag tcggcgtagt tcggcttgcc tatatcttcc
     5341 atgatgttcc agatcagctc tttgaactct tggctggtat gggacttgtg gtgcgccagc
     5401 tcgacgctga agaacagctt gctgaggagg ttgaggctgg tcgtaaaggc ggccttgcca
     5461 atatccacgg cctggccttt ctgggcgcac tcttgaacgt actcgtagag ctgctgcacc
     5521 ttggcgtgcc tgaatgtctg gcaggcatcg agtctctgtg gggagagcag atgcacggcg
     5581 gtaatcttgc ggaagttgcg ccacttcgga gacaccggga gccaagacat ggtgagcttg
     5641 tggtggtcgc cggctgtaac actattcggg atcgtgcggt tggagagcgg gtgatccttc
     5701 ttgaggaaca tctctttggc cacgtcggca gaggacacaa cgatggtggt cacagagccg
     5761 agtctgaggg aaatgagtgg gccgtgaatc ttggcgaggt tggcgaagga ccgatgcggc
     5821 ttcttgccca cctcaaggat gttgccgatg atcggaagcg gctttggtcc tggcggaagc
     5881 agcttggtgg tctgctggga gaacagcagc ttgatgaagt ggaagctgat gaaccagatc
     5941 gcgaggatca tggcgagggt cgcatgatcc atcattctgt taaaaaaaga acgaaacaat
     6001 tattagaaaa cgaaaacagt tttatcatca attcatcaac aaacaaattc atatcgtact
     6061 tgaaacagag atcagattac aattcaaaaa caaatcaaca actttattct ctgaaattga
     6121 aaacttagat cgtgaattaa tttacaaaaa atcgtcgttc gaagctttta tacaataaca
     6181 tcgattttgg aattgacttg ttgaaaaacg atcgaaaaca cgaaactata acagatcgtt
     6241 aataaattcg atgattagat ctagaaacca taattaaaac atattaagac actatcaatc
     6301 gtgttaatta caacatcagg ttatatctta aatcaaaagc cacaaacaga tcaaaaacaa
     6361 taataacctt taatcgattg aaaataaact gttcaaaaac taaacaaaca taaaagaata
     6421 aaaaagataa taccttggga gaattgaaga agtcagagct ttgaagataa agatgattgc
     6481 aggatgaagg aagatgatgg attgggggtg gatttatagg ataagaaaag gtttcttcac
     6541 gaaaaggaag gctaacgcta cgggttgtta gataacaaac ggacgaaaat atgacacgtg
     6601 tcaataaaaa gttgatgaag ggtctgaaat tagagtcgtt ggattccgat atttaatcag
     6661 tggcggtgat ttctaaggct gccgtcatct tttgggttag gataatatct tttctttgtg
     6721 tttcgtccaa gaaactttta acttaaatct tgagtcgatg ggatatttgg ttttggatcc
     6781 atataatttg taaattttat aatttaagga atacaagatt aggttaatct ctttgaaaag
     6841 ttagtaataa ataattagag aatagagtat gttattgata ttgaaaatac ttttattttt
     6901 taatttttta ttttggaaaa gaaactttct aacaagggtt tttttgccct gcttttgcac
     6961 gccgaataat ctttcgctgc aaatatgagg ttagtagttg tgatacttgt atattatacg
     7021 ggttgattac aataaaatgt taaatacgaa tgattaaaca gcaaatttat ttgaatttga
     7081 aatttggaat aagtaagaat tataaaaaaa aaatgaaaaa aataaattaa aattatgtgt
     7141 ttagttatct gactcgtgta tcaaatgtct aattataaca aaatgttaaa tacaaacgtt
     7201 tcaattgtaa atttatttaa agtttcgatt gaagtttgaa attcaaaatt taaaattaat
     7261 atagttatta tgatatggtt aatttataaa aactaaatta aggatatatt caaaaataaa
     7321 aagtgaagta aatgacaaat gaaaaataaa tatctcaaaa ttatgacaaa atgacatttt
     7381 gtataatgca tgagagtgtg acaagtgaca aaaaaaaact ttaatttatt aaagaggata
     7441 cttcatgttt tggagtcaat gccgacaaac atttatggcc acaggaccga gtagtgtcaa
     7501 aatttgaaca ttgatcaata agttatacat catatatttc atatgtttta ctacgtcacc
     7561 acaaaccaaa tgattatatt gaaaatactt ttaaattata caaaataagt ttgatttgga
     7621 tggataaatt ttataatata aggaataaaa gattaggtga aactttatga aaatttatta
     7681 taaataatca gataataaag tatgtttgta tattggaaat gattttaaat taaataacac
     7741 agggaaattg agtattctaa cccatataaa tgaataattt gaaaaaaaat gattattttt
     7801 tttgtaaaat aaccattttg tcgggaatat atcactttag agaggtcact gaaaaaccgc
     7861 tccactagag gatgcacatg tgaccgaggg acacgaagtg atccgtttaa actatcagtg
     7921 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
     7981 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
     8041 cagggttccc ctcgggagtc agccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     8101 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     8161 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     8221 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     8281 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     8341 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     8401 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     8461 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     8521 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     8581 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
     8641 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
     8701 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
     8761 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
     8821 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
     8881 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
     8941 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
     9001 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
     9061 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
     9121 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
     9181 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
     9241 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
     9301 ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct
     9361 gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga
     9421 ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc
     9481 ccggcaaccg ccggcagccg gtgcgtcgtc gattaggaag ccgcccaagg gcgacgagca
     9541 accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat
     9601 ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta
     9661 cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg
     9721 ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg
     9781 ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa
     9841 gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg
     9901 gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt
     9961 gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg
    10021 gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga ttacagaagg
    10081 caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg
    10141 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt
    10201 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt
    10261 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca
    10321 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg
    10381 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa
    10441 aagcttcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg
    10501 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac
    10561 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac
    10621 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aacagctgca
    10681 aaaagcgccc acatcaaggc tccgagtgcg cggaacccct atttgtttat ttttctaaat
    10741 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
    10801 aaaaaggaag agtatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
    10861 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
    10921 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
    10981 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
    11041 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
    11101 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
    11161 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
    11221 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
    11281 cgaaaactgg gaagaggaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
    11341 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
    11401 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
    11461 caagtggtat gacattgcct tctgcgtccg gtcgctcagg gaggatatcg gggaagaaca
    11521 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
    11581 ttatatttta ctggatgaat tgttttagct gtcagaccaa gtttactcat atatacttta
    11641 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    11701 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    11761 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    11821 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    11881 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    11941 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    12001 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    12061 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    12121 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    12181 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    12241 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    12301 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    12361 atggaaaaac gccagcaacg cggccttttt acggttcctg ctcggatctg ttggaccgga
    12421 cagtagtcat ggttgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
    12481 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
    12541 gacaacttaa taacacatt
//
