LOCUS       sequence-48901-e        1452 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1452)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HMGCS1
FEATURES             Location/Qualifiers
     source          1..1452
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg atgtgggaat tgttgccctt gagatctatt ttccttctca atatgttgat
      121 caagcagagt tggaaaaata tgatggtgta gatgctggaa agtataccat tggcttgggc
      181 caggccaaga tgggcttctg cacagataga gaagatatta actctctttg catgactgtg
      241 gttcagaatc ttatggagag aaataacctt tcctatgatt gcattgggcg gctggaagtt
      301 ggaacagaga caatcatcga caaatcaaag tctgtgaaga ctaatttgat gcagctgttt
      361 gaagagtctg ggaatacaga tatagaagga atcgacacaa ctaatgcatg ctatggaggc
      421 acagctgctg tcttcaatgc tgttaactgg attgagtcca gctcttggga tggacggtat
      481 gccctggtag ttgcaggaga tattgctgta tatgccacag gaaatgctag acctacaggt
      541 ggagttggag cagtagctct gctaattggg ccaaatgctc ctttaatttt tgaacgaggg
      601 cttcgtggga cacatatgca acatgcctat gatttttaca agcctgatat gctatctgaa
      661 tatcctatag tagatggaaa actctccata cagtgctacc tcagtgcatt agaccgctgc
      721 tactctgtct actgcaaaaa gatccatgcc cagtggcaga aagagggaaa tgataaagat
      781 tttaccttga atgattttgg cttcatgatc tttcactcac catattgtaa actggttcag
      841 aaatctctag ctcggatgtt gctgaatgac ttccttaatg accagaatag agataaaaat
      901 agtatctata gtggcctgga agcctttggg gatgttaaat tagaagacac ctactttgat
      961 agagatgtgg agaaggcatt tatgaaggct agctctgaac tcttcagtca gaaaacaaag
     1021 gcatctttac ttgtatcaaa tcaaaatgga aatatgtaca catcttcagt atatggttcc
     1081 cttgcatctg ttctagcaca gtactcacct cagcaattag cagggaagag aattggagtg
     1141 ttttcttatg gttctggttt ggctgccact ctgtactctc ttaaagtcac acaagatgct
     1201 acaccggggt ctgctcttga taaaataaca gcaagtttat gtgatcttaa atcaaggctt
     1261 gattcaagaa ctggtgtggc accagatgtc ttcgctgaaa acatgaagct cagagaggac
     1321 acccatcatt tggtcaacta tattccccag ggttcaatag attcactctt tgaaggaacg
     1381 tggtacttag ttagggtgga tgaaaagcac agaagaactt acgctcggcg tccctgacag
     1441 taaaggtgga ta
//
