LOCUS       sequence-489025-       11097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector with nuclear fluorescent reporter AcGFP expressed 
            from Rat NSE promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11097)
  AUTHORS   Delzor A, Dufour N, Petit F, Guillermier M, Houitte D, Auregan G, 
            Brouillet E, Hantraye P, Deglon N
  TITLE     Restricted transgene expression in the brain with cell-type specific
            neuronal promoters.
  JOURNAL   Hum Gene Ther Methods. 2012 Aug;23(4):242-54. doi: 
            10.1089/hgtb.2012.073. Epub 2012 Aug 30.
  PUBMED    22934828
REFERENCE   2  (bases 1 to 11097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/hgtb.2012.073"; journalName: "Hum Gene Ther Methods"; date:
            "2012-08"; volume: "23"; issue: "4"; pages: "242-54"
COMMENT     Sequence Label: SIN-rNSE-AcGFPnuc-WPRE
FEATURES             Location/Qualifiers
     source          1..11097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..80
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     62..79
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             105..338
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    427..1015
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             533..544
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     942..962
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     1028..1044
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1029..1045
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1068..1092
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1126..1146)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(1150..1170)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(1174..1194)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(1219..1935)
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     regulatory      1932..1941
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2000..2024)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2045..2054
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3904..3928)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(4236..4280)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4465..4698
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5191..5316
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5363..5996
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6164..6298
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6188..6207)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6242..6261
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6723..6743)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6873..6938
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(8072..8401)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8086..8221
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8386..8406
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(8476..8493)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8647..9235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8727..8746)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9406..10197,10198..10266))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10029..10048
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10267..10371)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10439..10457
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10495..10517)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10617..10636
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10850..10869
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 acatattgtc gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac
       61 gatttaggtg acactataga atacacggaa ttaattctag atgctgctag agattttcca
      121 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
      181 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
      241 gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac aagcaaaaag
      301 cagatcttgt cttcgttggg agtgaattag cccttccagt cccccctttt cttttaaaaa
      361 gtggctaaga tctacagctg ccttgtaagt cattggtctt aaaggtaccg agctcgaatt
      421 cccgatgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac
      481 gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg
      541 ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca
      601 gccatggaaa ggacgtcagc ttccccgaca acaccacgga attgtcagtg cccaacagcc
      661 gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga
      721 gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg
      781 gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact
      841 cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg
      901 aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa
      961 catagttaag aataccagtc aatctttcac aaattttgta atccagaggt tgattatcgg
     1021 aattccctcg aggtcgacgg tatcgataag cttgatatcg aattatcacc actttgtaca
     1081 agaaagctgg gtctagatat ctcgagtgcg gccgcgaatt gatcctacct ttctcttctt
     1141 ttttggatct acctttctct tcttttttgg atctaccttt ctcttctttt ttggatcagc
     1201 tcgagatctg agtccggact tgtacagctc atccatgccg tgggtgatgg cggcggcggt
     1261 cacgaagccg aagtagatca tgtgatcgcg cttctcgttg gggtccttgg acagggcgct
     1321 ctgggtggac aggtagtggt tatcgggcag cagcacaggg ccatcgccga tgggggtatt
     1381 ctgctggtag tggtcggcca gctgcacgct gccatcctcg atgttgtggc ggatcttgaa
     1441 gttcaccttg atgccattct tggccttgtc ggtcatgatg tacacattgt gggcgttgta
     1501 gttgtactcc atcttattgc ccaggatgtt gccatcctcc ttgaaatcgg tgccggtcag
     1561 ctcgatgcga ttcaccaggg tatcgccctc gaacttcacc tcggcgcgcg acttgtagtt
