LOCUS       sequence-48916-e        1045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1045)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MCAT
FEATURES             Location/Qualifiers
     source          1..1045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg gccagtgctc cgtgctgctc ttcccgggcc agggcagcca ggtggtgggc
      121 atgggccgcg gtctgctcaa ctacccgcgc gtccgcgaac tctacgccgc cgcccgccgc
      181 gtgctgggct acgacctgct ggaactgagc ctgcacgggc cgcaggagac cctggaccgc
      241 accgtgcact gtcagcccgc gatcttcgtg gcatcgctgg ccgctgtcga gaaactacat
      301 cacctgcagc cctcggtgat tgagaactgt gttgctgctg ctggattcag tgtgggagag
      361 tttgcagccc tagtgtttgc cggagccatg gaatttgctg aaggtttgta tgcagtgaaa
      421 atccgagctg aggccatgca ggaagcttca gaagctgtcc ccagtgggat gctgtctgtc
      481 ctcggccagc ctcagtccaa gttcaacttc gcctgtttgg aagcccggga acactgcaag
      541 tctttaggca tagagaaccc cgtatgtgaa gtgtccaact acctctttcc agattgcagg
      601 gtgatttcag gacaccaaga ggctctacgg tttctccaga agaattcctc taagtttcat
      661 ttcagacgca ccaggatgtt gccggttagt ggcgcattcc acacccgcct catggagcca
      721 gccgtggagc ccctgacgca agctttaaag gcagtcgaca ttaagaagcc tctggtttct
      781 gtctactcca acgtccacgg gcatagatac aggcatcccg ggcacatcca caagctgctg
      841 gcccagcagc tggtctcccc agtgaagtgg gagcagacga tgcatgccat atacgaaagg
      901 aaaaagggca gggggttccc ccaaactttc gaagtaggcc ctggcaggca gctgggagcc
      961 atcctgaaga gctgtaacat gcaggcctgg aagtcctaca gcgccgtgga tgtgctgtga
     1021 cagtaaaggt ggatacggat ccgaa
//
