LOCUS       sequence-489136-       13573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stably expresses ALDH18A1 CDS with its endogenous 5'UTR and 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13573)
  AUTHORS   Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, 
            Ting AY, Weissman JS
  TITLE     Proximity-specific ribosome profiling reveals the logic of localized
            mitochondrial translation.
  JOURNAL   Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 
            10.1016/j.cell.2025.08.002.
  PUBMED    40876456
REFERENCE   2  (bases 1 to 13573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002."
COMMENT     Sequence Label: pJL163
FEATURES             Location/Qualifiers
     source          1..13573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2610..3198
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2716..2727
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3125..3145
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3223..3825)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3309..3329)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3806..3825
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3837..4337)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3900..3918)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        4424..5602
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4655..5593
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5550..5570
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     LTR             9378..9558
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9584..9601)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9590..9814
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9860..10288
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9947..9966)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10157..10178
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10297..10317)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10302..10631
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10482..10617
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10544..10563
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10679..10726
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10745..11119
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11249..11370
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11286..11305)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11340..11359
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11419..11435)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11419..11435)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11432..11454)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11443..11459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11467..11473,11474..11491,11492..11497))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11512..11533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11650..11667)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11821..12409)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11901..11920)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12580..13371,13372..13440))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13203..13222
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13441..13545)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagcgatg cggggaggcg gcccaaaggg agatccgact
     2641 cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga
     2701 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
     2761 ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac
     2821 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     2881 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
     2941 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
     3001 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
     3061 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
     3121 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
     3181 gtaatccaga ggttgattac cgataagctt gatatcgaat tcttaactgg caccgggctt
     3241 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
     3301 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
     3361 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
     3421 ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg
     3481 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
     3541 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
     3601 gaccgccacg gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
     3661 cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt
     3721 gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac
     3781 gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatggtgg taccccctgg
     3841 ggagagaggt cggtgattcg gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc
     3901 ttgcagaatg cggaacaccg cgcgggcagg aacagggccc acactaccgc cccacacccc
     3961 gcctcccgca ccgccccttc ccggccgctg ctctcggcgc gccctgctga gcagccgcta
     4021 ttggccacag cccatcgcgg tcggcgcgct gccattgctc cctggcgctg tccgtctgcg
     4081 agggtactag tgagacgtgc ggcttccgtt tgtcacgtcc ggcacgccgc gaaccgcaag
     4141 gaaccttccc gacttagggg cggagcagga agcgtcgccg gggggcccac aagggtagcg
     4201 gcgaagatcc gggtgacgct gcgaacggac gtgaagaatg tgcgagaccc agggtcggcg
     4261 ccgctgcgtt tcccggaacc acgcccagag cagccgcgtc cctgcgcaaa cccagggctg
     4321 ccttggaaaa ggcgcaaatc gattgcaaag atggataaag ttttaaacag agaggaatct
     4381 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     4441 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4501 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4561 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4621 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     4681 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     4741 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     4801 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     4861 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     4921 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     4981 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     5041 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     5101 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     5161 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     5221 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     5281 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     5341 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     5401 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     5461 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     5521 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     5581 tcttccattt caggtgtcgt gacctgcagg atcgtgcggg acgtggacgg aagaaaaaag
     5641 agagtgagcg agcgccggaa tcagcccggc gtggagtgcg gacccgcggt agtgccaggg
     5701 ggcgaaggcg gcggtggtga ggaagatact ttggttagtg accacatcgc agcatgttga
     5761 gtcaagttta ccgctgtggg ttccagccct tcaaccaaca tcttctgccc tgggtcaagt
     5821 gtacaaccgt cttcagatct cattgtatcc agccttcagt aatcagacat gttcgttctt
     5881 ggagcaacat cccgtttatc actgtacccc tcagtcgtac acatggcaag tccttcgccc
     5941 accgcagtga gctgaagcat gccaagagaa tcgtggtgaa gctcggcagt gccgtggtga
     6001 cccgagggga tgaatgtggc ctggccctgg ggcgcttggc atctattgtt gagcaggtat
     6061 cagtgctgca gaatcagggc agagagatga tgctggtgac cagtggagcc gtagcctttg
     6121 gcaaacaacg cttgcgccat gagatccttc tgtctcagag cgtgcggcag gccctccact
     6181 cggggcagaa ccagctgaaa gaaatggcaa ttccagtctt agaggcacga gcctgtgcag
     6241 ctgccggaca gagtgggctg atggccttgt atgaggctat gtttacccag tacagcatct
     6301 gtgctgccca gattttggtg accaatttgg atttccatga tgagcagaag cgccggaacc
     6361 tcaatggaac acttcatgaa ctccttagaa tgaacattgt ccccattgtc aacacaaatg
     6421 atgctgttgt ccccccagct gagcccaaca gtgacctgca gggggtaaat gttattagtg
     6481 ttaaagataa tgatagcctg gctgcccgac tggctgtgga aatgaaaact gatctcttga