     1621 gccgtcatcc tcgaagaaga tggtgcgctc ctggatgtag ccctcaggca tggcgctctt
     1681 gaagaagtcg tgctgcttca tgtgatcggg gtagcgtgag aagcactgca cgccgtagct
     1741 cagggtggtc accagggtgg gccagggcac aggcagcttg ccggtggtgc agatgaactt
     1801 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccgctcacgc tgaacttgtg
     1861 gccattcaca tcgccattca gctcgatcag gatgggcacg atgccggtga acagctcggc
     1921 gcccttgctc accatggtgg cgaccggtag cgctagattc ggtaccggat ccagtcgact
     1981 gaattggttc ccatggtgga gcctgctttt ttgtacaaac ttgtgataat tcctgcagcc
     2041 cgatccatgg tggcgaccgg tggatctcga ggactgcaga ctcagccggt ggcggtggcg
     2101 gtggtgacgg cggcggcgca gagagagcag gagtggcggc ggctgcggct cccagcgcgg
     2161 gcggggggtg ggcagggggc ggcgcctata gagccgagcg agcgcatgtg acccgggggc
     2221 cccgatgagt caagagctgc aggcggagac gcacgtacga gcgcgggtgg tggtggggaa
     2281 ccgaacgggt gggggaaggg cccgggcgat ccaggaccaa gctggcaacc ccataagtac
     2341 acggatgctc ataaccttgt ccagccaact cgagggaagc aggcgtcccc ttattccagt
     2401 atagtggcag gtactaaccc agacagatgg agaagcccac cacaggggtt cacgaactcg
     2461 acacagaata taaaaacgcc tttttatttg aaagctgaac tgatccatca cagcccacct
     2521 cctcggctct tctctccaga caacccctac tagcatctta gtcgcctcgg atctgcttga
     2581 gagctgtgct taggacttta ggtaggccca cagaacaagc cacacagaga tgaccaccac
     2641 agaacctgag cctcgggatg taatggattt ccagtctggg acaaaaagga acatctccca
     2701 cttcttgtaa cagggctatg aatgcggtgg tccccactgg ggtattatga gttcttgagg
     2761 tcagcatctt tggcctcctg gaaaaagaga agacaagtga actgtgtgcc ctaccggaat
     2821 gcccacatcc ccaggcctct cagtctctct gcctcaatct gccctccttt cgcctctcca
     2881 ccccgtgtcc tgttattttt agcttcagat ttttagtgca ggatcttttc cctggcccag
     2941 ctacatcctc catataccac cagccacctc cttcccttca tgcgctcagg gttcatccct
     3001 tcctcccagt ctctaccagc tatgtctgta gagacagcgg tcctctgcct ccctctctaa
     3061 ttcttctccc ttctctggtc tcttgtgtct tttcttacct cttcctgagc ctctctcatc
     3121 tttccttcat atctgagata cccgcagcct ctgactctgg cctctcctcc cgcgtgccca
     3181 gcctttgacc tccgggggct gccttcctgc catcaccttt actgtccctg caggctagag
     3241 gagccactaa gttggcggga ggtacggttc catgtcttgg tccgggtcga gttcttgctc
     3301 ctgttcctcc ttcagcctct gtcggatctc ctcagcttct gcccggtcct cgtcctcata
     3361 gtactctttg tctaggcgct ctaggtgtgc catctgcacc agggcctcct ggcggtagtc
     3421 agtctcatcg gcacaggggt tgtccagcag caccaaggcc ctcagcttgg gcaggtcccg
     3481 cagtttggcc agctccccca ggtcactgat catgttactc ctgtatatga ggtcgggagg
     3541 ggttaacgca ggaaaatggg ctctgagtgt tagctacaga tcgggcttcc tttgtaagaa
     3601 tacctcctgg aagccactct gggccctgga agcagtgatg gatgaggacc agccttggca
     3661 tgatgcaatt ccctcattcc tcctccaccc tttagagtcc ctaaatgccg tgcaatgtgt
     3721 tcttttaaaa ctttggagca gcaaaagcta ggcctcatcc tgtcttatat ttggagaaga
     3781 ggggcaaatg tcccatacag gagcagacat tttctgtgtc agccatacca ccataggggg
     3841 ttggaaggat tgggcgagca gaggaggagc tcggtacatc gatggtgaag ggggcggccg
     3901 cggagcctgc ttttttgtac aaacttgtga taattcgata tcaagcttat cgatggtcga
     3961 ggctagtctc gtgatcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg
     4021 tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg
     4081 aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt
     4141 acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata
     4201 taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc
     4261 aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt
     4321 aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc
     4381 tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt
     4441 tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc
     4501 aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc
     4561 gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg
     4621 gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac
     4681 ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt
     4741 gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt atataattca
     4801 cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg ccgcttgctg
     4861 tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc
     4921 ttttatctct atcctttgat gcacacaata gagggttgct actgtattat ataatgatct
     4981 aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc
     5041 ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca
     5101 tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca
     5161 tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta
     5221 gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt
     5281 gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt
     5341 caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg
     5401 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     5461 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     5521 atctggtcta accagagaga cccagtacag gcaaaaagca gctgcttata tgcaggatct
     5581 gagggctcgc cactccccag tcccgcccag gccacgcctc cctggaaagt ccccagcgga
     5641 aagtcccttg tagcaagctc gatatcagca gttcttgaag tactccggat gcagctctcg
     5701 ggccacgtga tgaaatgcta ggcggctgtc aaacctccac tctaacactt ctctctccgg
     5761 gtcatccatc ccatgcaggc tcacagggtg taacaagctg gtgttctctc ctttattggc
     5821 ctcttctacc ttatctggct caactggtac tagcttgtag caccatccaa aggtcagtgg
     5881 atatctgacc cctggccctg gtgtgtagtt ctgctaatca gggaagtagc cttgtgtgtg
     5941 gtagatccac agatcaagga tatcttgtct tctttgggag tgaattagcc cttccaacta
     6001 ctaagtttgt agtacatatt taacaaatac aatttcttta aaatgaaaat aattcagagg
     6061 aatcacaggt ttagagtaaa tgaaaccaca ggtaattggc agtggtaata gggtatgggg
