LOCUS       sequence-48911-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HSD17B8
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             775..795
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             796..813
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             814..837
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgcagaacc gactccgctc cgcactggcc ttggtcacag gtgcggggag cggcatcggc
       61 cgagcggtca gtgtacgcct ggccggagag ggggccaccg tagctgcctg cgacctggac
      121 cgggcagcgg cacaggagac ggtgcggctg ctgggcgggc cagggagcaa ggaggggccg
      181 ccccgaggga accatgctgc cttccaggct gacgtgtctg aggccagggc cgccaggtgc
      241 ctgctggaac aagtgcaggc ctgcttttct cgcccaccat ctgtcgttgt gtcctgtgcg
      301 ggcatcaccc aggatgagtt tctgctgcac atgtctgagg atgactggga caaagtcata
      361 gctgtcaacc tcaagggcac cttcctagtc actcaggctg cagcacaagc cctggtgtcc
      421 aatggttgtc gtggttccat catcaacatc agtagcatcg taggaaaggt ggggaacgtg
      481 gggcagacaa actatgcagc atccaaggct ggagtgattg ggctgaccca gaccgcagcc
      541 cgggagcttg gacgacatgg gatccgctgt aactctgtcc tcccagggtt cattgcaaca
      601 cccatgacac agaaagtgcc acagaaagtg gtggacaaga ttactgaaat gatcccgatg
      661 ggacacttgg gggaccctga ggatgtggca gatgtggtcg cattcttggc atctgaagat
      721 agtggataca tcacagggac ctcagtggaa gtcactggag gtcttttcat ggcagagaac
      781 ctctacttcc aatcgcacca tcatcaccac catgattaca aggatgacga cgataagtga
      841 ggatcc
//