     6541 ttgttctttc agatgtagaa ggcctttttg acagcccccc aggttcagat gatgcaaagc
     6601 ttattgatat attttatccc ggagatcagc agtctgtgac atttggaacc aagtctagag
     6661 tgggaatggg tggcatggaa gccaaggtga aagcagccct ctgggctttg caaggtggca
     6721 cttctgttgt tattgccaat ggaacccacc caaaggtgtc tgggcacgtc atcacagaca
     6781 ttgtggaggg gaagaaagtt ggtaccttct tttcagaagt aaagcctgca ggccctactg
     6841 ttgagcagca gggagaaatg gcgcgatctg gaggaaggat gttggccacc ttggaacctg
     6901 agcagagagc agaaattatc catcatctgg ctgatctgtt gacggaccag cgtgatgaga
     6961 tcctgttagc caacaaaaaa gacttggagg aggcagaggg gagacttgca gctcctctgc
     7021 tgaaacgttt aagcctctcc acatccaaat tgaacagcct ggccatcggt ctgcgacaga
     7081 tcgcagcctc ctcccaggac agcgtgggac gtgttttgcg ccgcacccga atcgccaaaa
     7141 acttggaact ggaacaagtg actgtcccaa ttggagttct gctggtgatc tttgaatctc
     7201 gtcctgactg tctaccccag gtggcagctt tggctatcgc aagtggcaat ggcttgttac
     7261 tcaaaggagg gaaggaggct gcacacagca accggattct ccacctcctg acccaggagg
     7321 ctctctcaat ccatggagtc aaggaggccg tgcaactggt gaataccaga gaagaagttg
     7381 aagatctttg ccgcctagac aaaatgatag atctgatcat tccacgtggc tcttcccagc
     7441 tggtcagaga catccagaaa gctgctaagg ggattccagt gatggggcac agcgaaggga
     7501 tctgtcacat gtatgtggat tccgaggcca gtgttgataa ggtcaccagg ctagtcagag
     7561 actctaaatg tgaatatcca gctgcctgta atgctttgga gactttgtta atccaccggg
     7621 atctgctcag gacaccatta tttgaccaga tcattgatat gctgagagtg gaacaggtaa
     7681 aaattcatgc aggccccaaa tttgcctcct atctgacctt cagcccctcc gaagtgaagt
     7741 cactccgaac tgagtatggg gacctggaat tatgcattga agtagtggac aacgttcagg
     7801 atgccattga ccacatccac aagtatggca gctcccacac ggatgtcatc gtcacagagg
     7861 acgaaaacac agcggagttc ttcctgcagc acgtagacag tgcctgtgtg ttctggaatg
     7921 ccagcactcg cttttctgat ggttaccgct ttggactggg agctgaagtg ggaatcagta
     7981 catcgagaat ccacgcccgg ggaccagtag gacttgaggg actgcttact actaagtggc
     8041 tgctgcgagg gaaggaccac gtggtctcag atttctcaga gcatggaagt ttaaaatatc
     8101 ttcatgagaa cctccctatt cctcagagaa acaccaactg attagtctgc tgaaacaagc
     8161 cgcctgaacg cgttaagtcg acaaagagcc aggaaaaccc gggaattttc caaaaggtct
     8221 tcacgttaaa cttgtcttat ctcaggagag agcccgctct tgtctcccag ttcctggtag
     8281 ggtctgcctg ttggaaagtg tacctggatg cttctgggct ccgtttggca atagcagtct
     8341 tggctgatgt gcacagtctg gctcccagct cacccttttt ttttaaagta agaaaatagt
     8401 tgctaccgat agggactttg ccaagtccaa ttatcttcta ggattgaaag gtgcattttc
     8461 cccataaaaa aggcgaggaa aacccatggc tgctttgtgt cacctcagtg acttacagtc
     8521 ccccttggca tttagttggt actagagcca gtcatcctta acaaatcttt tcacatttta
     8581 tttctttcac atgtagtcat cttcaaaaag gaaagatttg gaattttaga aaaggggcaa
     8641 ctcttctttt tagcattctc atcagaaagt cacaaaaatc gatggaatca tttccactgg
     8701 gaagattgac cttttgtatt tatttgtggg gtaaattaat aagcattcca gatgcttgca
     8761 gcttcctgca tccaggagat gctgtgttcc ccgtgatgca gctggaaccc aagctgcagc
     8821 aggagatgca agtttcagga tgttccccac tgagctggag gaatatctac agcagtgatg
     8881 cttgaaattt ttgtatgaat tattttgtcg tcctaccctt ttcctccaaa acaaaaatta
     8941 gaggattatt ttaatacttt ggattcttcc cccttttttg agattaatta actttaagac
     9001 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     9061 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     9121 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     9181 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     9241 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     9301 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     9361 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9421 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     9481 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     9541 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     9601 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     9661 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     9721 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     9781 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     9841 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9901 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9961 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    10021 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    10081 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    10141 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    10201 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    10261 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
    10321 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
    10381 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    10441 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
    10501 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    10561 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    10621 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
    10681 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
    10741 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
    10801 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
    10861 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
    10921 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
    10981 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
    11041 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
    11101 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
    11161 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
    11221 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
    11281 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    11341 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
    11401 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    11461 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    11521 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    11581 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    11641 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    11701 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    11761 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    11821 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    11881 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    11941 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    12001 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    12061 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    12121 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    12181 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    12241 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    12301 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    12361 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    12421 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    12481 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    12541 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    12601 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    12661 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    12721 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    12781 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    12841 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    12901 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    12961 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    13021 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    13081 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    13141 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    13201 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    13261 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    13321 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    13381 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    13441 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    13501 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    13561 agtgccacct gac
//