     6121 tgggaagttt gggatgattt tggttagctt gagttatcca gttgatccag acatgataag
     6181 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     6241 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     6301 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     6361 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctctagt caaggcacta
     6421 tacatcaaat attccttatt aaccccttta caaattaaaa agctaaaggt acacaatttt
     6481 tgagcatagt tattaatagc agacactcta tgcctgtgtg gagtaagaaa aaacagtatg
     6541 ttatgattat aactgttatg cctacttata aaggttacag aatatttttc cataattttc
     6601 ttgtatagca gtgcagcttt ttcctttgtg gtgtaaatag caaagcaagc aagagttcta
     6661 ttactaaaca cagcatgact caaaaaactt agcaattctg aaggaaagtc cttggggtct
     6721 tctacctttc tcttcttttt tggaggagta gaatgttgag agtcagcagt agcctcatca
     6781 tcactagatg gcatttcttc tgagcaaaac aggttttcct cattaaaggc attccaccac
     6841 tgctcccatt catcagttcc ataggttgga atctaaaata cacaaacaat tagaatcagt
     6901 agtttaacac attatacact taaaaatttt atatttacct tagagcttta aatctctgta
     6961 ggtagtttgt ccaattatgt cacaccacag aagtaaggtt ccttcacaaa gatccggggc
     7021 ccactcataa atccagttgc cgccacggta gccaatcacc gtatcgtata aatcatcgtc
     7081 ggtacgttcg gcatcgctca tcacaatacg tgcctggacg tcgaggattt cgcgtgggtc
     7141 aatgccgcgc cagatccaca tcagacggtt aatcatgcga taccagtgag ggatggtttt
     7201 accatcaagg gccgactgca caggcggttg tgcgccgtga ttaaagcggc ggactagcgt
     7261 cgaggtttca ggatgtttaa agcggggttt gaacagggtt tcgctcaggt ttgcctgtgt
     7321 catggatgca gcctccagaa tacttactgg aaactattgt aacccgcctg aagttaaaaa
     7381 gaacaacgcc cggcagtgcc aggcgttgaa aagattagcg accggagatt ggcgggacga
     7441 atacgacgcc catatcccac ggctgttcaa tccaggtatc ttgcgggata tcaacaacat
     7501 agtcatcaac cagcggacga ccagccggtt ttgcgaagat ggtgacaaag tgcgcttttg
     7561 gatacatttc acgaatcgca accgcagtac caccggtatc caccaggtca tcaataacga
     7621 tgaagccttc gccatcgcct tctgcgcgtt tcagcacttt aagctcgcgc tggttgtcgt
     7681 gatcgtagct ggaaatacaa acggtatcga catgacgaat acccagttca cgcgccagta
     7741 acgcacccgg tacagaccgc cacggcttac ggcaataatg cctttccatt gttcagaagg
     7801 catcagtcgg cttgcgagtt tacgtgcatg gatctgcaac atgtcccagg tgacgatgta
     7861 tttttcgctc atgtgaagtg tcccagcctg tttatctacg gcttaaaaag tgttcgaggg
     7921 gaaaataggt tgcgcgagat tatagagatc tggcgcacta aaaaccagta tttcacatga
     7981 gtccgcgtct ttttacgcac tgcctctccc tgacgcggga taaagtggta ttctcaaaca
     8041 tatctcgcaa gcctgtcttg tgtccaagct ttttgcaaaa gcctaggcct ccaaaaaagc
     8101 ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
     8161 aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
     8221 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac
     8281 ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca
     8341 tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca
     8401 cattccacag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8461 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8521 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8581 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8641 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8701 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8761 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8821 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8881 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8941 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9001 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9061 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9121 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9181 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9241 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9301 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9361 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9421 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9481 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9541 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9601 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9661 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9721 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9781 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9841 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9901 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9961 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10021 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10081 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10141 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10201 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10261 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10321 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10381 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    10441 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    10501 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    10561 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    10621 atcagagcag attgtactga gagtgcacca ttcgacgctc tcccttatgc gactcctgca
    10681 ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg
    10741 catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
    10801 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
    10861 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
    10921 cgtagaggat ctggctagcg atgaccctgc tgattggttc gctgaccatt tccgggtgcg
    10981 ggacggcgtt accagaaact cagaaggttc gtccaaccaa accgactctg acggcagttt
    11041 acgagagaga tgatagggtc tgcttcagta agccagatgc tacacaatta ggcttgt
//
