LOCUS       sequence-48915-e        1753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1753)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ME1
FEATURES             Location/Qualifiers
     source          1..1753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc agcgcggcta cctgctgaca cggaaccctc acctcaacaa ggacttggcc
      121 tttaccctgg aagagagaca gcaattgaac attcatggat tgttgccacc ttccttcaac
      181 agtcaggaga tccaggttct tagagtagta aaaaatttcg agcatctgaa ctctgacttt
      241 gacaggtatc ttctcttaat ggatctccaa gatagaaatg aaaaactctt ttatagagtg
      301 ctgacatctg acattgagaa attcatgcct attgtttata ctcccactgt gggtctggct
      361 tgccaacaat atagtttggt gtttcggaag ccaagaggtc tctttattac tatccacgat
      421 cgagggcata ttgcttcagt tctcaatgca tggccagaag atgtcatcaa ggccattgtg
      481 gtgactgatg gagagcgtat tcttggcttg ggagaccttg gctgtaatgg aatgggcatc
      541 cctgtgggta aattggctct atatacagct tgcggaggga tgaatcctca agaatgtctg
      601 cctgtcattc tggatgtggg aaccgaaaat gaggagttac ttaaagatcc actctacatt
      661 ggactacggc agagaagagt aagaggttct gaatatgatg attttttgga cgaattcatg
      721 gaggcagttt cttccaagta tggcatgaat tgccttattc agtttgaaga ttttgccaat
      781 gtgaatgcat ttcgtctcct gaacaagtat cgaaaccagt attgcacatt caatgatgat
      841 attcaaggaa cagcatctgt tgcagttgca ggtctccttg cagctcttcg aataaccaag
      901 aacaaactgt ctgatcaaac aatactattc caaggagctg gagaggctgc cctagggatt
      961 gcacacctga ttgtgatggc cttggaaaaa gaaggtttac caaaagagaa agccatcaaa
     1021 aagatatggc tggttgattc aaaaggatta atagttaagg gacgtgcttc cttaacacaa
     1081 gagaaagaga agtttgccca tgaacatgaa gaaatgaaga acctagaagc cattgttcaa
     1141 gaaataaaac caactgccct cataggagtt gctgcaattg gtggtgcatt ctcagaacaa
     1201 attctcaaag atatggctgc cttcaatgaa cggcctatta tttttgcttt gagtaatcca
     1261 actagcaaag cagaatgttc tgcagagcag tgctacaaaa taaccaaggg acgtgcaatt
     1321 tttgccagtg gcagtccttt tgatccagtc actcttccaa atggacagac cctatatcct
     1381 ggccaaggca acaattccta tgtgttccct ggagttgctc ttggtgttgt ggcgtgtgga
     1441 ttgaggcaga tcacagataa tattttcctc actactgctg aggttatagc tcagcaagtg
     1501 tcagataaac acttggaaga gggtcggctt tatcctcctt tgaataccat tagagatgtt
     1561 tctctgaaaa ttgcagaaaa gattgtgaaa gatgcatacc aagaaaagac agccacagtt
     1621 tatcctgaac cgcaaaacaa agaagcattt gtccgctccc agatgtatag tactgattat
     1681 gaccagattc tacctgattg ttattcttgg cctgaagagg tgcagtgaca gtaaaggtgg
     1741 atacggatcc gaa
//
