LOCUS       sequence-48908-e        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MECR
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc ctgcccgggt ccgggcgctt gtctatgggc accacgggga tccagccaag
      121 gtcgtcgaac tcaagaacct ggagctagct gctgtgagag gatcagatgt ccgtgtgaag
      181 atgctggcgg cccctatcaa tccatctgac ataaatatga tccaaggaaa ctacggactc
      241 cttcctgaac tgcctgctgt tggagggaac gaaggtgttg cacaggtggt agcggtgggc
      301 agcaatgtga ccgggctgaa gccaggagac tgggtgattc cagcaaatgc tggtttagga
      361 acctggcgga ccgaggctgt gttcagcgag gaagcactga tccaagttcc gagtgacatc
      421 cctcttcaga gcgctgccac cctgggtgtc aatccctgca cagcctacag gatgttgatg
      481 gacttcgagc aactgcagcc aggggattct gtcatccaga atgcatccaa cagcggagtg
      541 gggcaagcgg tcatccagat cgccgcagcc ctgggcctaa gaaccatcaa tgtggtccga
      601 gacagacctg atatccagaa gctgagtgac agactgaaga gtctgggggc tgagcatgtc
      661 atcacagaag aggagctaag aaggcccgaa atgaaaaact tctttaagga catgccccag
      721 ccacggcttg ctctcaactg tgttggtggg aaaagctcca cagagctgct gcggcagtta
      781 gcgcgtggag gaaccatggt aacctatggg gggatggcca agcagcccgt cgtagcctct
      841 gtgagcctgc tcatttttaa ggatctcaaa cttcgaggct tttggttgtc ccagtggaag
      901 aaggatcaca gtccagacca gttcaaggag ctgatcctca cactgtgcga tctcatccgc
      961 cgaggccagc tcacagcccc tgcctgctcc caggtcccgc tgcaggacta ccagtctgcc
     1021 ttggaagcct ccatgaagcc cttcatatct tcaaagcaga ttctcaccat gtgacagtaa
     1081 aggtggatac ggatccgaa
//
