LOCUS       sequence-488964-        9106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of ACTA2-M178V cDNA from a lentiviral 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9106)
  AUTHORS   Alves CRR, Das S, Krishnan V, Ha LL, Fox LR, Stutzman HE, Shamber 
            CE, Kalailingam P, McCarthy S, Lino Cardenas CL, Fong CE, Imai T, 
            Mitra S, Yun S, Wood RK, Benning FMC, Roh K, Lawton J, Kim N, 
            Silverstein RA, Ferreira da Silva J, de la Cruz D, Richa R, Xie J, 
            Gray-Edwards HL, Malhotra R, Chung DY, Chao LH, Tsai SQ, Maguire CA,
            Lindsay ME, Kleinstiver BP, Musolino PL
  TITLE     Treatment of a severe vascular disease using a bespoke CRISPR-Cas9 
            base editor in mice.
  JOURNAL   Nat Biomed Eng. 2025 Sep 11:10.1038/s41551-025-01499-1. doi: 
            10.1038/s41551-025-01499-1.
  PUBMED    40935887
REFERENCE   2  (bases 1 to 9106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed 
            Eng. 2025 Sep 11:10.1038/s41551-025-01499-1. doi: 
            10.1038/s41551-025-01499-1."
COMMENT     Sequence Label: pCMV-pLenti-ACTA2-M178V  (LLH709)
FEATURES             Location/Qualifiers
     source          1..9106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2976
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2999..3302
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3303..3506
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3457..3477
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3503..3527
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3623..3647
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4779..4803)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4856..4897
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4921..5509
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4974..4994)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5392..5403)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5524..5840
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5691..5826
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(5891..5893,5894..5896,5897..6610)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5936..5957)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6197..6216)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6544..6565
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6682..6915
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6987..7108
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7024..7043)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7078..7097
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7148..7283
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7303..7322)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7304..7322)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7332..7349)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7332..7348)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7341..7363)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7490..7945
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7577..7596)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7787..7808
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7971..8075
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8076..8144,8145..8936)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8294..8313)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg gagttccgcg
     3001 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     3061 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     3121 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     3181 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     3241 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     3301 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     3361 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     3421 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     3481 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     3541 atccacgctg ttttgacctc catagaagac accgactcta gaggatccac tagtccagtg
     3601 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gttggcatgt gtgaagaaga
     3661 ggacagcact gccttggtgt gtgacaatgg ctctgggctc tgtaaggccg gctttgctgg
     3721 ggacgatgct cccagggctg ttttcccatc cattgtggga cgtcccagac atcagggggt
     3781 gatggtggga atgggacaaa aagacagcta cgtgggtgac gaagcacaga gcaaaagagg
     3841 aatcctgacc ctgaagtacc cgatagaaca tggcatcatc accaactggg acgacatgga
     3901 aaagatctgg caccactctt tctacaatga gcttcgtgtt gcccctgaag agcatcccac
     3961 cctgctcacg gaggcacccc tgaaccccaa ggccaaccgg gagaaaatga ctcaaattat
     4021 gtttgagact ttcaatgtcc cagccatgta tgtggctatc caggcggtgc tgtctctcta
     4081 tgcctctgga cgcacaactg gcatcgtgct ggactctgga gatggtgtca cccacaatgt
     4141 ccccatctat gagggctatg ccttgcccca tgccatcgtg cgtctggatc tggctggccg
     4201 agatctcact gactacctca tgaagatcct gactgagcgt ggctattcct tcgttactac
     4261 tgctgagcgt gagattgtcc gggacatcaa ggagaaactg tgttatgtag ctctggactt
     4321 tgaaaatgag atggccactg ccgcatcctc atcctccctt gagaagagtt acgagttgcc
     4381 tgatgggcaa gtgatcacca tcggaaatga acgtttccgc tgcccagaga ccctgttcca
     4441 gccatccttc atcgggatgg agtctgctgg catccatgaa accacctaca acagcatcat
     4501 gaagtgtgat attgacatca ggaaggacct ctatgctaac aatgtcctat cagggggcac
     4561 cactatgtac cctggcattg ccgaccgaat gcagaaggag atcacggccc tagcacccag
     4621 caccatgaag atcaagatca ttgcccctcc ggagcgcaaa tactctgtct ggatcggtgg
     4681 ctccatcctg gcctctctgt ccaccttcca gcagatgtgg atcagcaaac aggaatacga
     4741 tgaagccggg ccttccattg tccaccgcaa atgcttctaa ccaactttct tgtacaaagt
     4801 ggttgatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa
     4861 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgatcgac
     4921 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4981 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     5041 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     5101 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     5161 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     5221 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     5281 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     5341 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     5401 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     5461 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggcgatggt
     5521 accggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5581 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5641 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     5701 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5761 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5821 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     5881 agcacctagc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     5941 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     6001 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     6061 ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag tgcttcgccc gctaccccga
     6121 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     6181 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     6241 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     6301 cctggggcac aagctggagt acaactacaa cagccacaag gtctatatca ccgccgacaa
     6361 gcagaagaac ggcatcaagg tgaacttcaa gacccgccac aacatcgagg acggcagcgt
     6421 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     6481 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     6541 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     6601 gtacaagtaa ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6661 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     6721 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6781 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6841 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6901 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     6961 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     7021 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7081 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     7141 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7201 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7261 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     7321 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     7381 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     7441 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     7501 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7561 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7621 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7681 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7741 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7801 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7861 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7921 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7981 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8041 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8101 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8161 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8221 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     8281 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     8341 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8401 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8461 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8521 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8581 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8641 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8701 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8761 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8821 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8881 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8941 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9001 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9061 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
